ABSTRACT
Influenza is a significant cause of morbidity and mortality worldwide. Here we show changes in the abundance and activation states of more than 50 immune cell subsets in 35 individuals over 11 time points during human A/California/2009 (H1N1) virus challenge monitored using mass cytometry along with other clinical assessments. Peak change in monocyte, B cell, and T cell subset frequencies coincided with peak virus shedding, followed by marked activation of T and NK cells. Results led to the identification of CD38 as a critical regulator of plasmacytoid dendritic cell function in response to influenza virus. Machine learning using study-derived clinical parameters and single-cell data effectively classified and predicted susceptibility to infection. The coordinated immune cell dynamics defined in this study provide a framework for identifying novel correlates of protection in the evaluation of future influenza therapeutics.
Subject(s)
Influenza A Virus, H1N1 Subtype/immunology , Influenza, Human/immunology , Killer Cells, Natural/immunology , Lymphocyte Activation , T-Lymphocytes/immunology , Female , Humans , MaleABSTRACT
False-positive (FP) errors during recognition memory testing often interfere with interpretation of Wada test results. This study examined which clinical and neuropsychological variables provide the best prediction of these errors. Fifty-six patients completed the Wada test and the California Verbal Learning Test (CVLT). Subjects with three or more FP errors on the recognition trials of the CVLT were nearly twice as likely to exhibit FP responding during the Wada test. Further analysis indicates that FP errors during the Wada test appear to be the primary result of a stable and liberal response-bias rather than a result of any other neurological or procedure-related factor.
Subject(s)
Amobarbital/pharmacology , Hypnotics and Sedatives/pharmacology , Neuropsychological Tests/statistics & numerical data , Recognition, Psychology/drug effects , Adult , Dose-Response Relationship, Drug , Electroencephalography/methods , False Positive Reactions , Female , Humans , Language , Male , Predictive Value of Tests , Recognition, Psychology/physiology , Regression Analysis , Verbal Learning/drug effects , Verbal Learning/physiologyABSTRACT
As apófises são núcleos de ossificação secundários que se desenvolvem com o crescimento. Estão sujeitas a forças de tração porque servem para inserção de músculos e ligamentos. Se a força de tração torna-se maior em magnitude e freqüência, pode ocorrer irritação da fase, resultando na apofisite. As lesões apofisárias ocorrem em adolescentes, geralmente presentes com dor articu-lar, associadas com o crescimento, imaturidade esquelética, microtraumas repetidos e imaturidade músculo-tendínea. As apofisites e as suas localizações principais são: doença de Osgood-Schlatter (tuberosidade tibial), apofisites do quadril (crista ilíaca e tuberosidade isquiática), doença de Sever (calcâneo posterior), apofisite do navicular, síndrome de Sindig-Larsen-Johans-son (patela inferior) e doença de lselin (tuberosidade da base do quinto metatarsal). 0 principal objetivo será demonstrar os principais achados de imagem destas afecções pela ressonância magnética e raios-X, bem como discutir os principais diagnósticos diferenciais.
