Active soil microbial composition and proliferation are directly affected by the presence of biocides from building materials.

Combinations of biocides are commonly added to building materials to prevent microbial growth and thereby cause degradation of the façades. These biocides reach the environment by leaching from façades posing an environmental risk. Although ecotoxicity to the aquatic habitat is well established, there is hardly any data on the ecotoxicological effects of biocides on the soil habitat. This study aimed to characterize the effect of the biocides terbutryn, isoproturon, octhilinone, and combinations thereof on the total and metabolically active soil microbial community composition and functions. Total soil microbial community was retrieved directly from the nucleic acid extracts, while the DNA of the active soil microbial community was separated after bromodeoxyuridine labeling. Bacterial 16S rRNA gene and fungal internal transcribed spacer region gene-based amplicon sequencing was carried out for both active and total, while gene copy numbers were quantified only for the total soil microbial community. Additionally, soil respiration and physico-chemical parameters were analyzed to investigate overall soil microbial activity. The bacterial and fungal gene copy numbers were significantly affected by single biocides and combined biocide soil treatment but not soil respiration and physico-chemical parameters. While the total soil microbiome experienced only minor effects from single and combined biocide treatment, the active soil microbiome was significantly impacted in its diversity, richness, composition, and functional patterns. The active bacterial richness was more sensitive than fungal richness. However, the adverse effects of the biocide combination treatments on soil bacterial richness were highly dependent on the identities of the biocide combination. Our results demonstrate that the presence of biocides frequently used in building materials affects the active soil microbiome. Thereby, the approach described herein can be used as an ecotoxicological measure for the effect on complex soil environments in future studies.

Application, release, ecotoxicological assessment of biocide in building materials and its soil microbial response.

Biocides are used in building materials to protect the building against microbial colonization and biodeterioration. However, these biocides are introduced by gradual leaching into soils in proximity of the buildings. This review discusses the aspects and characteristics of biocides from building materials in terms of (i) in-situ leaching and simulation thereof in-vitro and in-field tests, (ii) persistence, as well as photolytic and biodegradation, and its influence on toxicological evaluation, and (iii) evaluation of terrestrial toxicity by conventional ecotoxicological tests and novel holistic testing approaches. These aspects are influenced by multiple parameters, out of which water availability, physicochemical properties of microhabitats, combination of biocidal building materials, soil parameters, and composition of the soil microbiome are of utmost relevance. Deeper understanding of this multiparametric system and development of comprehensive characterization methodologies remains crucial, as to facilitate realistic assessment of the environmental impact of biocides used in construction materials and the corresponding degradation byproducts.