ABSTRACT
Standard residual pesticides applied to US military materials such as camouflage netting can reduce mosquito biting pressure in the field but may contribute to the evolution of resistance. However, residual applications of a spatial repellent such as transfluthrin could allow mosquitoes the opportunity to escape, only inducing mortality if insects linger, for example after becoming trapped in a treated tent. In this study we investigated the capability of transfluthrin on 2 types of US military material to reduce natural populations of disease vector mosquitoes in a cool-arid desert field environment in southern California. We found that transfluthrin could reduce Culex tarsalis incursion into protected areas by up to 100% upon initial treatment and up to 45% for at least 16 days posttreatment, showing that this compound could be an effective element in the US Department of Defense integrated vector management system appropriate for further study.
Subject(s)
Culex , Cyclopropanes , Fluorobenzenes , Insecticides , Mosquito Control , Animals , California , Desert Climate , Equipment and Supplies , Military FacilitiesABSTRACT
We investigated the capability of transfluthrin on US military camouflage netting to reduce collections of tabanid biting flies in a warm-temperate field environment on the Gulf Coast of Florida. We found that transfluthrin significantly reduced collections of a variety of medically and veterinarily important tabanids inside protected areas by up to 96% upon initial treatment and up to 74% after 20 days posttreatment. These results suggest that transfluthrin could be an effective element in the US Department of Defense integrated pest management system and leveraged in civilian scenarios to protect livestock and humans from potential mechanical transmission of pathogens and disruption of activities caused by painful bites.
Subject(s)
Cyclopropanes , Diptera , Fluorobenzenes , Insect Control , Insect Repellents , Military Personnel/statistics & numerical data , Animals , Environment , FloridaABSTRACT
Efficacies of a handheld thermal fogger (Patriot™) and a backpack ultra-low volume (ULV) sprayer (Twister™) with combinations of 2 different adulticides (pyrethrin, deltamethrin) and an insect growth regulator (pyriproxyfen) were field-tested and compared for their impact on reducing indoor Aedes aegypti populations in Thailand. The effectiveness of the indoor space sprays was evaluated by sampling the natural Ae. aegypti population in houses and determining their physiological status, by monitoring mortality of sentinel caged mosquitoes (AFRIMS strain) and by assessing larval mortality in laboratory bioassays using water exposed to the spray. A total of 14,742 Ae. aegypti were collected from Biogents Sentinel traps in this study. The combination of ULD® BP-300 (3% pyrethrin) and NyGuard® (10% pyriproxyfen) sprayed either by the Patriot or Twister significantly reduced some Ae. aegypti populations up to 20 days postspray relative to the control clusters. The addition of pyriproxyfen to the adulticide extended how long household mosquito populations were suppressed. In 2 of the 4 products being compared, the Twister resulted in higher mortality of caged mosquitoes compared with the Patriot. However, neither machine was able to achieve high mortality among Ae. aegypti placed in hidden (protected) cages. The larval bioassay results demonstrated that the Twister ULV provided better adult emergence inhibition than the Patriot (thermal fogger), likely due to larger droplet size.
Subject(s)
Aedes , Insecticides , Juvenile Hormones , Mosquito Control , Nitriles , Pyrethrins , Pyridines , Animals , ThailandABSTRACT
Capture rates of insectary-reared female Aedes albopictus (Skuse), Anopheles quadrimaculatus Say, Culex nigripalpus Theobald, Culex quinquefasciatus Say and Aedes triseriatus (Say) in CDC-type light traps (LT) supplemented with CO2 and using the human landing (HL) collection method were observed in matched-pair experiments in outdoor screened enclosures. Mosquito responses were compared on a catch-per-unit-effort basis using regression analysis with LT and HL as the dependent and independent variables, respectively. The average number of mosquitoes captured in 1âmin by LT over a 24-h period was significantly related to the average number captured in 1âmin by HL only for Cx. nigripalpus and Cx. quinquefasciatus. Patterns of diel activity indicated by a comparison of the mean response to LT and HL at eight different times in a 24-h period were not superposable for any species. The capture rate efficiency of LT when compared with HL was ≤15% for all mosquitoes except Cx. quinquefasciatus (43%). Statistical models of the relationship between mosquito responses to each collection method indicate that, except for Ae. albopictus, LT and HL capture rates are significantly related only during certain times of the diel period. Estimates of mosquito activity based on observations made between sunset and sunrise were most precise in this regard for An. quadrimaculatus and Cx. nigripalpus, as were those between sunrise and sunset for Cx. quinquefasciatus and Ae. triseriatus.
Subject(s)
Culicidae/physiology , Mosquito Control/methods , Specimen Handling/methods , Animals , Carbon Dioxide , Female , Humans , Light , Models, Statistical , Mosquito Control/instrumentation , Regression Analysis , Species SpecificityABSTRACT
Four types of commercial mosquito control traps, the Mosquito Magnet Pro (MMP), the Sentinel 360 (S360), the BG-Sentinel (BGS), and the Mega-Catch Ultra (MCU), were compared with a standard Centers for Disease Control and Prevention (CDC) light trap for efficacy in collecting phlebotomine sand flies (Diptera: Psychodidae) in a small farming village in the Nile River Valley 10 km north of Aswan, Egypt. Each trap was baited with either carbon dioxide (CO2) from combustion of butane gas (MMP), dry ice (CDC and BGS traps), light (MCU and S360), or dry ice and light (CDC). Traps were rotated through five sites in a5 x 5 Latin square design, repeated four times during the height of the sand fly season (June, August, and September 2007) at a site where 94% of sand flies in past collections were Phlebotomus papatasi (Scopoli). A total of 6,440 sand flies was collected, of which 6,037 (93.7%) were P. papatasi. Of the CO2-baited traps, the BGS trap collected twice as many P. papatasi as the MMP and CDC light traps, and at least three times more P. papatasi than the light-only MCU and S360 traps (P < 0.05). Mean numbers (+/- SE) of P. papatasi captured per trap night were as follows: BGS 142.1 (+/- 45.8) > MMP 56.8 (+/- 9.0) > CDC 52.3 (+/- 6.1) > MCU 38.2 (+/- 6.4) > S360 12.6 (+/- 1.8). Results indicate that several types of commercial traps are suitable substitutes for the CDC light trap in sand fly surveillance programs.
Subject(s)
Insect Control/instrumentation , Psychodidae/physiology , Animals , Carbon Dioxide , Egypt , LightABSTRACT
Attractive toxic sugar baits (ATSBs) were used to control mosquitoes in the storm drains of a residential area on the outskirts of St Augustine, Florida. The drainage system was newly constructed and no mosquitoes were breeding inside it. The area covered by the storm drains was divided in half; 10 drains served as control drains and 16 drains served as experimental drains. The baits, which consisted of a mixture of brown sugar, fruit juice, green dye marker and boric acid, were presented at the entrances of the treated drains and exit traps were positioned over the drain openings and the connecting tubes leading to retention ponds. Similar baits with orange dye and without toxin were presented at the entrances of control drains. A total of 220 pupae of Culex quinquefasciatus (Diptera: Culicidae) were released in each control and toxin-treated drain, and the numbers of recovered mosquitoes were examined to determine the effectiveness of ATSBs in the storm drain system. An average of 178.2 mosquitoes exited each drain in the control area; 87.0% of these had fed on the baits and were stained orange, whereas 13.0% were unstained. In the toxin-treated drains, 83.7% of hatched females and 86.6% of hatched males were controlled by the baits.
Subject(s)
Boric Acids , Carbohydrates/chemistry , Culex , Insecticides , Mosquito Control/instrumentation , Mosquito Control/methods , Animals , Female , Florida , Male , Mosquito Control/standardsABSTRACT
A sampling study using a BG-Sentinel trap baited with CO(2), a gravid trap baited with an oak-pine infusion, a human subject, and a vegetative aspirator was conducted to compare their reliability at detecting Aedes albopictus Skuse in suburban and sylvatic habitats. We collected 73,849 mosquitoes, representing 29 species from 11 genera over a 20-wk period. The BG-Sentinel trap accounted for over 85% of all Ae. albopictus captured and was significantly more effective at detecting the presence of Ae. albopictus compared with the other three techniques. Landing counts provided the fewest mosquito species (n = 10), yet provided a quick and effective weekly assessment of the major biting species and were the most effective method for sampling Ae. albopictus within a 10-min period. Fewer Ae. albopictus were sampled from sylvatic habitats compared with suburban ones. Sampling criteria advantageous for surveying Ae. albopictus and other mosquito species are discussed.
Subject(s)
Aedes/drug effects , Insect Control/methods , Pheromones/pharmacology , Volatile Organic Compounds/pharmacology , Aedes/physiology , Animals , Culicidae/classification , Culicidae/physiology , Ecosystem , Female , Florida , Male , Random AllocationABSTRACT
To ascertain mosquito species-specific oviposition height preferences, a study was conducted evaluating the response of field populations of container-inhabiting mosquitoes to water, oak (Quercus spp.),or oak-pine (Pinus spp.) infusion-baited ovitraps in four suburban and four sylvatic habitats in north central Florida. In total, 48 ovitraps, 24 suspended at each height of 1 or 6 m (near the ground or tree canopy, respectively), were monitored weekly for 5 mo. Throughout our study, we collected 13,276 mosquito eggs, representing five species from four genera, the most common being Aedes triseriatus (Say), Aedes albopictus Skuse, and Orthopodomyia signifera (Coquillett). Significantly more Ae. triseriatus and Ae. albopictus eggs were oviposited in containers with oak and oak-pine infusions compared with water alone. Significantly more Ae. albopictus eggs were recovered from traps at 1 m in suburban habitats, whereas more Ae. triseriatus eggs were recovered at 6 m in sylvatic habitats.
Subject(s)
Culicidae/physiology , Environment , Oviposition , Animals , Behavior, Animal , Florida , Ovum/classification , Species SpecificityABSTRACT
The response of Aedes albopictus to the BG-Sentinel, Omni-directional-Fay-Prince, and Mosquito MagnetX traps was evaluated in four suburban and four sylvatic habitats in north-central Florida to ascertain potential height preference of this species. These traps, which are primarily designed to attract diurnal mosquitoes, were set at 1 and 6 m and were evaluated during 40 trapping periods over 4 mo. We collected 45,640 mosquitoes, representing 26 species from 10 genera, the most common being Aedes albopictus, Ae. vexans, Coquilletidia perturbans, Culex nigripalpus, Aedes infirmatus, Ae. triseriatus, and Psorophora ferox. Although significantly more Ae. albopictus were captured at 1 m above ground than at 6 m, fewer were captured in sylvatic habitats than suburban habitats. Although not statistically different, the BG-Sentinel caught more Ae. albopictus compared with the other two traps regardless of locale. These results suggest that, although Ae. albopictus was captured as high as 6 m, the majority seek hosts at or below 1-m heights. This further supports prior research that, although Ae. albopictus has been shown to disseminate West Nile virus, it has not been implicated as a major vector for the virus, which is likely because of its propensity to feed on ground-dwelling hosts. The study also shows how trap type, trap heights, and environments influence sampling estimates when determining species abundance.
Subject(s)
Aedes/physiology , Mosquito Control , Animals , Feeding Behavior , Female , Florida , MaleABSTRACT
We compared 6 adult mosquito traps for effectiveness in collecting Aedes albopictus from suburban backyards with the goal of finding a more suitable surveillance replacement for the Centers for Disease Control and Prevention (CDC) light trap. Trap selection included 2 commercial propane traps (Mosquito Magnet Professional trap and Mosquito Magnet Liberty trap), 2 Aedes-specific traps (Fay-Prince Omnidirectional trap and Wilton trap), 1 experimental trap (Mosquito Magnet-X trap), and a standard surveillance CDC light trap that served as a control. Traps that did not generate carbon dioxide were provided with bottled CO2 at a flow rate of 500 ml/min. Those traps designed for use with chemical attractants (Mosquito Magnet traps) were baited with Lurex (L-lactic acid) and octenol (1-octen-3-ol) commercial baits, known attractants to Ae. albopictus. Three repetitions of a 6 x 6 Latin square test yielded a total of 37,237 mosquitoes, of which 5,280 (14.2%) were Ae. albopictus. Significantly more (P < 0.05) Ae. albopictus were collected from the experimental and commercial traps (4,244/5,280; 80.3%) than from the CDC light trap and Aedes-specific traps. The Mosquito Magnet Liberty collected the most Ae. albopictus (1,591), accounting for 30.1% of the total take, followed closely by the Mosquito Magnet-X (1,468) and the Mosquito Magnet Pro (1,185). The omnidirectional Fay-Prince trap performed better than the CDC or Wilton trap. Twenty-seven mosquito species were collected during these trials, 9 species in large enough numbers for meaningful analysis. Aedes albopictus was the second most common mosquito trapped. The results of these trials indicate that propane-powered commercial traps would serve as useful substitutes in lieu of CDC traps in Ae. albopictus surveillance efforts. Trap features advantageous for collecting Ae. albopictus and other mosquito species are discussed.
Subject(s)
Aedes , Mosquito Control/instrumentation , Animals , Cues , Female , Florida , Male , Mosquito Control/methods , Sex RatioABSTRACT
Two types of commercial propane-powered traps, mosquito magnet (MM) (American Biophysics Corporation) MM-Freedom (Freedom) and MM-Liberty Plus (Liberty Plus), were evaluated for the collection of Culicoides. Trap preference and seasonal characteristics for the 3 major species, Culicoides furens, Culicoides barbosai, and Culicoides mississippiensis, were recorded from July 7, 2005, to July 24, 2006. Over 35 million Culicoides were captured during our study. When species were evaluated separately, analysis of overall mean trap collections yielded 5 months (February, March, June, September, and October) with significant trap effects. The Freedom trap captured more C. furens in June and October; the Liberty Plus trap captured more C. mississippiensis in February, March, and April, and more C. barbosai in September. The high numbers of Culicoides captured during our study suggest that the number of host-seeking Culicoides could potentially be reduced by continuous trapping during times when they are prevalent. Results of these investigations will be used to guide future control efforts.
Subject(s)
Ceratopogonidae , Mosquito Control/instrumentation , Seasons , Animals , Florida , Population DensityABSTRACT
Field studies were conducted at wooded wetlands in Gainesville, FL, U.S.A., to assess responses of natural populations of adult mosquitoes (Diptera: Culicidae) to American Biophysics MM-X and Coleman MD-2500 traps baited with enantiomers of 1-octen-3-ol, a naturally occurring compound, and 1-octyn-3-ol, a closely related synthetic compound. Overall, the same species of mosquitoes were attracted by all enantiomers, although the (R)-(+) isomer of octenol generally attracted more species, and it is the isomer produced in greatest proportion in nature. Traps baited with the R-enantiomer caught greater numbers of mosquitoes than those baited with the S-enantiomer of each compound, whereas traps baited with S-enantiomers were equally or slightly less attractive than those baited with carbon dioxide only.
Subject(s)
Alkynes/pharmacology , Culicidae/drug effects , Mosquito Control/methods , Octanols/pharmacology , Pheromones/pharmacology , Alkynes/chemistry , Animals , Culicidae/classification , Florida , Fresh Water , Isomerism , Octanols/chemistry , Pheromones/chemistry , Species SpecificityABSTRACT
The impact of the attractants l-octen-3-ol (octenol) and L-lactic acid (LurexTM) on collection of Aedes albopictus in suburban backyards was assessed in Mosquito Magnet Pro traps. These carbon dioxide-producing traps were additionally baited with commercial formulated lures with octenol, lactic acid, octenol + lactic acid, or no attractant (control) and evaluated in 4 residential sites. Three repetitions of the study resulted in the total collection of 1,321 Ae. albopictus. Significantly more Ae. albopictus were captured in traps baited with octenol + lactic acid than in traps baited only with octenol. Lactic acid-baited and control trap captures were not significantly different from octenol + lactic acid- or octenol-baited trap totals. Octenol- + lactic acid-baited traps collected 36.2% and 52.0% more Ae. albopictus than lactic acid-baited and control traps, respectively. Male Ae. albopictus accounted for 26.7% of the total capture. Other mosquito species collected in sufficient numbers for analysis included Cx. nigripalpus, Ochlerotatus infirmatus, Psorophoraferox, and Cx. erraticus. Larger numbers of these species were collected in traps that were unbaited or baited with only octenol than in traps baited with lactic acid or octenol + lactic acid.
Subject(s)
Aedes/drug effects , Appetitive Behavior/drug effects , Carbon Dioxide/pharmacology , Lactic Acid/pharmacology , Mosquito Control/methods , Octanols/pharmacology , Animals , Culicidae/drug effects , Female , Florida , MaleABSTRACT
Responses of the mosquitoes Aedes aegypti, Culex quinquefasciatus, and Culex nigripalpus to volatiles and compounds associated with bovine and avian blood that were presented in collagen membranes were evaluated in olfactometer and landing assays. The presence of attractants produced by blood was supported by more attraction of all species to blood than water controls in the olfactometer. Females of Ae. aegypti and Cx. quinquefasciatus were more attracted to bovine blood than to avian blood, but there was no difference in Cx. nigripalpus responses. In landing assays, significantly more females of all species landed on casings with blood than on water controls. There was no difference in landing of Ae. aegypti on bovine or avian blood. However, significantly more females of Cx. quinquefasciatus and Cx. nigripalpus landed on avian blood compared to bovine blood. Blood presented in collagen casings was an effective method for evaluating in-flight attraction and landing in all three species. In the olfactometer, several individual compounds elicited attraction in all species, but none were as attractive as blood for all species. In landing assays, several organic acids and sulfides elicited landing, with Ae. aegypti responding to the greatest number of compounds. These assay methods are effective for evaluation of volatile compounds from blood, and although responses were obtained to several compounds, none were as effective as blood in the olfactometer and landing assays.
Subject(s)
Aedes/physiology , Blood , Culex/physiology , Feeding Behavior , Odorants , Animals , Carboxylic Acids/blood , Cattle , Chickens , Collagen , Fatty Acids/blood , Female , Sulfides/blood , VolatilizationABSTRACT
Attraction of Culex quinquefasciatus Say, Culex tarsalis Coquillett, Culex nigripalpus Theobald, and Aedes aegypti (L.) to avian and other host odors was investigated in a dual-port olfactometer. Although attraction to a human arm was high for Ae. aegypti (>80%) and low for all Culex spp. (<25%), all species responded similarly to a chicken (55.3-73.6%). Responses of Ae. aegypti, Cx. quinquefasciatus, and Cx. nigripalpus to feathers were low (<20%) but greater than to controls. There was no difference in attraction of Cx. tarsalis to feathers or controls. Responses to CO2 (5 ml/min) were low for all species (<15%) except Cx. tarsalis, which were moderate (24.5%). When feathers were combined with CO2, the resulting attraction was additive or lower than responses to feather and CO2 alone for all species except for Cx. tarsalis, which had responses that were three-fold greater than expected if responses were additive. The CO2-feather treatments were less attractive than a chicken for all species. When olfactometer assays were extended from 3 to 20 min, responses by Ae. aegypti significantly increased to a chicken and CO2 and attraction of Cx. quinquefasciatus significantly greater to chickens, CO2, and feathers. None of the volatile compounds previously identified from feathers or uropygial glands tested were attractive. Both feather-rubbed cotton balls and hexane extracts of feathers were attractive and as attractive as feathers; however, ether extracts were not attractive. Feathers clearly contribute to the attraction of host-seeking Culex spp., and future studies will focus on identification of the attractant compounds.
Subject(s)
Appetitive Behavior/drug effects , Culex/physiology , Odorants , Organic Chemicals/pharmacology , Pheromones/standards , Animals , Carbon Dioxide , Chickens , Culex/drug effects , Feathers/chemistry , Female , Humans , Organic Chemicals/chemistry , Smell/drug effects , Smell/physiology , Time Factors , VolatilizationABSTRACT
A method of marking adult Culex quinquefasciatus by feeding the larvae commercial hog chow dyed with methylene blue, Giemsa, and crystal violet was evaluated under laboratory conditions. Of 243 mosquitoes fed the dyed food, 230 had visible marks (94.6%). The dyed food increased the egg-adult development time from 11.4 to 12.1 d. After 9 d, 82.5% of adult mosquitoes dyed as larvae could be identified, and remained detectable for up to 15 d, their maximum laboratory life.
Subject(s)
Coloring Agents/administration & dosage , Culex/physiology , Staining and Labeling/veterinary , Animal Feed , Animals , Azure Stains/administration & dosage , Azure Stains/pharmacology , Coloring Agents/pharmacology , Culex/growth & development , Gentian Violet/administration & dosage , Gentian Violet/pharmacology , Larva/drug effects , Larva/growth & development , Methylene Blue/administration & dosage , Methylene Blue/pharmacology , Staining and Labeling/methods , Staining and Labeling/standards , Time FactorsABSTRACT
Examples of the commercial trap Mosquito Magnet Pro (MMP emitting attractant 1-octen-3-ol in carbon dioxide 500 mL/min generated from propane fuel), were run 24 h/day on the Isle of Skye, Scotland, during June-August 2001 and evaluated for catching Culicoides biting midges (Diptera: Ceratopogonidae). From 30 days trapping, the catch averaged 2626 +/- 1358 Culicoides females/trap/day (mean +/- SE, range 558 +/- 139 to 6088 +/- 3597, for five sets of six consecutive nights), predominantly the pest Culicoides impunctatus Goetghebuer (68% overall), plus C. vexans (Staeger) > C. delta Edwards > C. pulicaris (L.) > C. lupicaris Downs & Kettle > C. albicans (Winnertz) > other Culicoides spp. Attempts were made to enhance the odour baiting system by adding hexane-extracts (2.1 mg/day) of hair samples from large host animals, resulting in the following effects on Culicoides collections: sheep - 53 %, red deer - 26 %, calf + 20%, pony + 40%, water buffalo + 262%, with greatest increases for C. impunctatus and C. pulicaris. Serial concentrations of these animal extracts (10(-1) - 10(-3) x 2.2 g/mL) were assayed on parous female C. impunctatus response in a Y-tube olfactometer (air-flow 150 mL/min), and by electroantennogram (EAG) on Culicoides nubeculosus Meigen laboratory-reared parous females. Positive behavioural responses to host odours were dose-dependent: the water buffalo extract being most active (threshold 0.22 g/mL), similar to deer, whereas other host extracts were > or = 10-fold less active. Correspondingly, the EAG threshold was lowest for water buffalo, 10-fold greater for deer, calf and pony, but not detected for sheep. If the active component(s) of these host extracts can be identified and synthesized, they might be employed to improve the capture of Culicoides midges for local control by removal trapping.
Subject(s)
Ceratopogonidae/physiology , Insect Control/methods , Pheromones/physiology , Animals , Behavior, Animal , Buffaloes , Cattle , Deer , Electrophysiology , Female , Horses , Scotland , SheepABSTRACT
A removal trapping method that employed individual lure-baited insect suction traps for the reduction of adult biting midges (Culicoides spp.) was evaluated in a coastal residential neighborhood of northwestern Florida during 2000. Six backyards were used in the study. Lure-baited traps consisted of one ABC PRO insect suction trap (fitted with a 30 by 30-mesh polyester screen bag) baited with carbon dioxide (500 ml/min) and a 4:1:8 mixture of 1 octen-3-ol, 3-n-propylphenol, and 4-methylphenol (5.39 +/- 0.54 mg/h) placed in each of three backyards. Lure-baited traps ran continuously during the 4-mo study. Three backyards without lure-baited traps served as controls. Adult midge populations were monitored twice weekly for 24-h in each backyard using an additional ABC PRO trap baited with carbon dioxide (500 ml/min) only. Five species of biting midges were collected from all traps in each backyard (in descending order): Culicoides mississippiensis, C. barbosai, C. melleus, C. furens, and C. hollensis. Overall reduction was not consistent in lure-baited backyards and ranged from 2.3% to 70.6% on 16 of the 30 sample dates. But when biting midge populations seasonally declined to a weekly average of < 500 midges per lure-baited trap, reduction occurred more frequently. Midge reduction did not appear to be species specific.
Subject(s)
Ceratopogonidae , Insect Control/methods , Animals , Florida , Insect Bites and Stings , Pheromones/pharmacology , Population DynamicsABSTRACT
The efficacy of Centers for Disease Control, ABC PRO, and counterflow geometry (CFG) mosquito suction traps to sample populations of adult Culicoides was investigated in northwestern Florida. These traps were baited either with a 4:1:8 mixture of octenol, 3-n-propylphenol, and 4-methylphenol alone or in combination with carbon dioxide (CO2). Control traps were operated without the octenol-phenol mixture or CO2. Four species, in order of descending abundance, were collected in all traps regardless of treatment: Culicoides mississippiensis, C. barbosai, C melleus, and C. furens. Midge abundance from traps baited with octenolphenol alone was not significantly different, regardless of species, when compared with traps without the mixture. However, when CO2 or CO2 plus the mixture was used, trap collections of C. mississippiensis and C. barbosai significantly increased, with the latter mixture exhibiting a synergistic effect on trap catch for both species. When this combination was used, the ABC PRO trap collected significantly more C. mississippiensis, whereas the CFG trap caught significantly more C. barbosai compared with all traps powered with 6-V batteries (P < 0.05). The effects of CO2 plus the octenol-phenol mixture on C. melleus collections appeared to be additive only for ABC PRO and CFG traps. Populations of C. furens were sporadic and too low (<0.5% of total collection) to determine any statistically meaningful differences. On the average, CFG traps powered with 12-V batteries only increased midge collection 1.2 times compared with similar traps powered by 6-V batteries. This increase was not significantly different (P > 0.05).
Subject(s)
Ceratopogonidae , Insect Control/methods , Animals , Carbon Dioxide , Cresols , Florida , Octanols , PhenolsABSTRACT
Volatile compounds emanated from human skin were studied by gas chromatography/mass spectrometry (GC/MS). The purpose of this study was to identify compounds that may be human-produced kairomones which are used for host location by the mosquito, Aedes aegypti (L.). The procedure used to collect volatiles was chosen because of prior knowledge that attractive substances can be transferred from skin to glass by handling. Laboratory bioassays have shown that the residuum on the glass remains attractive to mosquitoes until the compounds of importance evaporate. The sampling and analytical procedures modeled the above-cited process as closely as possible except that the evaporation of compounds from the glass surface was accomplished by thermal desorption from glass beads in a heated GC injection port. This made possible the solventless injection of volatiles onto the column. The compounds were cryofocused on the head of the column with liquid nitrogen prior to GC separation. A single stage of mass spectrometry on a triple quadrupole instrument was used for mass analysis. A combination of electron ionization and pulsed positive ion/negative ion chemical ionization modes on two different GC columns (one polar, one relatively nonpolar) was used to identify most of the 346 compound peaks detected by this technique.