ABSTRACT
Revealing of enterochromaffine and enterochromaffine-like cells of the rat stomach was undertaken under the effect of bombesin and alpha- and beta-adrenoblocking agents. Fibers containing gastrin-releasing-peptide were immunohistochemically revealed in neural plexus of the rat digestive tract. Spontaneous rhythmic release of the stomach monoamine cells' secret was shown. The effect of bombesin on the enterochromaffine-like cells of the rat stomach fundus containing histamine, was shown to be actualized through beta-adrenoreceptors. Serotonin excretion from the enterochromaffine cells of the stomach antrum depends neither on bombesin nor on the adrenergic effects.
Subject(s)
Biogenic Amines/metabolism , Bombesin/pharmacology , Chromaffin System/metabolism , Enterochromaffin Cells/metabolism , Gastric Mucosa/metabolism , Animals , Fluorescent Antibody Technique , Gastric Acid/metabolism , Gastrin-Releasing Peptide , Histamine/metabolism , Immunoenzyme Techniques , Peptides/pharmacology , Rats , Serotonin/metabolismABSTRACT
Using antisera against serotonin (5-OT) and neurotensin (NT) with immunofluorescence, 5-OT-immunoreactive and NT-immunoreactive nerve fibers have been detected in the walls of the femoral and pial arteries and intragastric arterioles. With electron microscopy, adrenergic and cholinergic axons were found in close anatomical proximity to some intragastric capillaries and arterioles. A possible role of the detected nerves is being discussed.
Subject(s)
Femoral Artery/innervation , Pia Mater/blood supply , Stomach/blood supply , Animals , Arterioles/innervation , Arterioles/ultrastructure , Femoral Artery/ultrastructure , Fluorescent Antibody Technique , Histocytochemistry , Microscopy, Electron , Pia Mater/ultrastructure , Rats , Rats, Inbred Strains , Stomach/innervation , Stomach/ultrastructureABSTRACT
Localization of serotonin (5-OT)- and neurotensin (NT)-containing cells in the tunica mucosa of the gastric antral part and the duodenum of the rat and chick has been revealed by means of the immunofluorescent method and the method with application of the peroxidase-antiperoxidase complex. For immune-histochemical revealing of biologically active substances in endocrine cells Sovient antisera are used for the first time. Experiments are performed on injection of antiserotonin serum of various dosage into the rat blood bed. The reaction of serotonin-containing cells of the gastric antral part tunical mucosa and those of the duodenum to changes in serotonin concentration in blood serum is demonstrated.