1.
J Gen Appl Microbiol
; 67(6): 265-268, 2021 Dec 31.
Article
in English
| MEDLINE
| ID: mdl-34373371
ABSTRACT
We describe a novel expression cassette that enables efficient and constitutive expression of the ZZ domain derived from Staphylococcus aureus protein A on the yeast cell surface to easily prepare yeast-based immunosorbents. Using this expression cassette containing the PGK1 promoter, a secretion signal derived from α-factor, and a Flo1-derived anchor protein, we successfully created a yeast-based immunosorbent for human serum albumin.