Allogeneic pASC transplantation in humanized pigs attenuates cardiac remodeling post-myocardial infarction.

Cell therapy repair strategies using adult mesenchymal stromal cells have shown promising evidence to prevent cardiac deterioration in rodents even in the absence of robust differentiation of the cells into cardiomyocytes. We tested whether increasing doses of porcine adipose-tissue derived mesenchymal stem cells (pASCs) increase cardiac tissue perfusion in pigs post-myocardial infarction (MI) receiving angiotensin-converting-enzyme inhibitor (ACE inhibitors) and Beta-blockers similarly to patients. Female pigs were subjected to MI induction by sponge permanent occlusion of left circumflex coronary artery (LCx) generating approximately 10% of injured LV area with minimum hemodynamic impact. We assessed tissue perfusion by real time myocardial perfusion echocardiography (RTMPE) using commercial microbubbles before and following pASCs treatment. Four weeks after the occlusion of the left circumflex artery, we transplanted placebo or pASCs (1, 2 and 4x106 cells/Kg BW) into the myocardium. The highest dose of pASCs increased myocardial vessel number and blood flow in the border (56% and 3.7-fold, respectively) and in the remote area (54% and 3.9-fold, respectively) while the non-perfused scar area decreased (up to 38%). We also found an increase of immature collagen fibers, although the increase in total tissue collagen and types I and III was similar in all groups. Our results provide evidence that pASCs-induced stimulation of tissue perfusion and accumulation of immature collagen fibers attenuates adverse remodeling post-MI beyond the normal beneficial effects associated with ACE inhibition and beta-blockade.

Pesquisa de coliformes totais e termotolerantes em mãos de manipuladores da merenda escolar da rede municipal de ensino de Marialva, PR / Research of total and thermotolerant coliforms in the hands of school lunch handlers of the municipal school network of Marialva, PR

A alimentação dentro dos padrões higiênicos satisfatórios é essencial para a saúde, sendo que uma redução na fiscalização pode resultar em ocorrência de doenças veiculadas por alimentos (DVA's). A prevalência de bactérias indicadoras de contato com fezes nas mãos pode ser usada no monitoramento do comportamento dos manipuladores de alimentos em relação à higiene. A fim de avaliar as condições de fabricação da merenda escolar, foi realizada uma pesquisa identificando-se a presença de coliformes nas mãos destes profissionais, em escolas da rede municipal de ensino de Marialva - Paraná. No presente estudo foram avaliadas 24 amostras de lavagem de mãos, das quais 23 (95,83%) apresentaram- -se positivas para coliformes totais e 14 (58,33%) para coliformes termotolerantes. Através da aplicação de um checklist foi possível constatar hábitos não higiênicos, levando a concluir que um maior controle sanitário de pessoal deve ser empregado.

Pesquisa de coliformes totais e termotolerantes em mãos de manipuladores da merenda escolar da rede municipal de ensino de Marialva - PR

A alimentação dentro dos padrões higiênicos satisfatórios é essencial para a saúde, sendo que uma redução na fiscalização pode resultar em ocorrência de doenças veiculadas por alimentos (DVA's). A prevalência de bactérias indicadoras de contato com fezes nas mãos pode ser usada no monitoramento do comportamento dos manipuladores de alimentos em relação à higiene. A fim de avaliar as condições de fabricação da merenda escolar, foi realizada uma pesquisa identificando-se a presença de coliformes nas mãos destes profissionais, em escolas da rede municipal de ensino de Marialva - Paraná. No presente estudo foram avaliadas 24 amostras de lavagem de mãos, das quais 23 (95,83%) apresentaram- -se positivas para coliformes totais e 14 (58,33%) para coliformes termotolerantes. Através da aplicação de um checklist foi possível constatar hábitos não higiênicos, levando a concluir que um maior controle sanitário de pessoal deve ser empregado.(AU)

The alimentation following satisfactory hygienic pattern is essential for health, but if there is a reduction of inspection it may result in diseases related to aliments. The prevalence of bacteria indicated by contact with excrement found in people' s hands may be used to monitor the behavior of people who are dealing with aliments regarding to hygiene. Aiming I at evaluating the conditions of manufacturing food for students, it was made a research identifying the presence of coliforms in such professionals' hands, at municipal schools, in Marialva/PR. /n the present study 24 samples of washing hands have been analyzed, and 23 of them (95.83%) showed themselves positive for total coliforms and /4 (58,33%) for thermo tolerant coliforms. A checklist was applied and it was possible to verify non hygienic habits, concluding that a wider sanitary control for people involved in such activity must be done.(AU)

Catheter-based induction of renal ischemia/reperfusion in swine: description of an experimental model.

Several techniques to induce renal ischemia have been proposed: clamp, PVA particles, and catheter-balloon. We report the development of a controlled, single-insult model of unilateral renal ischemia/reperfusion (I/R) without contralateral nephrectomy, using a suitable model, the pig. This is a balloon-catheter-based model using a percutaneous, interventional radiology procedure. One angioplasty balloon-catheter was placed into the right renal artery and inflated for 120 min and reperfusion over 24 h. Serial serums were sampled from the inferior vena cava and urine was directly sampled from the bladder throughout the experiment, and both kidneys were excised after 24 h of reperfusion. Analyses of renal structure and function were performed by hematoxylin-eosin/periodic Acid-Schiff, serum creatinine (SCr), blood urea nitrogen (BUN), fractional excretion of ions, and glucose, SDS-PAGE analysis of urinary proteins, and serum neutrophil gelatinase-associated lipocalin (NGAL). Total nitrated protein was quantified to characterize oxidative stress. Acute tubular necrosis (ATN) was identified in every animal, but only two animals showed levels of SCr above 150% of baseline values. As expected, I/R increased SCr and BUN. Fractional sodium, potassium, chloride, and bicarbonate excretion were modulated during ischemia. Serum-nitrated proteins and NGAL had two profiles: decreased with ischemia and increased after reperfusion. This decline was associated with increased protein excretion during ischemia and early reperfusion. Altogether, these data show that the renal I/R model can be performed by percutaneous approach in the swine model. This is a suitable translational model to study new early renal ischemic biomarkers and pathophysiological mechanisms in renal ischemia.