[A comparative study of the physico-chemical properties of lysine oxidase from Trichoderma sp. and Trichoderma viride Y 244-2]. / Sravnietl'noe issledovanie fiziko-khimicheskikh svoistv lizinoksidazy iz Trichoderma sp. i Trichoderma viride Y 244-2.

Some physicochemical properties of L-lysine oxidase from two strains of Trichoderma were studied. Content of metals and cofactors (Se, Zn, Cu, Fe, Co, Mn, Mo), amino acid analysis, secondary structure were estimated. The enzyme molecule from Trichoderma sp was found to contain both FAD and PQQ cofactors.

[Anti-invasive and anti-metastatic effect of lysine oxidase from Trichoderma sp. in vitro and in vivo]. / Issledovanie antiinvazivnogo i antimetastaticheskogo deistviia lizinoksiday iz Trichoderma sp. in vitro i in vivo.

A new fungal strain, Trichoderma sp., discovered in Moscow, produces the antitumor enzyme, lysine-oxidase, which demonstrates an anti-invasive effect in vitro and anti-metastatic activity in vivo. Maximal inhibition of the in vitro invasion of MM1 clone cells was obtained when the tumor cells were pretreated with 2.5 mU/ml of lysine-oxidase; the pretreatment caused a 1.9-times reduction in cell growth and a 1.6-times reduction in the invasive capacity. We studied its anti-metastatic effect on the spreading Lewis lung carcinoma (3LL) in mice from which the primary tumor had been removed. The administration of the enzyme (50 U/kg, i.v.) significantly decreased not only the extent but the number of lung metastases, as compared with the untreated mice. In addition to that, the lysine-oxidase treatment considerably increases the life-span of mice from which the primary tumor had been removed (200 days after 3LL implantation, lysine-oxidase treatment caused surviving of 50% mice in experimental group).

[A new method of purification of L-lysine-alpha-oxidase from the fungus Trichoderma]. / Novyi metod ochistki L-lizin-alpha-oksidazy griba Trichoderma.

A new procedure is developed for purification of the antitumoral enzyme L-lysyl-alpha-oxidase from Trichoderma sp. The procedure included two steps: hydrophobic chromatography on butyl sylochrome C-80 and chromatography using biospecific sorbent AH-Sepharose. The simplified procedure enabled to increase distinctly the specific enzymatic activity from 30 to 50 IU/mg in the preparation obtained with a good yield.

[Antimetastatic effect of L-lysine-alpha oxidase]. / Antimetastaticheskii éffekt L-lizin-alfa-oksidazy.

The influence of a new antitumor enzyme L-lysine alpha-oxidase on Lewis lung carcinoma spreading was studied in mice in which primary tumor had been removed. The enzyme was found to significantly decrease the extent and number of lung metastases as compared to mice which hadn't received L-lysine alpha-oxidase. This was matched by recovery of alveolar macrophages functional activity, as assessed by adenosine deaminase and 5' nucleotidase levels in these cells. Moreover, antimetastatic and cytostatic effect was confirmed by the measuring of polyamine concentration in mice erythrocytes.

[Evaluation of antileukemic activity of L-lysine-alpha oxidase from Trichoderma sp. in chemotherapeutic experiments]. / Otsenka protivoleikoznoi aktivnosti L-lizin-alfa-oksidaza iz Trichoderma sp. v khimioterapevticheskikh opytakh.

Evidences on the antileukemic effect of L-lysine alpha-oxidase from Trichoderma sp. are presented. It is inferred that enzyme needs detail studying as a potential chemotherapeutic drug both in experimental and clinical research.

[Improved method of purification of L-lysine-alpha-oxidase from the fungus Trichoderma sp]. / Usovershenstvovannaia skhema ochistki L-lizin-alpha-oksidazy griba Trichoderma sp.

Relatively rapid procedure was developed for isolation and purification of the antitumor enzyme L-lysine alpha-oxidase. The procedure involved three steps instead of six or four steps described previously, with a yield of 64.4%. The homogenous preparation was obtained as shown by gel electrophoresis and ultracentrifugation. The antitumor activity of the enzyme both in vivo and in vitro is under investigation.

[Various physico-chemical properties of L-lysine-alpha-oxidase from Trichoderma sp]. / Nekotorye fiziko-khimicheskie svoistva L-lizin-alpha-oksidazy iz Trichoderma sp.

L-lysine-alpha-oxidase, isolated from a strain of Trichoderma sp. exhibited enzymological properties suitable for a chemotherapeutic drug used in experimental oncology.

[Cytostatic effect of L-lysine-alpha-oxidase from Trichoderma harzianum Rifai and Trichoderma viride]. / Tsitostaticheskii éffekt L-lizin-alpha-oksidazy iz Trichoderma harzianum Rifai i Trichoderma viride.

L-lysine-alpha-oxidase, a new fungal enzyme catalyzing oxidative L-lysine deamination, was shown to have an inhibitory effect on the in vitro synthesis of DNA, RNA and proteins in human carcinoma ovarian (CaOv) cells.

[Comparative study of the effect of L-lysine-L-oxidase from Trichoderma harzianum Rifai and Trichoderma viride on nucleic acid synthesis in human tumor cells in vitro]. / Sravnitel'noe izuchenie vliianiia L-lizin-L-oksidazy iz Trichoderma harzianum Rifai i Trichoderma viride na sintez nukleinovykh kislot v opukholevykh kletkakh cheloveka in vitro.

L-lysine-alpha-oxidase, a new fungal enzyme catalyzing oxidative deamination of L-lysine, exerts an inhibitory effect on DNA, RNA and protein synthesis in human cells of carcinoma ovarius (CaOv) in vitro.

[Isolation and purification of L-lysine-alpha-oxidase from Trichoderma sp]. / Vydelenie i ochistka L-lizin-alpha-oksidazy iz Trichoderma sp.

An improved and relatively rapid procedure is developed for isolation and purification of a new antitumor enzyme L-lysyl-alpha-oxidase from Trichoderma sp. The method involves four steps, instead of six steps described previously, with a yield of 22.4%. The purified enzyme preparation was homogeneous as shown by polyacrylamide gel disc electrophoresis and ultracentrifugation. Physico-chemical and antitumor properties of the enzyme are under study.

[Effect of L-lysine-alpha-oxidase on the cell cycle kinetics of cultured Burkitt's lymphoma cells]. / Vliianie L-lizin-al'fa-oksidazy na kinetiku kletochnogo tsikla kul'tiviruemykh kletok limfomy Berkitta.

L-lysine-alpha-oxidase from Trichoderma sp. fungi inhibits the synthesis of DNA, RNA and protein in cultivated cells of the Burkitt lymphoma (strain P3HRj) in vitro, which results in the blocking of the cells' transfer from phase S to phase G2/M of the mitotic cycle.

[L-lysine-alpha-oxidase activity of some Trichoderma species]. / L-lizin-al'fa-oksidaznaia aktivnost' nekotorykh vidov Trichoderma.

Trichoderma cultures were tested for their ability to produce L-lysine-alpha-oxidase. The highest enzyme activity was manifested by T. harzianum (MGU), T. longibrachiatum Rifai VKM F-2025 and T. aureoviride Rifai VKM F-2026. The biosynthesis of the enzyme did not depend on the growth of the cultures and did not vary among the species.