Optimization and Comparison of Microwave-Assisted Extraction, Supercritical Fluid Extraction, and Eucalyptus Oil-Assisted Extraction of Polycyclic Aromatic Hydrocarbons from Soil and Sediment.

Polycyclic aromatic hydrocarbons (PAHs) are persistent organic compounds of major concern that mainly accumulate in soils and sediments, and their extraction from environmental matrices remains a crucial step when determining the extent of contamination in soils and sediments. The objective of the present study was to compare the extraction of PAHs (phenanthrene, pyrene, chrysene, and benzo[a]pyrene) from spiked soil and sediment using supercritical fluid extraction (SFE) with ethanol as the modifier, microwave-assisted extraction (MAE), and eucalyptus oil-assisted extraction (EuAE). Recoveries of PAHs were comparable between the three methods, and >80% of applied pyrene, chrysene and benzo[a]pyrene were recovered. The most efficient method of extracting PAHs from naturally incurred soils with different levels of contamination was SFE. A longer extraction time was required for the EuAE method compared with SFE and MAE under optimized conditions. However, EuAE required lower extraction temperatures (15-20 °C) compared with SFE (80 °C) and MAE (110-120 °C), and consumed less solvent than SFE and MAE. Compared with hexane/acetone used in MAE, the use of ethanol in SFE and eucalyptus oil in EuAE can be considered as more sustainable approaches to efficiently extract PAHs from spiked/naturally contaminated soils and sediments. And, although less efficient for matrices containing higher carbon content, EuAE offered a cheap, low-tech approach to extracting PAHs. Environ Toxicol Chem 2023;42:982-994. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Eucalyptus saponin- and sophorolipid-mediated desorption of polycyclic aromatic hydrocarbons from contaminated soil and sediment.

The potential for biosurfactant-mediated desorption of polyaromatic hydrocarbons (PAHs) was evaluated using PAH-spiked soil and sediment. PAH desorption behaviors and toxicity of novel saponin biosurfactant extracted from Eucalyptus camaldulensis leaves and sophoro-lipid biosurfactant were investigated. Their PAH desorption efficiencies were compared with rhamnolipid biosurfactant and the industrial-chemical surfactant, Tween 20. Based on the emulsification indices, the salt tolerance of surfactants up to 30 g/L NaCl followed the order of saponin > Tween 20 > sophorolipid > rhamnolipid, while the thermal stability over the range of 15 to 50 °C was in the order of sophorolipid > rhamnolipid > saponin > Tween 20. The saponin biosurfactant emulsion demonstrated the highest stability under a wide range of acidic to basic pHs. PAH extraction percentages of saponin and sophorolipid under the optimized surfactant concentration, volume, and incubation time were 30-50% and 30-70%, respectively. PAH desorption capacities of saponin and sophorolipid were comparable to that of rhamnolipid and Tween 20 for all matrices. Sophorolipid more efficiently desorbed low molecular weight PAHs in soil and sediment compared to the other three surfactants. Microbial respiration was used to determine biosurfactant toxicity to the soil/sediment microbiome and indicated no inhibition of respiration during 60 days of incubation, suggesting that sophorolipid- and saponin-mediated remediation may be sustainable approaches to remove PAHs from contaminated soils and sediments.

Polycyclic aromatic hydrocarbon contamination in soils and sediments: Sustainable approaches for extraction and remediation.

Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic environmental pollutants that are extremely hydrophobic in nature and resistant to biological degradation. Extraction of PAHs from environmental matrices is the first and most crucial step in PAH quantification. Extraction followed by quantification is essential to understand the extent of contamination prior to the application of remediation approaches. Due to their non-polar structures, PAHs can be adsorbed tightly to the organic matter in soils and sediments, making them more difficult to be extracted. Extraction of PAHs can be achieved by a variety of methods. Techniques such as supercritical and subcritical fluid extraction, microwave-assisted solvent extraction, plant oil-assisted extraction and some microextraction techniques provide faster PAH extraction using less organic solvents, while providing a more environmentally friendly and safer process with minimum matrix interferences. More recently, more environmentally friendly methods for soil and sediment remediation have been explored. This often involves using natural chemicals, such as biosurfactants, to solubilize PAHs in contaminated soils and sediments to allow subsequent microbial degradation. Vermiremediation and microbial enzyme-mediated remediation are emerging approaches, which require further development. The following summarises the existing literature on traditional PAH extraction and bioremediation methods and contrasts them to newer, more environmentally friendly ways.