1.
Gene Ther
; 2(2): 164-7, 1995 Mar.
Article
in English
| MEDLINE
| ID: mdl-7719933
ABSTRACT
A patient with glioblastoma multiforme (GBM) who had failed conventional therapy was treated with IL-2 gene therapy. The patient received 10 subcutaneous immunizations with autologous tumor cells and fibroblasts genetically modified to secrete IL-2 by retroviral gene transfer. An antitumor immune response mediated in part by CD8+ cytotoxic T cells was demonstrated with the patient's peripheral blood mononuclear cells. A magnetic resonance imaging (MRI) scan performed 4 weeks after the highest treatment dose revealed marked tumor necrosis. These results support the evaluation of this form of IL-2 gene therapy in additional patients with glioblastoma.
Subject(s)
Brain Neoplasms/therapy , Fibroblasts/metabolism , Fibroblasts/transplantation , Genetic Therapy , Glioblastoma/therapy , Interleukin-2/therapeutic use , Neoplastic Stem Cells/metabolism , Neoplastic Stem Cells/transplantation , Recombinant Fusion Proteins/therapeutic use , Salvage Therapy , Temporal Lobe , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Brain Neoplasms/immunology , Brain Neoplasms/pathology , Cells, Cultured/transplantation , Combined Modality Therapy , Fatal Outcome , Female , Genetic Vectors , Glioblastoma/immunology , Glioblastoma/pathology , Humans , Injections, Subcutaneous , Interleukin-2/genetics , Interleukin-2/metabolism , Isotretinoin/therapeutic use , Lomustine/administration & dosage , Magnetic Resonance Imaging , Middle Aged , Necrosis , Neoplasm Recurrence, Local , Procarbazine/administration & dosage , Radioimmunotherapy , Radiosurgery , Recombinant Fusion Proteins/metabolism , Retroviridae/genetics , T-Lymphocytes, Cytotoxic/immunology , Tamoxifen/therapeutic use , Transfection , Tumor Cells, Cultured/transplantation , Vincristine/administration & dosage
2.
Cancer Pract
; 2(3): 180-4, 1994.
Article
in English
| MEDLINE
| ID: mdl-8055021