ABSTRACT
Mutations in the Cyclin-dependent kinase-like 5 (CDKL5) gene cause severe neurodevelopmental disorders accompanied by intractable epilepsies, i.e. West syndrome or atypical Rett syndrome. Here we report generation of the Cdkl5 knockout mouse and show that CDKL5 controls postsynaptic localization of GluN2B-containing N-methyl-d-aspartate (NMDA) receptors in the hippocampus and regulates seizure susceptibility. Cdkl5 -/Y mice showed normal sensitivity to kainic acid; however, they displayed significant hyperexcitability to NMDA. In concordance with this result, electrophysiological analysis in the hippocampal CA1 region disclosed an increased ratio of NMDA/α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor-mediated excitatory postsynaptic currents (EPSCs) and a significantly larger decay time constant of NMDA receptor-mediated EPSCs (NMDA-EPSCs) as well as a stronger inhibition of the NMDA-EPSCs by the GluN2B-selective antagonist ifenprodil in Cdkl5 -/Y mice. Subcellular fractionation of the hippocampus from Cdkl5 -/Y mice revealed a significant increase of GluN2B and SAP102 in the PSD (postsynaptic density)-1T fraction, without changes in the S1 (post-nuclear) fraction or mRNA transcripts, indicating an intracellular distribution shift of these proteins to the PSD. Immunoelectron microscopic analysis of the hippocampal CA1 region further confirmed postsynaptic overaccumulation of GluN2B and SAP102 in Cdkl5 -/Y mice. Furthermore, ifenprodil abrogated the NMDA-induced hyperexcitability in Cdkl5 -/Y mice, suggesting that upregulation of GluN2B accounts for the enhanced seizure susceptibility. These data indicate that CDKL5 plays an important role in controlling postsynaptic localization of the GluN2B-SAP102 complex in the hippocampus and thereby regulates seizure susceptibility, and that aberrant NMDA receptor-mediated synaptic transmission underlies the pathological mechanisms of the CDKL5 loss-of-function.
Subject(s)
Hippocampus/metabolism , Post-Synaptic Density/metabolism , Protein Serine-Threonine Kinases/metabolism , Receptors, N-Methyl-D-Aspartate/metabolism , Seizures/metabolism , Animals , Disease Models, Animal , Disease Susceptibility/metabolism , Excitatory Amino Acid Antagonists/pharmacology , Guanylate Kinases/metabolism , Hippocampus/drug effects , Hippocampus/pathology , Kainic Acid , Membrane Proteins/metabolism , Mice, Inbred C57BL , Mice, Knockout , N-Methylaspartate , Piperidines/pharmacology , Post-Synaptic Density/drug effects , Post-Synaptic Density/pathology , Protein Serine-Threonine Kinases/genetics , RNA, Messenger/metabolism , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors , Seizures/pathology , Tissue Culture TechniquesABSTRACT
Elimination of granule cells (GCs) in the olfactory bulb (OB) is not a continuous event but is rather promoted during short time windows associated with the animal's behavior. We previously showed that apoptotic GC elimination is enhanced during food eating and subsequent rest or sleep, and that top-down inputs from the olfactory cortex (OC) to the OB during the postprandial period are the crucial signal promoting GC elimination. However, whether enhanced GC elimination occurs during behaviors other than postprandial behavior is not clear. Here, we investigated whether exposure to noxious stimulation promotes apoptotic GC elimination in mice. Mice were delivered a brief electrical foot shock, during and immediately after which they showed startle and fear responses. Surprisingly, the number of apoptotic GCs increased 2-fold within 10 min after the start of foot shock delivery. This enhancement of GC apoptosis was significantly suppressed by injection of the GABAA receptor agonist muscimol in the OC, despite these muscimol-injected mice showing similar behavioral responses by foot shock as control mice. These results indicate that GC elimination is promoted in foot shock-delivered mice within a short time period of startle and fear responses. They also indicate that OC activity plays a central role in the enhanced GC elimination during this period, as is also the case in GC elimination during the postprandial period.
Subject(s)
Interneurons/pathology , Olfactory Bulb/pathology , Stress, Psychological/pathology , Animals , Apoptosis , Cell Count , Electric Stimulation , Fear/drug effects , GABA-A Receptor Agonists/pharmacology , Interneurons/drug effects , Male , Mice, Inbred C57BL , Muscimol/pharmacology , Olfactory Bulb/drug effects , Reflex, Startle/drug effects , Stress, Psychological/psychologyABSTRACT
Elimination of granule cells (GCs) in the olfactory bulb (OB) is not a continual event but is promoted during a short time window in the postprandial period, typically with postprandial sleep. However, the neuronal mechanisms for the enhanced GC elimination during the postprandial period are not understood. Here, we addressed the question of whether top-down inputs of centrifugal axons from the olfactory cortex (OC) during the postprandial period are involved in the enhanced GC elimination in the OB. Electrical stimulation of centrifugal axons from the OC of anesthetized mice increased GC apoptosis. Furthermore, pharmacological suppression of top-down inputs from the OC to the OB during the postprandial period of freely behaving mice by γ-aminobutyric acid (GABA)A receptor agonist injection in the OC significantly decreased GC apoptosis. Remarkable apoptotic GC elimination in the sensory-deprived OB was also suppressed by pharmacological blockade of top-down inputs. These results indicate that top-down inputs from the OC to the OB during the postprandial period are the crucial signal promoting GC elimination, and suggest that the life and death decision of GCs in the OB is determined by the interplay between bottom-up sensory inputs from the external world and top-down inputs from the OC.