ABSTRACT
Predictive models of comorbidity, dyslipidemic disorders and essential arterial hypertension, in Russian adolescents aged 12 to 18 years (mean 15.48±1.53) were formulated with consideration for biochemical (lipid profiles) and genetic parameters (carrier state of gene polymorphic variants of apolipoprotein genes ApoA1 (-75G/A and +83C/T), ApoB (Ins/Del), ApoC3 (S1/S2), and ApoE (ε2/ε3/ε4). Significant prognostic risk factors for the mentioned comorbid pathologies were lipid metabolism parameters HDL-Ch, LDL-Ch, VLDL-Ch and carrier state of the +83T allele of the ApoA1 gene and Del allele of the ApoB gene. The obtained mathematical model is characterized by high predictive accuracy: the percentage of correct classification or the rate of correct assignment of each participant to the proper group was 96.33%.
Subject(s)
Apolipoprotein A-I/genetics , Apolipoprotein B-100/genetics , Dyslipidemias/diagnosis , Essential Hypertension/diagnosis , Genetic Predisposition to Disease , Models, Statistical , Polymorphism, Genetic , Adolescent , Alleles , Apolipoprotein A-I/immunology , Apolipoprotein B-100/immunology , Apolipoprotein C-III/genetics , Apolipoprotein C-III/immunology , Apolipoproteins E/genetics , Apolipoproteins E/immunology , Carrier State , Child , Cholesterol, HDL/blood , Cholesterol, LDL/blood , Cholesterol, VLDL/blood , Discriminant Analysis , Dyslipidemias/blood , Dyslipidemias/genetics , Dyslipidemias/immunology , Essential Hypertension/blood , Essential Hypertension/genetics , Essential Hypertension/immunology , Female , Gene Expression , Gene Frequency , Humans , Male , Prognosis , Risk Factors , Russia , Triglycerides/bloodABSTRACT
We carried out a comparative analysis of circadian rhythms of melatonin secretion in Caucasian menopausal women with and without insomnia depending on the 3111T/C polymorphism of the Clock gene. Melatonin levels was measured in the saliva 4 times a day (06.00-07.00, 12.00-13.00, 18.00-19.00, and 23.00-00.00 h). Carriers of the TT genotype with insomnia demonstrated significantly higher level of melatonin in the early morning hours compared to the carriers of the minor allele C (12.60±7.58 and 8.98±8.62 pg/ml, respectively, p=0.023). In the control group, no statistically significant differences were revealed. The carriers of the TT genotype with sleep disorders have higher morning melatonin level compared to control group women (12.60±7.58 and 5.48±4.74 pg/ml, respectively, p=0.005) and low nocturnal melatonin level (6.42±4.97 and 12.52±10.40 pg/ml, respectively, p=0.039).
Subject(s)
CLOCK Proteins/genetics , Circadian Rhythm/genetics , Melatonin/metabolism , Menopause/genetics , Polymorphism, Single Nucleotide , Sleep Initiation and Maintenance Disorders/genetics , Alleles , CLOCK Proteins/metabolism , Case-Control Studies , Female , Gene Expression Regulation , Gene Frequency , Genotype , Humans , Middle Aged , Russia , Saliva/chemistry , Saliva/metabolism , Sleep Initiation and Maintenance Disorders/metabolism , Sleep Initiation and Maintenance Disorders/physiopathologyABSTRACT
Comparative analysis of the frequency distributions of genotypes and alleles of 3111T/C Clock gene polymorphism was carried out in climacteric Caucasian women with and without insomnia. Genotype TT is more incident in women with insomnia (55.5% vs. 42.6% in the control). Allele T predominated in the control and study group and its frequency is higher in women with insomnia. The OR for the risk of insomnia realization is 1.78 (95%CI 1.16-2.75). No association between genotypes and complaints of patients with insomnia is detected.
Subject(s)
CLOCK Proteins/genetics , Polymorphism, Single Nucleotide/genetics , Sleep Initiation and Maintenance Disorders/genetics , Adult , Alleles , Circadian Rhythm/genetics , Circadian Rhythm/physiology , Female , Gene Frequency/genetics , Genetic Predisposition to Disease/genetics , Genotype , Humans , Male , Middle Aged , White People/geneticsABSTRACT
Using MDR bioinformatic analysis we studied gene-gene interactions between apolipoprotein genes in adolescents with essential arterial hypertension and dyslipidemia against the background of essential arterial hypertension. Optimal models of gene-gene interactions were formed. The six-locus model was the most significant: (ApoA1(+83T), ApoA1(-75A), ApoB(Del), ApoC3(S2), ApoE(ε2), ApoE(ε4). The maximum synergism in both adolescent groups were shown for allele variants ApoA1(-75A), ApoB(Del), and ApoE(ε4). The maximum contribution to gene-gene interactions entropy was made by allelic polymorphisms ApoA1(-75A) and ApoE(ε4) and (in the comorbid pathology group) for ApoE(ε4)+ApoB(Del).
Subject(s)
Dyslipidemias/genetics , Dyslipidemias/metabolism , Hypertension/genetics , Hypertension/metabolism , Polymorphism, Genetic/genetics , Adolescent , Alleles , Apolipoprotein A-I/genetics , Apolipoproteins B/genetics , Apolipoproteins E/genetics , Child , Female , Genotype , Humans , Male , Protein Binding/genetics , Protein Binding/physiologyABSTRACT
We studied the frequency of alleles and genotypes of CAT gene -262C>T polymorphism (rs1001179) in Russian and Buryat adolescents. The frequency of -262T allele was 28.31% in Russians and 16.84% in Buryats (p<0.01). In both ethnic groups, a correlation between the study polymorphism and concentration of diene conjugates was observed. Carriers of TT-genotype of CAT gene-262C>T polymorphism had lower level of diene conjugates than carriers of CT- and CC-genotypes.
Subject(s)
Catalase/genetics , Adolescent , Female , Genotype , Humans , Male , Oxidative Stress/genetics , Polymorphism, Genetic/genetics , White PeopleABSTRACT
We studied the incidence of genotypes of polymorphic alleles (-75)G>A and (+83)C>T of apolipoprotein A1 gene in healthy Russian adolescents, residents of East Siberia. Genotyping was carried out by PCR with subsequent restriction fragment length polymorphism analysis. The incidence of allele (-75)A was 22.5%, of allele (+83)T - 7.3%. Association of allele (-75) A with high blood cholesterol level was revealed.
