[Liver mitochondria in antigenic stress in rats]. / Mitokhondrii pecheni v realizatsii antigennogo napriazheniia organizma u krys.

The penomenon of reciprocal regulation of succinate dehidrogenase activity in rat liver mitochondria was elicited in antigenic strain induced by the administration of activated lymphocytes from animals with allotransplated heart. Two coupled but opposite changes (simultaneous activation and inhibition) in succinate depended ATP synthesis and calcium transport occur. The inhibition was correlated with the activation of synthetic processes in hepatocytes. Similar changes were provoked by epinephrine (either administered i.p. or released endogenously under immobilization stress and in myocardium infarction) as well as in patients with stomach tumor. The physiological significance of the reciprocal regulation of succinate dehydrogenase is discussed.

[Predicting heart failure by intravital study of the mitochondrial energy function in patients with heart defects]. / Prognoz serdechnoi nedostatochnosti po dannym prizhiznennogo izucheniia énergeticheskoi funktsii mitokhondrii u bol'nykh porokami serdtsa.

The mitochondrial energy system (respiration and oxidative phosphorylation) was studied in 144 patients by a lifetime method for polarographic recording of respiration of tissue sections in myocardial biopsy specimens. The application of new biochemical criteria for assessing the energy system of mitochondria increases the accuracy of diagnosis of latent heart failures in the presence of cardiac malformations.

[Liver mitochondrial participation in the body adaptation reactions in a rat heart transplant]. / Uchastie mitokhondrii pecheni v adaptatsionnykh reaktsiiakh organizma pri peresadke serdtsa u krys.

Alterations in coupling of respiration and phosphorylation as well as in energy-dependent transport of calcium in liver mitochondria of recipient were studied in dynamics of antigenic response to rat heart transplantation. As compared with the normal state the reactions studied varied in opposite directions, towards activation (hyperergic step) within 1-4 days after the heart transplantation and to decrease in their activity (hyperergic step) within 6-10 days; the latter period was characterized by development of the state similar to pathological one. Increased in amount of endogenous succinic acid as well as in succinate dehydrogenase activity were apparently among the other factors responsible for activation of energy metabolism in the hyperergic state. The reactions of liver mitochondria studied were identified as unspecific adaptation of the recipient liver tissue, which might be used for diagnosis of early steps of rejection crisis.

[Changes in the ribonuclease activity of brain cell nuclei and mitochondria at different periods following removal of the superior cervical sympathetic ganglion]. / Izmenenie aktivnosti ribonukleazy v iadrakh i mitokhondriiakh mozgovoykh kletok v razlichnye sroki posle udaleniia verkhnego sheinogo simpaticheskogo uzla.

A study of the changes in the ribonuclease activity of nuclei and mitochondria of rat brain cells following 1, 3, 7, 20 and 90 days after extirpation of the right superior cervical ganglion showed an increase of enzyme activity in nuclei from the first up to the 90-th day following removal of the ganglion. During the first three days after the operation enzyme activity is higher in nuclei obtained from the intact (left) side of the brain while later on enzyme activity is more pronounced in nuclei obtained from the right (sympathectomized) side of the brain. In mitochondria enzyme activity is depressed during the whole period studied after sympathectomy (90 days).

[Quantitative changes in the nucleic acid nuclear fraction of rat brain cells following removal of the superior cervical sympathetic ganglion]. / Kolichestvennye izmeneniia nukleinovykh kislot iadernoi fraktsii kletok golovnogo mozga krys pri udalenii verkhnego sheinogo simpaticheskogo uzla.

The nucleic acid content of the nuclear fraction of cerebral hemispheres was studied 1, 3, 7, 20 and 90 days after unilateral removal of superior cervical ganglion. The results obtained indicate that the content of nuclear RNA is significantly reduced in the desympathized side 1 day after removal of the ganglion. Seven days after sympathectomy the content of RNA increases, reaching control levels. Beginning from the 20th day it decreases and remains at a low level for 90 days. This decrease is observed on both sides of the brain. The content of nuclear DNA is significantly reduced seven days following removal of superior cervical ganglion. The possible mechanisms of the alterations observed are discussed in connection with changes in correlation of nucleic acids with nuclear proteins.