ABSTRACT
As part of the seasonal mosquito control activities in the city of Pécs (Baranya County, Hungary), a total of 1123 adult female mosquitoes belonging to 18 species (including the invasive species Aedes koreicus) were collected from human-inhabited areas, using CO2-baited traps, during two consecutive years. To survey the presence and prevalence of filarial parasites in these mosquitoes, we performed a molecular survey for filarial DNA, attempted by PCR using generic primers (COI), and followed by DNA sequencing. Filaroid nematode DNA was detected in 4% of investigated mosquito pools. Out of 410 pools, 9 pools of mosquitoes were positive for Dirofilaria repens (Aedes vexans, Aedes koreicus, Coquillettidia richiardii), and/or Dirofilaria immitis (Ae. vexans, Cq. richiardii), and further 8 pools were positive for Setaria tundra (Ae. vexans, Cq. richiardii). Our study provides novel insight for prevalence of filaroid nematodes in mosquitoes occurring in close proximity to humans, thereby highlights the possible human and veterinary health importance of these mosquito species, including the recently introduced invasive mosquito Ae. koreicus.
Subject(s)
Dirofilariasis/parasitology , Mosquito Vectors/parasitology , Spirurida/isolation & purification , Aedes/parasitology , Animals , Dirofilaria immitis/classification , Dirofilaria immitis/genetics , Dirofilaria immitis/isolation & purification , Dirofilaria immitis/physiology , Dirofilaria repens/classification , Dirofilaria repens/genetics , Dirofilaria repens/isolation & purification , Dirofilaria repens/physiology , Dirofilariasis/epidemiology , Dirofilariasis/transmission , Ecosystem , Female , Humans , Hungary/epidemiology , Male , Mosquito Control , Mosquito Vectors/classification , Mosquito Vectors/physiology , Setaria Nematode/classification , Setaria Nematode/genetics , Setaria Nematode/isolation & purification , Setaria Nematode/physiology , Spirurida/classification , Spirurida/genetics , Spirurida/physiology , Urban HealthABSTRACT
In June 2016, three adult females of Aedes koreicus mosquitoes were trapped in the urban area of Pécs, Southwest Hungary. The introduction of this invasive mosquito species in this region, along with the recent detection in Germany, may indicate the capability of the species to spread across Europe. Along with Aedes albopictus and Aedes japonicus mosquitoes, this is the third invasive mosquito species occurred in Hungary.