Unveiling therapeutic potential: Adipose tissue-derived mesenchymal stem cells and their exosomes in the management of diabetes mellitus, wound healing, and chronic ulcers.

Diabetes mellitus (DM) is a pervasive global health issue with substantial morbidity and mortality, often resulting in secondary complications, including diabetic wounds (DWs). These wounds, arising from hyperglycemia, diabetic neuropathy, anemia, and ischemia, afflict approximately 15% of diabetic patients, with a considerable 25% at risk of lower limb amputations. The conventional approaches for chronic and diabetic wounds management involves utilizing various therapeutic substances and techniques, encompassing growth factors, skin substitutes and wound dressings. In parallel, emerging cell therapy approaches, notably involving adipose tissue-derived mesenchymal stem cells (ADMSCs), have demonstrated significant promise in addressing diabetes mellitus and its complications. ADMSCs play a pivotal role in wound repair, and their derived exosomes have garnered attention for their therapeutic potential. This review aimed to unravel the potential mechanisms and provide an updated overview of the role of ADMSCs and their exosomes in diabetes mellitus and its associated complications, with a specific focus on wound healing.

Chitosan-based Schiff base-metal (Fe, Cu, and Zn) complexes mitigate the negative consequences of drought stress on pomegranate fruits.

Drought is one of the major environmental stresses that impairs fruit productivity and quality. The proper management of minerals can, however, assist plant to maintain their growth even under drought incidents, and is considered one of the encouraging approaches to refine the drought tolerance of plants. The beneficial effects of chitosan (CH)-based Schiff base-metal complexes (e.g., CH-Fe, CH-Cu and CH-Zn) in reducing the harmful impacts of different levels of drought stress on the growth and productivity of 'Malase Saveh' pomegranate cultivar were examined. All CH-metal complexes displayed favorable effects on the yield- and growth-related attributes of pomegranate trees cultivated under well-watered and different drought situations, with the best effects were observed with CH-Fe application. Specifically, leaves of CH-Fe-treated pomegranate plants showed higher concentrations of photosynthetic pigments [chlorophyll a (Chl a), Chl b, Chl a+b, and carotenoids by 28.0, 29.5, 28.6 and 85.7%, respectively] and microelements (Fe by 27.3%), along with increased levels of superoxide dismutase (by 35.3%) and ascorbate peroxidase (by 56.0%) enzymatic activities relative to those of CH-Fe-non-treated pomegranate plants under intense drought stress. CH-Fe-treated drought-stressed pomegranate leaves showed high increment of abscisic acid (by 25.1%) and indole-3-acetic acid (by 40.5%) relative to CH-Fe-non-treated pomegranates. The increased contents of total phenolics, ascorbic acid, total anthocyanins, and titratable acidity (by 24.3, 25.8, 9.3 and 30.9%, respectively) in the fruits of CH-Fe-treated drought-stressed pomegranates indicated the advantageousness of CH-Fe on the enhancement of fruit nutritional qualities. Collectively, our results prove the explicit functions of these complexes, particularly CH-Fe, in the control of drought-induced negative effects on pomegranate trees grown in semi-arid and dry areas.

Utilizing an Emotional Robot Capable of Lip-Syncing in Robot-Assisted Speech Therapy Sessions for Children with Language Disorders.

This study scrutinizes the impacts of utilizing a socially assistive robot, the RASA robot, during speech therapy sessions for children with language disorders. Two capabilities were developed for the robotic platform to enhance children-robot interactions during speech therapy interventions: facial expression communication (containing recognition and expression) and lip-syncing. Facial expression recognition was conducted by training several well-known CNN architectures on one of the most extensive facial expressions databases, the AffectNet database, and then modifying them using the transfer learning strategy performed on the CK+ dataset. The robot's lip-syncing capability was designed in two steps. The first step was concerned with designing precise schemes of the articulatory elements needed during the pronunciation of the Persian phonemes (i.e., consonants and vowels). The second step included developing an algorithm to pronounce words by disassembling them into their components (including consonants and vowels) and then morphing them into each other successively. To pursue the study's primary goal, two comparable groups of children with language disorders were considered, the intervention and control groups. The intervention group attended therapy sessions in which the robot acted as the therapist's assistant, while the control group only communicated with the human therapist. The study's first purpose was to compare the children's engagement while playing a mimic game with the affective robot and the therapist, conducted via video coding. The second objective was to assess the efficacy of the robot's presence in the speech therapy sessions alongside the therapist, accomplished by administering the Persian Test of Language Development, Persian TOLD. According to the first scenario, playing with the affective robot is more engaging than playing with the therapist. Furthermore, the statistical analysis of the study's results indicates that participating in robot-assisted speech therapy (RAST) sessions enhances children with language disorders' achievements in comparison with taking part in conventional speech therapy interventions.

Mesenchymal stem cells-derived exosomes as a promising new approach for the treatment of infertility caused by polycystic ovary syndrome.

Polycystic ovary syndrome (PCOS) is a multifactorial metabolic and most common endocrine disorder that its prevalence, depending on different methods of evaluating PCOS traits, varies from 4% to 21%. Chronic low-grade inflammation and irregular apoptosis of granulosa cells play a crucial role in the pathogenesis of PCOS infertility. Mesenchymal stem cells (MSCs)-derived exosomes and extracellular vesicles (EVs) are lipid bilayer complexes that act as a means of intercellular transferring of proteins, lipids, DNA and different types of RNAs. It seems that this nanoparticles have therapeutic effects on the PCOS ovary such as regulating immunity response, anti-inflammatory (local and systemic) and suppress of granulosa cells (GCs) apoptosis. Although there are few studies demonstrating the effects of exosomes on PCOS and their exact mechanisms is still unknown, in the present study we reviewed the available studies of the functions of MSC-derived exosome, EVs and secretome on apoptosis of granulosa cells and inflammation in the ovary. Therefore, the novel cell-free therapeutic approaches for PCOS were suggested in this study.

Plants adapted to arid areas: specialized metabolites.

Drought stress affects growth, morphological and biochemical properties in plants. To develop resistance and adapt to drought, plants need metabolic changes. Adaptations to stress involve changes to gene expression that activate metabolic processes that promote drought resistance and that may include biosynthesis and accumulation of specialized metabolites. Such adaptations in specialized metabolism may be important mechanisms leading to plant stress resistance and involve production of phenolics, flavonoids, terpenoid and nitrogen containing compounds that are species and genotype specific. Most plants having special metabolites to adapt to drought stress belong to different botanical families. C3, C4 and CAM plants, apply both morphological and metabolic mechanisms to adapt to drought as well as to accumulate specialized metabolites. Generally, medicinal plants increase their functional metabolites content, when exposed to drought stress. During drought stress, transcription factors and related pathways for biosynthesis of phenolics, flavonoids, anthocyanins as well as for stress jasmonate and abscisic acid stress hormones are activated.

The use of nanotechnology to increase quality and yield of fruit crops.

Nanotechnology is currently being widely employed in agriculture and horticulture. The most widely investigated and developed factor related to fruit trees is nanofertilizers (NFs), which play very important roles in increasing vegetative growth, improving reproductive growth and flowering, thereby increasing productivity, product quality and ultimately increasing shelf-life and decreasing fruit waste. These nanomaterials, which are generally sprayed at low concentrations on trees at different time intervals and in frequent sessions, are also considered as growth stimulants. Macro- and micro-scale NFs such as zinc, boron, chitosan, and fertilizer nanocomposites such as ZnFeMnB (zinc, iron, manganese, boron), NPKMg (nitrogen, phosphorus, potassium, magnesium), and calcite have been shown to significantly improve the vegetative and reproductive traits of fruit trees such as pomegranate, strawberry, mango, date, coffee and grape. Knowledge on the effects of NFs on fruit trees and biological reasons for their effects on different traits is incomplete and there is an urgent need for extensive research on these topics. © 2019 Society of Chemical Industry.

Effects of postharvest polyamine application and edible coating on maintaining quality of mango (Mangifera indica L.) cv. Langra during cold storage.

Mango is a tropical fruit which is sensitive to chilling injury. The present work investigated the potential of edible coatings of chitosan and polyamine spermidine in increasing shelf life and quality of mango. The control fruits (treated with distilled water) and the mango fruits treated with different concentrations of chitosan (0.5%, 1.0%, and 2.0%) and spermidine (0.5, 1.0, and 2.0 mM) were studied to improve postharvest characteristics and quality maintenance during cold storage. Parameters such as firmness, weight loss, fungal contamination, total phenol, antioxidant activity, vitamin C, pH, total soluble solids (TSS), titratable acidity (TA), flavor index, color index, and ethylene production were measured after at harvest (0), 8, 16, and 24 days of storage at 15 ± 2°C and relative humidity of 85%-90%. Chitosan and spermidine delayed water loss, firmness, and fungal contamination. Application of chitosan containing ascorbic acid significantly increased phenolic content and antioxidant activity compared to the control plants. It also changed soluble solid content, TA, pH of pulp, and sugar content and decreased ethylene production. The obtained results suggested that chitosan (2%) and spermidine (2 mM) had potential to improve firmness and delay deterioration processes of "Langra" mango after harvest.

Effects of postharvest treatments with chitosan and putrescine to maintain quality and extend shelf-life of two banana cultivars.

Chitosan (1.0% and 2.0%) and putrescine (1.0 and 2.0 mmol/L) treatments were used to investigate the effects of these compounds on the postharvest quality and shelf-life of two banana cultivars, "Native" and "Cavendish." Fruits were stored at 15 ± 2°C and a relative humidity of 85%-90% during a 20-day period. In the controls, increases in weight loss, microbial population, total soluble solids, and ethylene production and decreases in firmness, ascorbic acid contents, and fruit lightness occurred gradually during storage. All these changes were delayed significantly after treatments with chitosan and putrescine. Application of putrescine and chitosan also caused small increases in phenolic compound contents and antioxidant activity at the end of the storage period. Results obtained suggest that a treatment with 1% chitosan is effective in improving the postharvest quality and shelf-life of banana, and open the possibility that lower concentrations of chitosan may be also effective.