ABSTRACT
The genus Sarcocystis is an abundant group of Apicomplexa parasites found in mammals, birds, and reptiles. These parasites are characterised by the formation of sarcocysts in the muscles of intermediate hosts and the development of sporocysts in the intestines of definitive hosts. The identification of Sarcocystis spp. is usually carried out in carcasses of animals, while there is a lack of studies on the detection of Sarcocystis species in blood samples. In the current study, blood samples of 214 yellow-necked mice (Apodemus flavicollis) and 143 bank voles (Clethrionomys glareolus) from Lithuania were examined for Sarcocystis. The molecular identification of Sarcocystis was carried out using nested PCR of cox1 and 28S rRNA and subsequent sequencing. Sarcocystis spp. were statistically (p < 0.01) more frequently detected in the bank vole (6.3%) than in yellow-necked mice (0.9%). The analysed parasites were observed in four different habitats, such as mature deciduous forest, bog, natural meadow, and arable land. Three species, Sarcocystis funereus, Sarcocystis myodes, and Sarcocystis cf. glareoli were confirmed in the bank vole, whereas only Sarcocystis myodes were found in yellow-necked mice. The obtained results are important in the development of molecular identification of Sarcocystis parasites in live animals.
ABSTRACT
Family Laelapidae is an ecologically diverse group that includes free-living species and parasites of vertebrates and invertebrates. At least seven genera in this family are associated with small mammals. In this study, ectoparasitic laelapid mites of rodents and shrews were investigated in Lithuania. In total, 2,274 small mammal specimens of 12 species were trapped and 6,089 laelapid mites were collected. The updated list of ectoparasitic mites in Lithuania included 21 mite species. Seven mite species were identified as highly specific for a host species or genus, one species was moderately specific, and four mite species were assigned to generalist parasites. All host species had one or two superdominant mite species. The prevalence and mean intensity varied significantly depending on host species and habitat. We analyzed the influence of the host (species, sex, age) and environmental factors (landscape morphology type, habitat, anthropogenic effect) on the abundance of the mite community and most numerous mite species, as well as the impact of the host community (Shannon's diversity index, species richness, host abundance) on mean abundance of the mite community. Only particular host species (Apodemus flavicollis, Microtus agrestis, and Microtus arvalis) and habitats (pastures, mixed forests) influenced the abundance of mites.
Subject(s)
Mites , Animals , Lithuania , Mammals/parasitology , Arvicolinae/parasitology , Murinae , ShrewsABSTRACT
Wild rodents, as natural reservoir hosts carrying various species of pathogens, play an important role in the evolution and emergence of zoonotic diseases. In this study, protist parasites, namely Babesia sp., Trypanosoma sp. and Hepatozoon sp. were studied in rodent populations in Lithuania. Two hundred forty rodent specimens of seven species were analysed by a combined approach using polymerase chain reaction (PCR)-based techniques and traditional microscopic examination. The total prevalence of blood parasites reached 35% in rodent communities. The prevalence of Hepatozoon sp. reached the highest value (32%), followed by Trypanosoma sp. (5%) and Babesia sp. (3%). Myodes glareolus and Microtus agrestis were the most heavily infected rodent species. Comparison of microscopy and PCR-based methods showed that the two approaches might give different results and thus can lead to an underestimation of the actual prevalence and abundance of parasites. In our study, PCR-based assays were more sensitive and robust than traditional microscopy. However, precise molecular results for the estimation of the prevalence of Babesia sp. and Hepatozoon sp. were achieved only by using several sets of primers. To avoid inaccurate results, the improvement and detailed description of molecular and microscopy protocols are required.