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1.
PLoS Genet ; 7(6): e1002123, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21698131

ABSTRACT

The Escherichia coli chromosome is organized into four macrodomains, the function and organisation of which are poorly understood. In this review we focus on the MatP, SeqA, and SlmA proteins that have recently been identified as the first examples of factors with macrodomain-specific DNA-binding properties. In particular, we review the evidence that these factors contribute towards the control of chromosome replication and segregation by specifically targeting subregions of the genome and contributing towards their unique properties. Genome sequence analysis of multiple related bacteria, including pathogenic species, reveals that macrodomain-specific distribution of SeqA, SlmA, and MatP is conserved, suggesting common principles of chromosome organisation in these organisms. This discovery of proteins with macrodomain-specific binding properties hints that there are other proteins with similar specificity yet to be unveiled. We discuss the roles of the proteins identified to date as well as strategies that may be employed to discover new factors.


Subject(s)
Bacterial Proteins/metabolism , Chromosomes, Bacterial/genetics , Chromosomes, Bacterial/metabolism , DNA, Bacterial/genetics , DNA, Bacterial/metabolism , Gram-Negative Bacteria/genetics , Gram-Negative Bacteria/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Gene Expression Regulation, Bacterial
2.
Blood ; 115(13): 2610-8, 2010 Apr 01.
Article in English | MEDLINE | ID: mdl-20093403

ABSTRACT

Clonal analysis is important for many areas of hematopoietic stem cell research, including in vitro cell expansion, gene therapy, and cancer progression and treatment. A common approach to measure clonality of retrovirally transduced cells is to perform integration site analysis using Southern blotting or polymerase chain reaction-based methods. Although these methods are useful in principle, they generally provide a low-resolution, biased, and incomplete assessment of clonality. To overcome those limitations, we labeled retroviral vectors with random sequence tags or "barcodes." On integration, each vector introduces a unique, identifiable, and heritable mark into the host cell genome, allowing the clonal progeny of each cell to be tracked over time. By coupling the barcoding method to a sequencing-based detection system, we could identify major and minor clones in 2 distinct cell culture systems in vitro and in a long-term transplantation setting. In addition, we demonstrate how clonal analysis can be complemented with transgene expression and integration site analysis. This cellular barcoding tool permits a simple, sensitive assessment of clonality and holds great promise for future gene therapy protocols in humans, and any other applications when clonal tracking is important.


Subject(s)
Cell Lineage , Clone Cells/chemistry , DNA, Recombinant/analysis , Genetic Markers , Genetic Vectors/genetics , Hematopoietic Stem Cells/chemistry , Oligodeoxyribonucleotides/analysis , Retroviridae/genetics , Sequence Analysis, DNA/methods , Animals , Binomial Distribution , Cell Separation/methods , Flow Cytometry/methods , Genetic Therapy/methods , Genetic Vectors/analysis , Hematopoietic Stem Cell Transplantation , Hematopoietic Stem Cells/cytology , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Transgenes , Virus Integration
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