Heterologous Expression of OtsB Increases Tuber Yield and Phenotypic Stability in Potato under Both Abiotic and Biotic Stresses.

Climate-smart and sustainable crops are needed for the future. Engineering crops for tolerance of both abiotic and biotic stress is one approach. The accumulation of trehalose, controlled through trehalose-6-phosphate synthase (TPS) or OtsA and trehalose-6-phosphate phosphatase (TPP) or OtsB genes in microbes, is known to provide protection for many microbial and fungal species against abiotic stress. The effect of trehalose accumulation in plant species is less understood. Here, we studied the heterologous expression of Escherichia coli OtsB in potato (Solanum tuberosum var. 'Desiree') with regards to stress tolerance. The performance of transgenic lines was assessed in both growth chambers and greenhouse mesocosms. Overexpressing potato OtsB lines significantly increased resilience to heat, photoperiod, herbivory, and competition when compared with wildtype plants. Most strikingly, when subjected to high temperatures, transgenic lines exhibited a significantly lower reduction in tuber yield ranging from 40% to 77%, while wildtype plants experienced a 95% decrease in tuber yield. When exposed to competitors in a selected StSP3D::OtsB line, tuber yield was 1.6 times higher than wildtype. Furthermore, transgenic lines performed significantly better under low-nutrient regimes: under competition, yield increased by 1.5-fold. Together, these results demonstrate that increased trehalose has the potential to create more resistant and stable crop plants.

Profiles of parents' emotion socialization within a multinational sample of parents.

Introduction: Seminal emotion socialization theories classify parents according to two patterns of parent emotion socialization processes: 'emotion coaching' (i.e., parents validate and teach children about emotions) versus 'emotion dismissing' parenting (i.e., parents minimize and dismiss their children's emotions). However, empirical evidence supporting this binary distinction of parents remains limited. Our objective was to investigate whether parents can be differentiated by distinct patterns in their (1) beliefs about children's emotions, (2) emotion regulation, and (3) emotion-related parenting practices. Method: Participants were parents of children aged 4-10 years from the Child and Parent Emotion Study (N = 869) (https://bmjopen.bmj.com/content/10/10/e038124). Parents completed self-reported measures of emotion socialization processes via an online survey, which took 20-30 min to complete. Data included in the current study were collected May-August 2019. We conducted a latent profile analysis of parents' emotion socialization (13 indicators). To assess reliability of the profiles, we examined stability of the profiles across (1) parents of children in early versus middle childhood, and (2) fathers versus mothers, via measurement invariance testing. Further, to assess for construct validity of the profiles, we examined concurrent associations between six criterion constructs and parents' emotion socialization profiles. Results: A three-profile model emerged characterizing parents by: (1) emotion coaching; (2) emotion dismissing; (3) emotion disengaged. There was strong support for construct validity and reliability. Discussion: Our study provides empirical support for distinct differentiated classifications of emotion coaching and emotion dismissing parenting, aligned with emotion socialization theories. We further extend on extant theory and suggest a third 'emotion disengaged' classification, describing parents with moderate levels of emotion dismissing parenting and low levels of emotion coaching parenting. It should be noted that the profiles were derived with self-report data, therefore, data may have been biased by contextual factors. Furthermore, the study sample consisted of Western families from affluent backgrounds. The field should focus efforts on conducting person-centered studies with more diverse samples in future.

Social and emotional determinants of parental reflective functioning in a multinational sample.

Parental reflective functioning refers to parents' capacity to consider their child's internal experiences and is associated with secure parent-child attachment, sensitive parenting behavior, and positive child socioemotional development. However, research into determinants of parental reflective functioning in large diverse samples has been scarce. Therefore, using a large multinational sample and longitudinal design, we aimed to: (a) identify sociodemographic determinants of parental reflective functioning; (b) investigate whether parental emotion regulation is a psychological determinant of parental reflective functioning; and (c) assess whether child negative affect, parent stress, and child age moderate longitudinal associations between parent emotion regulation and parental reflective functioning. Data were two time points of the Child and Parent Emotion Study following 2,208 parents (68% female) of a child aged 0-10 years residing in English-speaking countries. Parent emotion dysregulation, parent male cisgender status, speaking a language other than English, younger parent age, and older child age were associated with lower parental reflective functioning 12 months later, as indicated by higher levels of prementalizing. Child negative affect moderated the association between parent emotion dysregulation and parental reflective functioning, whereas child age and parent stress did not. Specifically, parent difficulty controlling impulses when distressed was associated with higher levels of prementalizing modes of reflective functioning only when child negative affect was moderate-to-high. The interplay between parent emotion dysregulation and child negative affect highlights the importance of considering both parent and child characteristics in intervention planning and suggests that personalizing parenting support according to these characteristics may be beneficial. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Creation of a Short-Form and Brief Short-Form Version of the Coping With Children's Negative Emotions Scale.

The Coping with Children's Negative Emotions Scale (CCNES) is a widely used measure of parent emotion socialization; however, it is a lengthy measure and it is unclear whether all items are appropriately aligned with, and fully capture, the underlying constructs. We aimed to examine content validity of the CCNES, evaluate the theoretical alignment between the CCNES and Gottman, Katz and Hooven's meta-emotion theory, and develop two short-forms. Participants were parents of children aged 4 to 10 years (N = 937) from the longitudinal study the Child and Parent Emotion Study (https://bmjopen.bmj.com/content/10/10/e038124). Content experts qualitatively evaluated parent-report items of the CCNES and additional items that measured empathy. Nineteen of the 84 items were found to not align with the meta-emotion theory. The latent structures of the CCNES and empathy subscales were quantitatively evaluated via confirmatory factor analysis. Items with poor psychometric properties were subsequently removed. An 18-item short-form (three emotion coaching subscales, three emotion dismissing subscales) and 6-item brief short-form (one emotion coaching subscale, one emotion dismissing subscale) with strong psychometric properties were created using a calibration sample (n = 468, that is, 50% of N = 937) and cross-validated with a validation sample. The short-form CCNES measures provide viable, theoretically consistent alternatives to the original CCNES measure.

Human wildtype tau expression in cholinergic pedunculopontine tegmental neurons is sufficient to produce PSP-like behavioural deficits and neuropathology.

Progressive Supranuclear Palsy (PSP) is the most common atypical parkinsonism and exhibits hallmark symptomology including motor function impairment and dysexecutive dementia. In contrast to Parkinson's disease, the underlying pathology displays aggregation of the protein tau, which is also seen in disorders such as Alzheimer's disease. Currently, there are no pharmacological treatments for PSP, and drug discovery efforts are hindered by the lack of an animal model specific to PSP. Based on previous results and clinical pathology, it was hypothesized that viral deposition of tau in cholinergic neurons within the hindbrain would produce a tauopathy along neural connections to produce PSP-like symptomology and pathology. By using a combination of ChAT-CRE rats and CRE-dependent AAV vectors, wildtype human tau (the PSP-relevant 1N4R isoform; hTau) was expressed in hindbrain cholinergic neurons. Compared to control subjects (GFP), rats with tau expression displayed deficits in a variety of behavioural paradigms: acoustic startle reflex, marble burying, horizontal ladder and hindlimb motor reflex. Postmortem, the hTau rats had significantly reduced number of cholinergic pedunculopontine tegmentum and dopaminergic substantia nigra neurons, as well as abnormal tau deposits. This preclinical model has multiple points of convergence with the clinical features of PSP, some of which distinguish between PSP and Parkinson's disease.

The Child and Parent Emotion Study: protocol for a longitudinal study of parent emotion socialisation and child socioemotional development.

INTRODUCTION: Parents shape child emotional competence and mental health via their beliefs about children's emotions, emotion-related parenting, the emotional climate of the family and by modelling emotion regulation skills. However, much of the research evidence to date has been based on small samples with mothers of primary school-aged children. Further research is needed to elucidate the direction and timing of associations for mothers and fathers/partners across different stages of child development. The Child and Parent Emotion Study (CAPES) aims to examine longitudinal associations between parent emotion socialisation, child emotion regulation and socioemotional adjustment at four time points from pregnancy to age 12 years. CAPES will investigate the moderating role of parent gender, child temperament and gender, and family background. METHODS AND ANALYSIS: CAPES recruited 2063 current parents from six English-speaking countries of a child 0-9 years and 273 prospective parents (ie, women/their partners pregnant with their first child) in 2018-2019. Participants will complete a 20-30 min online survey at four time points 12 months apart, to be completed in December 2022. Measures include validated parent-report tools assessing parent emotion socialisation (ie, parent beliefs, the family emotional climate, supportive parenting and parent emotion regulation) and age-sensitive measures of child outcomes (ie, emotion regulation and socioemotional adjustment). Analyses will use mixed-effects regression to simultaneously assess associations over three time-point transitions (ie, T1 to T2; T2 to T3; T3 to T4), with exposure variables lagged to estimate how past factors predict outcomes 12 months later. ETHICS AND DISSEMINATION: Ethics approval was granted by the Deakin University Human Research Ethics Committee and the Deakin University Faculty of Health Human Research Ethics Committee. We will disseminate results through conferences and open access publications. We will invite parent end users to co-develop our dissemination strategy, and discuss the interpretation of key findings prior to publication. TRIAL REGISTERATION: Protocol pre-registration: DOI 10.17605/OSF.IO/NGWUY.