Effect of phosphorus precursors on the structure of bioactive calcium phosphate silicate systems.

The main aim of this work was to synthesize calcium phosphate silicate bioceramics by a low energy-consuming sol-gel method applying various phosphorous precursors (triethyl phosphate, phosphoric acid, and ammonium hydrogen phosphate). The investigations concentrated on the influence of phosphorous initial compounds on the bond and crystalline structures and the material quality. The application of the alkoxide and inorganic P-precursors results in considerably different textures. The inorganic PO4-containing precursors lead to sol formations. The sol systems can be characterized by a randomly bonded aggregate structure. Monolith gel systems can only be prepared by using TEP. The alkoxide P-precursor more effectively furthers the connection between the phosphorous and silicon tetrahedra than the inorganic phosphate compounds. Over the P-precursors, the catalyst also affects the structure and properties. In the present work, a special attention was paid to identify the POSi bonds in the FTIR and 31P NMR spectra. The bond systems were investigated by FTIR, 31P and 29Si MAS NMR spectroscopies, the morphology by SEM, WAXS, and XRD measurements, and the water solubility of the ceramic systems also was tested.

Molecular interaction of a new antibacterial polymer with a supported lipid bilayer measured by an in situ label-free optical technique.

The interaction of the antibacterial polymer-branched poly(ethylene imine) substituted with quaternary ammonium groups, PEO and alkyl chains, PEI25QI5J5A815-with a solid supported lipid bilayer was investigated using surface sensitive optical waveguide spectroscopy. The analysis of the optogeometrical parameters was extended developing a new composite layer model in which the structural and optical anisotropy of the molecular layers was taken into consideration. Following in situ the change of optical birefringence we were able to determine the composition of the lipid/polymer surface layer as well as the displacement of lipid bilayer by the antibacterial polymer without using additional labeling. Comparative assessment of the data of layer thickness and optical anisotropy helps to reveal the molecular mechanism of antibacterial effect of the polymer investigated.