[The free radical oxidation and antioxidant defense systems as indicators of the activity of keratinocytic proliferation in psoriasis].

The free radical oxidation and antioxidant defense (AOD) systems in the keratinocyte cultures were studied in patients with psoriasis before and after the use of combinations of liposomal preparations. Hyperproliferation of keratinocytes was found to be attended by suppressed lipid peroxidation and high cell antioxidant activity. On the contrary, the liposomal combinations-induced suppression of cell proliferation was characterized by a drastic increase in prooxidant activity in reference to the AOD of epidermal keratinocyte cultures.

[The influence of partial androgen deficiency of aging men (PADAM) on the impulse regime of incretion of several hormones and mitotic activity].

This research work is devoted to an important subject--study of the impulse regime of the incretion of a series of hormones among men with partial androgen deficience of aging men (PADAM). The results of this study suggest that PADAM leads to a breakdown of the impulse regime of incretion of a series of hormones, including luteinizing hormone (LH), follicle stimulating hormone (FSH), and somatotropic hormones (STH), as well as cortisol and insulin among men of older age groups. These changes accompany the development of metabolic syndrome (X-syndrome); their development can be inversed through androgen-replacement therapy.

[Effect of testosterone concentration on the intensity of 5alpha-dihydrotestosterone and 17beta-oestradiol formation].

The culture of human male foreskin fibroblasts was incubated with various concentrations of testosterone in the medium. It has been shown that the minimum formation of 17beta-oestradiol and 5alpha-dihydrotestosterone occurs at physiological testosterone concentrations. Any deviation of testosterone concentration, both up and down, was accompanied by an increase in the formation of 17beta-oestradiol and 5alpha-dihydrotestosterone.

[Epithelial and stromal cell relation in peritumorous zone of the prostate gland cancer]. / Nekotorye osobennosti épitelia'no-stromal'nykh vzaimootnoshenii v peritumoroznoi zone raka predstatel'noi zhelezy.

This article deals with an urgent topic--assessment of peculiar characteistics of epithelial-stromal interactions in prostate gland tissues outside the tumor growth zone. On demonstrating pre-cancerous changes, this zone can either further accompany or precede the development of malignant neoplasm. The authors have established suppression of general morpho-functional activity of histogematogenous barrier components, increase in degenerative changes in epithelium, both in the vicinity of the tumor, and at a distance from it, which is, most probably, caused by dishormonal changes. We demonstrated the growth of lymphocytic-monocytic infiltration, and a decreased number of fibroblasts and endothelial cells of prostate gland tissues outside the tumor growth zone. The discovered changes of the histogematogenous barrier condition in the peritumorous zone and the benign hyperplasia can serve an additional criterion for early diagnostics of pre-cancerous changes of the prostate gland.

[The glutathione-dependent antioxidant system in rats during acute intoxication by hydrazine]. / Glutation-zavisimaia antioksidantnaia sistema u krys v usloviiakh ostrogo otravleniia proizvodnymi gidrazina.

Intensity of lipid peroxidation in the liver, kidney and brain tissues, and functioning of the glutathione system were studied in purebred rats after acute intoxication by 1.1-dimethyl hydrazine at the dose of 104.5 mg/kg of body mass (LD50) and phenyl hydrazine at the dose of 170 mg/kg of body mass (LD50). According to results of the investigation, the character and direction of changes in LPO and the antioxidant system due to intoxication by hydrazines are materially dependent on their structure and affected organs and tissues. For instance, intoxication by phenyl hydrazine markedly activates free-radical activation in all analyzed tissue with accumulation of malone dialdehyde against depletion of reduced glutathione in tissue and deactivation of enzymes involved in the antioxidant defense. Intoxication by dimethyl hydrazine was associated with moderate activation of free-radical oxidation in liver with a downward LPO trend in kidney and brain at the cost of exaggerated activity of enzymes in the glutathione system and maintenance of sufficient reserve of reduced glutathione. Hence, aryl hydrazine derivatives possess more distinct pro-oxidant properties.

[Role of heat-shock proteins in cardioprotection of patients operated on for ischemic heart disease]. / Rol' belka teplovogo shoka v kardioprotektsii u bol'nykh, operirovannykh po povodu ishemicheskoi bolezni serdtsa.

Major stress protein HSP72 is known to participate in protecting cells and organisms against harmful factors including ischemia, trauma etc. Under study was the level of HSP72 in the myocardium of 32 patients with coronary disease operated in Military-medical academy. HSP72 was detected in probes of the right atria before and after pre-cardiopulmonary bypass in all cases induction of HSP72 was observed in 40% of patients, and directly correlated with the time of cardiopulmonary bypass and standing of the disease. The cardioprotective effect of the elevated pre-operational level of HSP72 was shown to be proportionate to the lower activity of cardiospecific enzymes, creatine phosphokinase (CK-MB). It is suggested that HSP72 is involved in the mechanism of cardioprotection during cardiopulmonary bypass.

[Method for measuring glutathione system parameters in human lymphocytes]. / Metodika opredeleniia pokazatelei sistemy glutationa v limfotsitakh cheloveka.

The levels of reduced glutathion, activity of glutathion peroxidase, and glutathion S-transferase were measured in human peripheral blood mononuclears. The adapted methods permit measurements of glutathion system parameters in immunocompetent cells and can be used in patients with different immunopathological processes.

[Glutathione S-transferase P1-1 in normal and cancerous lung tissue: properties, function, and possible mechanisms for regulating activity]. / Glutation S-transferaza P1-1 v normal'noi i opukholevoi tkaniakh legkogo: svoistva, funktsii i vozmozhnye mekhanizmy reguliatsii aktivnosti.

Different forms of one of the enzymes catalyzing the xenobiotic metabolism, glutathione S-transferase P1-1 (GST P1-1), purified from normal and tumoral lung tissues, are described. Statistically significant (p < 0.05) increasing of enzyme activity in tumour, as compared with normal tissue, is shown. Molecular weights and isoelectric points of the enzyme two forms were characterized. Statistically significant increasing in the concentration of fatty acids with C = 18 bound to GST P1-1 in tumour tissues, was demonstrated. The possibility of regulation of GST P1-1 activity, as well as the level of its phosphorylation on serine and threonine, under the influence of the epidermal growth factor, is shown.