Color stability, surface, and physicochemical properties of three-dimensional printed denture base resin reinforced with different nanofillers.

Various materials have been introduced for the three-dimensional (3D) printing of dentures. In this study, the color stability and surface and physicochemical properties of 3D-printed denture base resins with four types of nanofiller particles were evaluated. Al2O3, ZnO, CeZr, and SiO2 nanofillers were added to a 3D printable denture base-resin matrix and subjected to digital light processing. The specimens were immersed in Coke, coffee, black tea, or distilled water for 6 days. For the assessment of color differences, 6 samples were analyzed using a spectrophotometer. In a separate investigation, surface properties of 10 samples were examined, while a different set of 6 samples was used to analyze water sorption and solubility. All experimental groups exhibited higher color stability in Coke than the control group. However, the groups containing ZnO and CeZr had lower color stability in coffee and black tea than the control group. Moreover, they had agglomerated nanofillers and lower gloss than the control group. Compared with that of the control group, the contact angle of the CeZr group and microhardness of the ZnO group were not significantly different. Water sorption was higher in the Al2O3 group, whereas the solubility of the experimental and control groups was not statistically significant. The results demonstrated the significant effect of ZnO and CeZr nanofillers on the color stability of the dentures when exposed to discoloring beverages. These results will facilitate the development of fillers that enhance the resistance of 3D printed denture base resins to discoloration in the oral environment.

CD4+ cytotoxic T cells: an emerging effector arm of anti-tumor immunity.

While CD8+ cytotoxic T cells have long been considered the primary effector in controlling tumors, the involvement of CD4+ "helper" T cells in anti-tumor immunity has been underappreciated. The investigations of intra-tumoral T cells, fueled by the recent advances in genomic technologies, have led to a rethinking of the indirect role of CD4+ T cells that have traditionally been described as a "helper". Accumulating evidence from preclinical and clinical studies indicates that CD4+ T cells can acquire intrinsic cytotoxic properties and directly kill various types of tumor cells in a major histocompatibility complex class II (MHC-II)-dependent manner, as opposed to the indirect "helper" function, thus underscoring a potentially critical contribution of CD4+ cytotoxic T cells to immune responses against a wide range of tumor types. Here, we discuss the biological properties of anti-tumor CD4+ T cells with cytotoxic capability and highlight the emerging observations suggesting their more significant role in anti-tumor immunity than previously appreciated. [BMB Reports 2023; 56(3): 140-144].

Significant paradigm of beauty ecosystem after COVID-19 pandemic in Republic of Korea.

BACKGROUND: The beauty industry, one of the face-to-face the essential industries in the COVID-19 pandemic, has been hit hard. AIMS: We present the current state of the beauty ecosystem in the corona pandemic and the future direction of the beauty industry after COVID-19. METHODS: This review paper is a literature review, and a descriptive review approach was used in this study. A total of 100-150 references were selected using representative journal search sites such as PubMed, Google Scholar, Scopers, Research Gate, and RISS, and a total of 24 papers were finally selected as of 2017-2022 using PRISMA flow diagram. RESULTS: In Korea, the beauty industry, which is essential to face-to-face due to COVID-19, was hit hard. The contraction of consumer sentiment led to a drop in sales. The online market has become more active as offline activities have declined sharply. Even during the COVID-19 era, the Republic of Korea is an IT powerhouse. With the widespread use of K-drama and K-pop, the interest in Korean beauty and fashion has increased rapidly throughout the world. Therefore, K-Makeup, hair, skincare, and nailcare are all the rage through YouTube. Predict new changes in the Korean beauty industry market, which is the center of K-beauty and K-cosmetics. As a leader in the beauty industry, the Republic of Korea will present the sustainability of the beauty ecosystem and the direction of its development.

Mitogen-Activated Protein Kinase CaDIMK1 Functions as a Positive Regulator of Drought Stress Response and Abscisic Acid Signaling in Capsicum annuum.

Protein phosphorylation by kinase is an important mechanism for adapting to drought stress conditions. Here, we isolated the CaDIMK1 (Capsicum annuum drought-induced MAP kinase 1) from dehydrated pepper leaf tissue and functionally characterized it. Subcellular localization analysis revealed that the CaDIMK1 protein was localized in the cytoplasm and nucleus. CaDIMK1-silenced pepper plants exhibited drought-susceptible phenotypes that were characterized by increased transpiration rates, low leaf temperatures, and decreased stomatal closure. In contrast, CaDIMK1-overexpressing (OX) transgenic Arabidopsis plants were hypersensitive to abscisic acid (ABA) from germination to adult growth stages. Furthermore, the CaDIMK1-OX plants were tolerant to drought stress. The transcript levels of several stress-related genes were high in CaDIMK1-OX plants than in wild-type plants. Taken together, our data demonstrate that CaDIMK1 acts as a positive modulator of drought tolerance and ABA signal transduction in pepper plants.

Phase I and pharmacokinetic study of the vascular-disrupting agent CKD-516 (NOV120401) in patients with refractory solid tumors.

We report a phase I pharmacological study of an oral formulation of CKD-516, a vascular-disrupting agent, in patients with refractory solid tumors. Twenty-seven patients (16 in the dose-escalation cohort and 11 in the expansion cohort) received a single daily dose (5-25 mg) of CKD-516 five days per week. Nausea (67%) and diarrhea (63%) were the most common treatment-related adverse events. The recommended phase II dose of oral CKD-516 was 20 mg/d (15 mg/d with a body surface area (BSA) <1.65 m2 ). Notably, S-516 half-lives in patients receiving 15-20 mg CKD-516/d significantly differed between patients with and without splenomegaly that is suggestive of portal hypertension associated with liver cirrhosis (6.1 vs 4.6 hours, respectively). Of 11 patients without splenomegaly who completed at least one cycle of a daily CKD-516 dose of either 15 or 20 mg, only one patient (9.1%) suffered from any dose-limiting toxicity. We conclude that a daily oral dose of 15 or 20 mg CKD-516 five days per week could be tolerable in patients without liver cirrhosis.

Cellular Behavior of RAW264.7 Cells in 3D Poly(ethylene glycol) Hydrogel Niches.

Although macrophages undergo dynamic cellular responses in diverse extracellular environments, macrophage research has mostly relied on conventional culture methodologies such as two-dimensional and suspension cultures. In contrast, recent efforts have revealed evidence of the characteristic cellular behaviors of macrophages in actual tissues using a three-dimensional (3D) culture matrix. In this work, we exploited a poly(ethylene glycol)-based hydrogel as a macrophage culture matrix and observed cellular behaviors in 3D by manipulating the matrix properties. In the 3D microenvironment, macrophage-like RAW264.7 cells proliferated and formed spherical clusters by degrading the surrounding hydrogel network. Interestingly, we observed the significant upregulation of matrix metalloproteinases (MMPs) (i.e., MMP9 and MMP14) as well as M1 polarization markers (i.e., iNOS, COX2, TNF-α) in 3D, whereas M2 polarization markers (i.e., CD206, Arg1, TGF-ß) were downregulated. Specifically, the expressions of both M1 and M2 markers were simultaneously increased in a stiff matrix compared to those of a soft matrix. In addition, matrix degradability significantly influenced the TNF-α secretion of encapsulated RAW264.7 cells. The MMP sensitivity of the hydrogel decreased TNF-α expression in a soft matrix, whereas it upregulated TNF-α in a stiff matrix compared to those of MMP-insensitive hydrogel. These findings suggest that the highly tunable poly(ethylene glycol) hydrogels can dictate macrophage behavior by altering the surrounding 3D microenvironment.

ß-Thujaplicin modulates estrogen receptor signaling and inhibits proliferation of human breast cancer cells.

ß-Thujaplicin, one of the major constituents in Chamaecyparis obtusa, has been demonstrated to exert different health beneficial efficacy, but the role of ß-thujaplicin in regulating mammary tumorigenesis has not been investigated. In this study, we found that ß-thujaplicin significantly suppressed the proliferation through arresting the cell cycle transition from G1 to S phase as well as inhibited the expression of cell cycle-related proteins, cyclin D1, and cyclin-dependent kinase 4 (CDK4) in MCF-7 and T47D luminal subtype breast cancer cells. In addition, estrogen receptor α (ER-α) was down-regulated by ß-thujaplicin via enhanced proteolysis by ubiquitination, which led to cell growth inhibition. These results suggest that ß-thujaplicin may be considered as a potent agent regulating the hormone sensitive mammary tumorigenesis.