1.
Virus Res
; 22(2): 151-8, 1992 Feb.
Article
in English
| MEDLINE
| ID: mdl-1566599
ABSTRACT
Venezuelan equine encephalomyelitis virus labelled with [14C]aminoacids or [3H]uridine was purified and UV-irradiated. The irradiation led to the formation of uracil photodimers and the covalent linking of the nucleocapsid protein C to virion RNA. The inactivation of infectivity correlated with the formation of uracil dimers, whereas the RNA-protein links were formed at much higher doses of UV irradiation. The analysis of covalent RNA-protein complexes suggests that a fairly large fraction (at least one third) of the whole content of C protein is able to participate in the formation of UV-induced links, suggesting extensive contacts of RNA with protein with the nucleocapsid.
Subject(s)
Encephalitis Virus, Venezuelan Equine/radiation effects , Virus Activation/radiation effects , Animals , Centrifugation, Density Gradient , Chick Embryo , Encephalitis Virus, Venezuelan Equine/genetics , Encephalitis Virus, Venezuelan Equine/isolation & purification , Fibroblasts , Pyrimidine Dimers/chemistry , Pyrimidine Dimers/radiation effects , RNA, Viral/chemistry , RNA, Viral/radiation effects , Ultraviolet Rays , Viral Proteins/chemistry , Viral Proteins/radiation effects
2.
Mol Gen Mikrobiol Virusol
; (5): 34-6, 1988 May.
Article
in Russian
| MEDLINE
| ID: mdl-3412360