ABSTRACT
This paper reports the experimental data on the conservability of zolpidem and zalepion in the samples of the cadaveric liver tissue stored under different conditions. The authors describe the method that includes isolation of the substances of interest from the hepatic tissue by means of acetone extraction, their solvent and chromatographic purification, and subsequent identification with the help of the spectrophotometric technique. It is shown that zolpidem and zaleplon remain in the cadaveric hepatic tissue within 6 months after intoxication regardless of temperature which makes possible their detection, isolation, and identification by the proposed method.
Subject(s)
Acetamides/analysis , Forensic Toxicology/methods , Liver/chemistry , Pyridines/analysis , Pyrimidines/analysis , Acetamides/metabolism , Cadaver , Humans , Hypnotics and Sedatives/analysis , Hypnotics and Sedatives/metabolism , Pyridines/metabolism , Pyrimidines/metabolism , Spectrophotometry , ZolpidemABSTRACT
The authors present the results of experimental isolation of olanzapine from the biological objects in conjunction with the detailed description of the method for olanzapine extraction from cadaveric blood with the use of one of the amphiphilic solvents (e.g. acetone), purification by solvent extraction, and TLC-screening. Olanzapine was quantitatively determined by UV-spectrophotometry following its chromatography and elution. It was shown that one-step extraction from cadaveric blood by the proposed method made it possible to detect 55% of the total olanzapine content. The method was validated in the studies of blood and liver from the laboratory animals (rats) and can be recommended for the investigation in a chemical toxicological laboratory.
Subject(s)
Benzodiazepines , Forensic Toxicology/methods , Liver , Acetone , Animals , Antipsychotic Agents/analysis , Antipsychotic Agents/isolation & purification , Autopsy/methods , Benzodiazepines/analysis , Benzodiazepines/blood , Benzodiazepines/isolation & purification , Humans , Liver/chemistry , Liver/pathology , Olanzapine , Rats , Reproducibility of Results , Solvents , Spectrophotometry, Ultraviolet/methodsSubject(s)
Benzodiazepines , Dibenzothiazepines , Drug-Related Side Effects and Adverse Reactions , Antipsychotic Agents/analysis , Antipsychotic Agents/pharmacology , Antipsychotic Agents/toxicity , Benzodiazepines/analysis , Benzodiazepines/pharmacology , Benzodiazepines/toxicity , Biotransformation , Dibenzothiazepines/analysis , Dibenzothiazepines/pharmacology , Dibenzothiazepines/toxicity , Drug-Related Side Effects and Adverse Reactions/diagnosis , Drug-Related Side Effects and Adverse Reactions/etiology , Expert Testimony/methods , Forensic Toxicology/methods , Humans , Olanzapine , Quetiapine FumarateABSTRACT
A method for chemical toxic analysis of barbiturates has been developed, making use of acetone as an extracting agent and high-pressure liquid chromatography for the identification of the isolated substances. Analysis with the use of this method is preceded by extraction and chromatographic purification. The co-extractive substances do not interfere with the identification of barbiturates.
Subject(s)
Barbiturates/analysis , Chromatography, High Pressure Liquid/methods , Liver/chemistry , Barbiturates/isolation & purification , Cadaver , Chromatography, High Pressure Liquid/instrumentation , HumansABSTRACT
Conditions for chromatographic separation of melipramine with aminazine and melipramine with amitriptyline mixtures were stated. Method of chemical toxicological analysis in combined poisonings with psychotropic agents of tricyclic structure (melipramine in combinations with amitriptyline and aminazine) was suggested. Method was tested experimentally using animals.
Subject(s)
Antidepressive Agents, Tricyclic/poisoning , Animals , Antidepressive Agents, Tricyclic/analysis , Chlorpromazine/analysis , Chlorpromazine/poisoning , Chromatography, Thin Layer/methods , Imipramine/analysis , Imipramine/poisoning , Poisoning/diagnosis , Rats , Spectrophotometry, UltravioletABSTRACT
Comparative assessment of extractive ability of some solvents with different physical and chemical properties is presented. Use of amphiphilic solvent acetone to isolate aminazine gave higher results. Experimental works (on dogs) showed that use of aqueous acid solutions for aminazine extraction in chemical toxicological investigation is unsuitable.
Subject(s)
Chlorpromazine/isolation & purification , Liver/chemistry , Solvents/pharmacology , Chemical Phenomena , Chemistry, Physical , Evaluation Studies as Topic , Forensic Medicine/methods , HumansSubject(s)
Cadaver , Chlorpromazine/poisoning , Adult , Chlorpromazine/analysis , Chromatography, Thin Layer , Humans , Male , Spectrophotometry, UltravioletSubject(s)
Ampholyte Mixtures , Buffers , Liver/analysis , Nitrogen/isolation & purification , Amines/isolation & purification , Aniline Compounds/isolation & purification , Cadaver , Heterocyclic Compounds/isolation & purification , Humans , Indicators and Reagents , Methods , Neutral Red/isolation & purification , SolutionsABSTRACT
The paper is concerned with an algorithm of a program for pulmonoscintigram processing. The program developed in the algorithmic languages Basic and Assembler on the basis of this algorithm, made it possible to facilitate the data processing, to lower a physician's competence as a computer operator, to raise the objectivity of diagnosis of pulmonary diseases, etc. The program is intended for computers Vip-450, Vip-550 and MCS-560, Technicare, USA.