Phytochemical study on the essential oils of Callitris glaucophylla Joy Thomps. & L.A.S. Johnson, and assessment of their antioxidant, anti-enzymatic and allelopathic effects.

Callitris glaucophylla Joy Thomps. & L.A.S. Johnson is a coniferous forest species of the Cupressaceae family native to Australia. This species is rich in essential oils (EOs) but few studies about variability and biological activity of these EOs are available in the literature. The purpose of this study was to evaluate the variability of production of C. glaucophylla EOs in relation to the different plant parts (needles, cones and stems) and to investigate their antioxidant, anti-enzymatic and herbicidal properties. EOs were obtained by hydro distillation and analyzed by GC and GC-MS. The antioxidant potential of EOs was assessed by ABTS, FRAP and DPPH assays, their phytotoxic activities were evaluated against germination and shoots and radical growth of Sinapis arvensis, Trifolium campestre, Lepidium sativum and Lolium rigidum. The EOs were evaluated for their possible anti-enzymatic effects with spectrophotometric assay. EOs resulted rich in monoterpenes hydrocarbons (61.04-77.82 %) and oxygenated monoterpenes (19.52-25.26 %). The main compounds were α-pinene as major compound in all plant parts (36.99-59.84 %), 1,8-cineole (19.88 % in stems) and limonene (18.94 % in needles). Herbicidal assays showed that all EOs have remarkable and significant phytotoxicity towards germination, roots, and aerial parts growth of the tested plants, depending on the EO, the doses and tested species. The EOs showed significant free radical scavenging potential and resulted more active against cholinesterases than α-glucosidase and α-amylase. The data obtained constitute an important contribution in selecting and valorizing appropriate forestry tree biomass as sources of antioxidant and phytotoxic molecules for sustainable application in food preservation and weeds control. The activities against the tested enzymes confirmed a possible use of these EOs as natural pesticides.

Investigation on Chemical Composition, Antioxidant, Antifungal and Herbicidal Activities of Volatile Constituents from Deverra tortuosa (Desf.).

This study aims to analyze the chemical composition of the essential oils (EOs) obtained from stems and umbels of D. tortuosa as well the assessment of their biological activity. EOs were extracted by hydrodistillation and analyzed by gas chromatography coupled to mass spectrometry (GC/MS). The antioxidant properties were determined by DPPH and ABTS assays. The phytotoxic potential was assessed against dicots weeds (Sinapis arvensis and Trifolium campestre), monocots weeds (Lolium rigidum) and the crop Lepidium sativum. The antifungal activity was evaluated against four target phytopathogenic fungal strains. High diversity of compounds was detected in D. tortuosa Eos, varying among plant parts and consisting mainly of α-pinene (24.47-28.56%), sabinene (16.2-18.6%), α-phellandrene (6.3-11.7%) and cis-ocimene (5.28-7.85%). D. tortuosa EOs exhibited remarkable antioxidant activity, as well as interesting variable antifungal activities depending on the dose and fungi strain. The herbicidal activity of EOs showed significant efficacy on the inhibition of germination and seedling growth of all tested herbs. These results suggest that the EOs of Deverra tortuosa represent a valuable source of antioxidant, antifungal and phytotoxic metabolites and could be potential candidates for pest management, contributing to the promotion of sustainable agriculture.

Essential Oils and Biological Activities of Eucalyptus falcata, E. sideroxylon and E. citriodora Growing in Tunisia.

Many plants are able to synthesize essential oils (EOs), which play key roles in defense against weeds, fungi and pests. This study aims to analyze the chemical composition and to highlight the antioxidant, antimicrobial and phytotoxic properties of the EOs from Eucalyptus falcata, E. sideroxylon and E. citriodora growing in Tunisia. EOs were analyzed by gas chromatography coupled to mass spectrometry (GC/MS) and their antioxidant properties were determined by total antioxidant capacity (TAC), DPPH and ABTS assays. The phytotoxic potential was assessed against weeds (Sinapis arvensis, Phalaris canariensis) and durum wheat crop (Triticum durum) and compared to chemical herbicide glyphosate. The antifungal activity was investigated in vitro against eight target fungal strains. All EOs displayed a specific richness in oxygenated monoterpenes (51.3-90%) and oxygenated sesquiterpenes (4.8-29.4%), and 1,8-cineole, citronellal, citronellol, trans-pinocarveol, globulol, spathulenol and citronellyl acetate were the main constituents. Eucalyptus EOs exhibited remarkable antioxidant activity and E. citriodora oil exhibited significant activity when compared with E. falcata and E. sideroxylon EOs. The phytotoxic potential of the tested oils had different efficacy on seed germination and the growth of seedlings and varied among tested herbs and their chemical composition variability. Their effectiveness was better than that of glyphosate. At the post-emergence stage, symptoms of chlorosis and necrosis were observed. Furthermore, a decrease in chlorophyll and relative water content, electrolyte leakage and high levels of MDA and proline were indicators of the oxidative effects of EOs and their effectiveness as bioherbicides. Moreover, all the EOs exhibited moderate fungitoxic properties against all the tested fungal strains. Therefore, according to the obtained results, Eucalyptus EOs could have potential application as natural pesticides.