The Spectrophotometric Characteristic of Immunoglobulin Conjugates for Diagnostics of Causative Agents of Especially Dangerous Infections.

The possibility of characterizing fluorescent immunoglobulins using spectrophotometric analysis as a testing method is considered. The comparative analysis of optical properties of fluorescent immunoglobulin preparations and their components-immunoglobulins and fluorochrome-is carried out. The obtained results testify that the proposed methodological approach of optical detection of labeled immunoglobulin molecules can be promising for tests on obtaining conjugates used in immunological tests on revealing specific antigens of causative agents of especially dangerous infections.

[Determination of glucose diffusion coefficient in the human eye sclera]. / Opredelenie koéffitsienta diffuzii gliukozy v sklere glaza cheloveka.

The diffusion coefficients of aqueous glucose solutions in human sclera in vitro were estimated. The method is based on measurements of time-dependence of collimated transmittance of scleral samples under the action of biocompatible immersion liquids. It was shown that changes in collimated transmittance are connected with the matching between the indices of refraction of scleral scatterers and interstitial fluid. The dynamics of interstitial fluid replacement was registered by recording successively the collimated transmittance spectra in the range of 400-800 nm. For a quantitative description of the replacement process, a diffusion model was proposed, which assumes that the diffusion coefficient is constant throughout the volume of the scleral sample. Experimental results for glucose solutions of concentrations 0.18, 0.3, and 0.4 g/ml are presented. The diffusion coefficients were estimated by approximating the experimental data in the framework of the proposed model.