Amplitude- and frequency-dependent activation of layer II/III neurons by intracortical microstimulation.

Intracortical microstimulation (ICMS) has been used for the development of brain machine interfaces. However, further understanding about the spatiotemporal responses of neurons to different electrical stimulation parameters is necessary to inform the design of optimal therapies. In this study, we employed in vivo electrophysiological recording, two-photon calcium imaging, and electric field simulation to evaluate the acute effect of ICMS on layer II/III neurons. Our results show that stimulation frequency non-linearly modulates neuronal responses, whereas the magnitude of responses is linearly correlated to the electric field strength and stimulation amplitude before reaching a steady state. Temporal dynamics of neurons' responses depends more on stimulation frequency and their distance to the stimulation electrode. In addition, amplitude-dependent post-stimulation suppression was observed within ∼500 µm of the stimulation electrode, as evidenced by both calcium imaging and local field potentials. These findings provide insights for selecting stimulation parameters to achieve desirable spatiotemporal specificity of ICMS.

Restoration of benthic macrofauna promotes biogeochemical remediation of hostile sediments; An in situ transplantation experiment in a eutrophic estuarine-hypersaline lagoon system.

Estuarine ecosystems have very high ecological and economic value, and also act as a buffer for coastal oceans by processing nutrient inputs from terrestrial sources. However, ongoing pressures from increased urbanisation and agriculture, overlaid by climate change, has reduced inflows and increased nutrient loads that challenge the health and buffering capacity of these ecosystems. This study aimed to investigate whether restoring the bioturbating activity of Simplisetia aequisetis (Polychaeta: Nereididae) and other macrofauna could improve biogeochemical conditions in 'hostile' (i.e. hypersaline, sulfide-rich) sediments. To achieve this aim, we conducted an in situ experiment in the Coorong estuarine-lagoon ecosystem, translocating hostile hypersaline sediments, devoid of bioturbating macrofauna, to a 'healthy' (lower salinity) location where macrobenthic fauna naturally occur, and manipulating the S. aequisetis density in the sediments. Porewater, solid-phase, and diffusive equilibrium and diffusive gradient in thin-films (DET/DGT) measurements showed that bioturbation by macrobenthic fauna significantly influenced sediment biogeochemistry and remediated hostile conditions in sediment within a short time (four weeks) irrespective of S. aequisetis density. Bioturbation promoted sediment oxygenation, while salinity and the concentrations of total organic carbon and porewater sulfide, ammonium, and phosphate all decreased over time at all sediment depths. This research highlights the importance of macrobenthic communities and their functional traits for improving sediment conditions, promoting resilience to eutrophication, providing a nature-based remediation option, and in general ensuring healthy functioning of estuarine ecosystems.

Treatment of 17α­ethinylestradiol, 4­nonylphenol, and carbamazepine in wastewater using an aerobic granular sludge sequencing batch reactor.

This study quantifies the removal of a mixture of commonly detected emerging contaminants, 17α­ethinylestradiol (EE2), 4­nonylphenol (NP), and carbamazepine (CBZ) from synthetic wastewater using aerobic granular sludge in a sequential batch reactor. The emerging contaminant concentration in the influent wastewater was maintained near 500 µg/L for the duration of the experiment. Removal of EE2, NP, and CBZ is comprised of both adsorption and degradation. In general, the main removal mechanism for the emerging contaminants was initially adsorption, however it transitions to degradation as the adsorption capacity is saturated. The stabilized degradation for EE2 and NP in the SBR, was 16.09 µg/g and 20.05 µg/g, respectively. There was limited degradation of CBZ during the anaerobic phase. Both the Langmuir and Freundlich adsorption isotherms fit the data well and constants for the equations are calculated. The constants for the Langmuir Isotherm are found to be (X/m)max = 36.23, b = 0.0019 for EE2 and (X/m)max = 44.25, b = 0.0004 for CBZ. None of the tested compounds were found to be detrimental to the performance of the aerobic granules, and high macronutrient removal efficiency was maintained. The granule sludge bed and particle size were not negatively affected by EE2 and NP, however, carbamazepine appeared to interfere with the mechanism of granule formation resulting in a decrease in particle size during the experiment.