ABSTRACT
Objective: Gallic acid (GA) is an organic acid that possesses anti-inflammatory effects as it inhibits the production of metalloproteinases, tissue plasminogen activator, growth factors and adhesion molecules. Since formation of abdominal surgery-induced adhesion bands is accompanied by inflammation, angiogenesis and cell proliferation, in the current study, we assessed potential beneficial properties of GA against adhesion bands formation in rats. Materials and Methods: Thirty-six adult male rats were assigned into six groups of six animals. After induction of anesthesia, peritoneal injury was induced using a standard method and animals received either GA (10, 25, 50 and 100 mg/kg), or normal saline, while a group of rats remained intact. Seven days after the surgery, animals were decapitated and samples were collected for pathology evaluations. Also, lipid peroxidation (TBARS) and tumor necrosis factor alpha (TNF-α) levels were determined in serum samples. Results: Our results showed that GA significantly reduced lipid peroxidation in serum samples but had no effect on TNF-α levels. Furthermore, microscopic and macroscopic injuries reduced significantly in GA-treated animals. Conclusion: Since GA reduced adhesion bands formation at microscopic and macroscopic levels, it could be considered a treatment against adhesion bands formation.
ABSTRACT
BACKGROUND: The purpose of this study was seroepidemiological and parasitological assessment of Toxocara canis infection in children and dogs in Zabol and Chabahar, Iran. METHODS: This study was a descriptive-analytic study with a simple random sampling of children under 14 yr old, referring to urban, rural, and tribal laboratories of Zabol and Chabahar, Sistan and Baluchestan Province, Iran in 2016. Demographic data, clinical, and laboratory conditions of patients were collected through interviews, questionnaires, and blood count measuring. The prevalence of IgG antibodies against T. canis was assessed by ELISA. T. canis eggs in dogs (as the original host) were also assessed by examining animal feces. Then the data were analyzed using SPSS 19 software and descriptive statistics, chi-square and ANOVA statistical tests. RESULTS: Totally, 364 patients were enrolled, of which 51.6% were female and mean±SD age of participants was 7.2 (±3.7) yr. IgG antibodies against T. canis was observed in 3.8% of cases. A significant association was found between the seroprevalence of T. canis and eosinophil (P=0.003) and red blood cell count (P=0.04). We also found a significant association between serological prevalence of T. canis and demographic parameters, such as city of residence (P=0.003), gender (P=0.04), consumption of vegetables (P=0.01), and the living place (P=0.04). Mean antibody titration was 2.2 ±1.1, with statistically significant difference among age groups (P=0.001). In addition, T. canis infection was positive in 27.5% of dogs living in the study areas. CONCLUSION: High risk of infection represented in patients referring to laboratories of Zabol and Chabahar. In addition, given the fact that dogs are the final hosts to transfer Toxocara infection to humans, this study emphasizes the need to control the population of stray dogs in the region to prevent the development of disease in the human society.
