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1.
Am J Physiol Heart Circ Physiol ; 323(6): H1091-H1107, 2022 12 01.
Article in English | MEDLINE | ID: mdl-36269647

ABSTRACT

Many anticancer therapies cause serious cardiovascular complications that degrade quality of life and cause early mortality in treated patients. Specifically, doxorubicin is known as an effective anticancer agent that causes cardiomyopathy in treated patients. There has been growing interest in defining the role of endothelial cells in cardiac damage by doxorubicin. We have shown in the present study that endothelial nuclei accumulate more intravenously administered doxorubicin than other cardiac cell types. Doxorubicin enhanced cardiac production of the transforming growth factor-ß (TGF-ß) ligands and nuclear translocation of phospho-Smad3 in both cultured and in vivo cardiac endothelial cells. To examine the role of the TGF-ß/mothers against decapentaplegic homolog 3 (Smad3) pathway in cardiac damage by doxorubicin, we used both Smad3 shRNA stable endothelial cell lines and Smad3-knockout mice. We demonstrated using endothelial transcriptome analysis that upregulation of the TGF-ß and inflammatory cytokine/cytokine receptor pathways, as well as suppression of cell cycle and angiogenesis by doxorubicin, were alleviated in Smad3-deficient endothelial cells. The results of transcriptomic analysis were validated using qPCR, immunoblotting, and ex vivo aortic ring sprouting assays. Similarly, increased cardiac expression of cytokines and chemokines observed in treated wild-type mice was diminished in treated Smad3-knockout animals. We also detected increased end-diastolic diameter and depressed systolic function in doxorubicin-treated wild-type but not Smad3-knockout mice. This work provides evidence for the critical role of the canonical TGF-ß/Smad3 pathway in cardiac damage by doxorubicin.NEW & NOTEWORTHY Microvascular endothelial cells in the heart accumulate more intravenously administered doxorubicin than nonendothelial cardiac cell types. The treatment enhanced the TGF-ß/Smad3 pathway and elicited endothelial cell senescence and inflammatory responses followed by adverse cardiac remodeling and dysfunction in wild-type but not Smad3-deficient animals. Our study suggests that the TGF-ß/Smad3 pathway contributes to the development of doxorubicin cardiomyopathy and the potential value of novel approaches to ameliorate cardiotoxicity by targeting the Smad3 transcription factor.


Subject(s)
Cardiomyopathies , Endothelial Cells , Mice , Animals , Endothelial Cells/metabolism , Quality of Life , Smad3 Protein/genetics , Smad3 Protein/metabolism , Doxorubicin/toxicity , Transforming Growth Factor beta/metabolism , Mice, Knockout
2.
J Mol Cell Cardiol ; 90: 129-38, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26686989

ABSTRACT

Elevated ALK4/5 ligands including TGF-ß and activins have been linked to cardiovascular remodeling and heart failure. Doxorubicin (Dox) is commonly used as a model of cardiomyopathy, a condition that often precedes cardiovascular remodeling and heart failure. In 7-8-week-old C57Bl/6 male mice treated with Dox we found decreased capillary density, increased levels of ALK4/5 ligand and Smad2/3 transcripts, and increased expression of Smad2/3 transcriptional targets. Human cardiac microvascular endothelial cells (HCMVEC) treated with Dox also showed increased levels of ALK4/5 ligands, Smad2/3 transcriptional targets, a decrease in proliferation and suppression of vascular network formation in a HCMVEC and human cardiac fibroblasts co-culture assay. Our hypothesis is that the deleterious effects of Dox on endothelial cells are mediated in part by the activation of the TGF-ß pathway. We used the inhibitor of ALK4/5 kinases SB431542 (SB) in concert with Dox to ascertain the role of TGF-ß pathway activation in doxorubicin induced endothelial cell defects. SB prevented the suppression of HCMVEC proliferation in the presence of TGF-ß2 and activin A, and alleviated the inhibition of HCMVEC proliferation by Dox. SB also prevented the suppression of vascular network formation in co-cultures of HCMVEC and human cardiac fibroblasts treated with Dox. Our results show that the inhibition of the TGF-ß pathway alleviates the detrimental effects of Dox on endothelial cells in vitro.


Subject(s)
Doxorubicin/pharmacology , Endothelial Cells/drug effects , Fibroblasts/drug effects , Transforming Growth Factor beta2/pharmacology , Activin Receptors, Type I/genetics , Activin Receptors, Type I/metabolism , Activins/genetics , Activins/metabolism , Activins/pharmacology , Animals , Benzamides/pharmacology , Cell Line , Coculture Techniques , Dioxoles/pharmacology , Doxorubicin/antagonists & inhibitors , Endothelial Cells/cytology , Endothelial Cells/metabolism , Fibroblasts/cytology , Fibroblasts/metabolism , Gene Expression Regulation , Human Umbilical Vein Endothelial Cells , Humans , Male , Mice , Mice, Inbred C57BL , Myocardium/cytology , Myocardium/metabolism , Protein Serine-Threonine Kinases/genetics , Protein Serine-Threonine Kinases/metabolism , Receptor, Transforming Growth Factor-beta Type I , Receptors, Transforming Growth Factor beta/genetics , Receptors, Transforming Growth Factor beta/metabolism , Signal Transduction , Smad2 Protein/genetics , Smad2 Protein/metabolism , Smad3 Protein/genetics , Smad3 Protein/metabolism , Transforming Growth Factor beta2/antagonists & inhibitors , Transforming Growth Factor beta2/genetics , Transforming Growth Factor beta2/metabolism
3.
J Proteomics ; 74(5): 683-97, 2011 May 01.
Article in English | MEDLINE | ID: mdl-21338723

ABSTRACT

Doxorubicin-induced cardiomyopathy in cancer patients is well established. The proposed mechanism of cardiac damage includes generation of reactive oxygen species, mitochondrial dysfunction and cardiomyocyte apoptosis. Exposure of adult rat cardiomyocytes to low levels of DOX for 48h induced apoptosis. Analysis of protein expression showed a differential regulation of several key proteins including the voltage dependent anion selective channel protein 2 and methylmalonate semialdehyde dehydrogenase. In comparison, proteomic evaluation of DOX-treated rat heart showed a slightly different set of protein changes that suggests nuclear accumulation of DOX. Using a new solubilization technique, changes in low abundant protein profiles were monitored. Altered protein expression, modification and function related to oxidative stress response may play an important role in DOX cardiotoxicity.


Subject(s)
Antibiotics, Antineoplastic/adverse effects , Cardiomyopathies/metabolism , Doxorubicin/adverse effects , Gene Expression Regulation/drug effects , Muscle Proteins/biosynthesis , Myocytes, Cardiac/metabolism , Animals , Antibiotics, Antineoplastic/pharmacology , Apoptosis/drug effects , Cardiomyopathies/chemically induced , Cardiotoxins/adverse effects , Cardiotoxins/pharmacology , Doxorubicin/pharmacology , Male , Myocytes, Cardiac/pathology , Oxidative Stress/drug effects , Protein Biosynthesis/drug effects , Protein Processing, Post-Translational/drug effects , Rats , Rats, Sprague-Dawley , Reactive Oxygen Species/metabolism , Time Factors
4.
Biophys J ; 96(4): 1388-98, 2009 Feb 18.
Article in English | MEDLINE | ID: mdl-19217856

ABSTRACT

Doxorubicin (DOX) is used for treating various cancers. Its clinical use is, however, limited by its dose-limiting cardiomyopathy. The exact mechanism of DOX-induced cardiomyopathy still remains unknown. The goals were to investigate the molecular mechanism of DOX-induced cardiomyopathy and cardioprotection by mitoquinone (Mito-Q), a triphenylphosphonium-conjugated analog of coenzyme Q, using a rat model. Rats were treated with DOX, Mito-Q, and DOX plus Mito-Q for 12 weeks. The left ventricular function as measured by two-dimensional echocardiography decreased in DOX-treated rats but was preserved during Mito-Q plus DOX treatment. Using low-temperature ex vivo electron paramagnetic resonance (EPR), a time-dependent decrease in heme signal was detected in heart tissues isolated from rats administered with a cumulative dose of DOX. DOX attenuated the EPR signals characteristic of the exchange interaction between cytochrome c oxidase (CcO)-Fe(III) heme a3 and CuB. DOX and Mito-Q together restored these EPR signals and the CcO activity in heart tissues. DOX strongly downregulated the stable expression of the CcO subunits II and Va and had a slight inhibitory effect on CcO subunit I gene expression. Mito-Q restored CcO subunit II and Va expressions in DOX-treated rats. These results suggest a novel cardioprotection mechanism by Mito-Q during DOX-induced cardiomyopathy involving CcO.


Subject(s)
Cardiomyopathies/drug therapy , Cardiotonic Agents/pharmacology , Doxorubicin/pharmacology , Electron Transport Complex IV/metabolism , Myocardium/enzymology , Organophosphorus Compounds/pharmacology , Ubiquinone/pharmacology , Animals , Apoptosis/drug effects , Body Weight/drug effects , Cardiomyopathies/chemically induced , Cardiomyopathies/physiopathology , Cardiotonic Agents/therapeutic use , Doxorubicin/toxicity , Electron Spin Resonance Spectroscopy , Endomyocardial Fibrosis/drug therapy , Heart/drug effects , Heart/physiology , Heme/physiology , Male , Mitochondria, Heart/drug effects , Mitochondria, Heart/enzymology , Organophosphorus Compounds/therapeutic use , Random Allocation , Rats , Rats, Sprague-Dawley , Ubiquinone/therapeutic use
5.
Free Radic Biol Med ; 45(12): 1723-8, 2008 Dec 15.
Article in English | MEDLINE | ID: mdl-18926904

ABSTRACT

A proposed mechanism for the cardiotoxicity of doxorubicin (DOX) involves apoptosis in cardiomyocytes. In the study described here, we investigated the molecular basis for the differences in DOX-induced toxicity in adult rat cardiomyocytes (ARCM), neonatal rat cardiomyocytes (NRCM), and rat embryonic H9c2 cardiomyoblasts. Activation of caspase-9 and -3 was considerably lower in DOX-treated ARCM as compared with NRCM and H9c2 cardiomyoblasts. Addition of cytochrome c caused the activation of caspase-9 and -3 in permeabilized NRCM and H9c2 cardiomyoblasts but not in permeabilized ARCM. Expression of proapoptotic proteins, apoptotic protease activating factor-1 (Apaf1), and procaspase-9 was significantly lower, and abundance of antiapoptotic X-linked inhibitor of apoptosis protein (XIAP) was higher in ARCM, as compared with immature cardiac cells. Despite the abundance of XIAP in ARCM, its role in the inhibition of apoptosome function was dismissed, as second mitochondria-derived activator of caspases (Smac)-N7 peptide, had no effect on caspase activation in response to cytochrome c in these cells. Adenoviral expression of Apaf1 exacerbated the activation of caspase-9 and -3 in DOX-treated NRCM, but did not increase their activities in DOX-treated ARCM. This finding points to a major difference in the apoptotic signaling between immature and adult cardiomyocytes. The mitochondrial apoptotic pathway is limited in ARCM treated with DOX.


Subject(s)
Antibiotics, Antineoplastic/pharmacology , Apoptosis/drug effects , Apoptosomes/drug effects , Doxorubicin/pharmacology , Myocytes, Cardiac/drug effects , Signal Transduction/drug effects , Adenoviridae/genetics , Animals , Animals, Newborn , Apoptosis Regulatory Proteins , Apoptosomes/metabolism , Apoptotic Protease-Activating Factor 1/genetics , Apoptotic Protease-Activating Factor 1/metabolism , Carrier Proteins/metabolism , Caspases/metabolism , Cytochromes c/metabolism , Enzyme Activation/drug effects , Immunoblotting , Male , Mitochondrial Proteins/metabolism , Myoblasts, Cardiac/cytology , Myoblasts, Cardiac/drug effects , Myoblasts, Cardiac/metabolism , Myocytes, Cardiac/cytology , Myocytes, Cardiac/metabolism , Rats , Rats, Sprague-Dawley , Subcellular Fractions , X-Linked Inhibitor of Apoptosis Protein/genetics , X-Linked Inhibitor of Apoptosis Protein/metabolism
6.
Biochem J ; 389(Pt 2): 527-39, 2005 Jul 15.
Article in English | MEDLINE | ID: mdl-15799720

ABSTRACT

Doxorubicin (DOX), a widely used antitumour drug, causes dose-dependent cardiotoxicity. Cardiac mitochondria represent a critical target organelle of toxicity during DOX chemotherapy. Proposed mechanisms include generation of ROS (reactive oxygen species) and disturbances in mitochondrial calcium homoeostasis. In the present study, we probed the mechanistic link between mitochondrial ROS and calcium in the embryonic rat heart-derived H9c2 cell line and in adult rat cardiomyocytes. The results show that DOX stimulates calcium/calcineurin-dependent activation of the transcription factor NFAT (nuclear factor of activated T-lymphocytes). Pre-treatment of cells with an intracellular calcium chelator abrogated DOX-induced nuclear NFAT translocation, Fas L (Fas ligand) expression and caspase activation, as did pre-treatment of cells with a mitochondria-targeted antioxidant, Mito-Q (a mitochondria-targeted antioxidant consisting of a mixture of mitoquinol and mitoquinone), or with adenoviral-over-expressed antioxidant enzymes. Treatment with GPx-1 (glutathione peroxidase 1), MnSOD (manganese superoxide dismutase) or a peptide inhibitor of NFAT also inhibited DOX-induced nuclear NFAT translocation. Pre-treatment of cells with a Fas L neutralizing antibody abrogated DOX-induced caspase-8- and -3-like activities during the initial stages of apoptosis. We conclude that mitochondria-derived ROS and calcium play a key role in stimulating DOX-induced 'intrinsic and extrinsic forms' of apoptosis in cardiac cells with Fas L expression via the NFAT signalling mechanism. Implications of ROS- and calcium-dependent NFAT signalling in DOX-induced apoptosis are discussed.


Subject(s)
Calcium/metabolism , Doxorubicin/pharmacology , Membrane Glycoproteins/genetics , Mitochondria, Heart/drug effects , NFATC Transcription Factors/metabolism , Nerve Tissue Proteins/metabolism , Reactive Oxygen Species/metabolism , Transcription, Genetic/drug effects , Tumor Necrosis Factors/genetics , Animals , Apoptosis/drug effects , Calcium/antagonists & inhibitors , Caspases/metabolism , Cell Line , Cell Nucleus/drug effects , Cell Nucleus/metabolism , Enzyme Activation/drug effects , Fas Ligand Protein , Gene Expression Regulation/drug effects , Hydrogen Peroxide/pharmacology , Male , Mitochondria, Heart/metabolism , Myocytes, Cardiac/cytology , Myocytes, Cardiac/metabolism , NFATC Transcription Factors/antagonists & inhibitors , Nerve Tissue Proteins/antagonists & inhibitors , Oxidative Stress , Rats , Rats, Sprague-Dawley , Signal Transduction/drug effects , Up-Regulation
7.
J Biol Chem ; 279(24): 25535-43, 2004 Jun 11.
Article in English | MEDLINE | ID: mdl-15054096

ABSTRACT

Doxorubicin (DOX), a widely used chemotherapeutic agent, exhibits cardiotoxicity as an adverse side effect in cancer patients. DOX-mediated cardiomyopathy is linked to its ability to induce apoptosis in endothelial cells and cardiomyocytes by activation of p53 protein and reactive oxygen species. We evaluated the potential roles of H(2)O(2) and p53 in DOX-induced apoptosis in normal bovine aortic endothelial cells and adult rat cardiomyocytes and in tumor cell lines PA-1 (human ovarian teratocarcinoma) and MCF-7 (human breast adenocarcinoma). Time course measurements indicated that activation of caspase-3 preceded the stimulation of p53 transcriptional activity in endothelial cells. In contrast, DOX caused early activation of p53 in tumor cells that was followed by caspase-3 activation and DNA fragmentation. These findings suggest that the transcriptional activation of p53 in DOX-induced apoptosis in endothelial cells may not be as crucial as it is in tumor cells. Further evidence was obtained using a p53 inhibitor, pifithrin-alpha. Pifithrin-alpha completely suppressed DOX-induced activation of p53 in both normal and tumor cell lines and prevented apoptosis in tumor cell lines but not in endothelial cells and cardiomyocytes. In contrast, detoxification of H(2)O(2), either by redox-active metalloporphyrin or overexpression of glutathione peroxidase, decreased DOX-induced apoptosis in endothelial cells and cardiomyocytes but not in tumor cells. This newly discovered mechanistic difference in DOX-induced apoptotic cell death in normal versus tumor cells will be useful in developing drugs that selectively mitigate the toxic side effects of DOX without affecting its antitumor action.


Subject(s)
Antibiotics, Antineoplastic/pharmacology , Apoptosis/drug effects , Doxorubicin/pharmacology , Hydrogen Peroxide/metabolism , Tumor Suppressor Protein p53/physiology , Animals , Antioxidants/pharmacology , Caspase 3 , Caspases/metabolism , Cattle , Cells, Cultured , Enzyme Activation , Glutathione Peroxidase/physiology , Male , Metalloporphyrins/pharmacology , Rats , Rats, Sprague-Dawley , Glutathione Peroxidase GPX1
8.
Proc Natl Acad Sci U S A ; 100(6): 3125-30, 2003 Mar 18.
Article in English | MEDLINE | ID: mdl-12624190

ABSTRACT

We examined iron nitrosylation of non-heme protein and enzymatic activity of the Fe-S cluster protein, aconitase, in acute cardiac allograft rejection. Heterotopic transplantation of donor hearts was performed in histocompatibility matched (isografts: Lewis --> Lewis) and mismatched (allografts: Wistar-Furth --> Lewis) rats. On postoperative days (POD) 4-6, Western blot analysis and immunohistochemistry revealed inducible nitric-oxide synthase (iNOS) protein in allografts but not isografts. EPR spectroscopy revealed background signals at g = 2.003 (for semiquinone) and g = 2.02 and g = 1.94 (for Fe-S cluster protein) in isografts and normal hearts. In contrast, in allografts on POD4, a new axial signal at g = 2.04 and g = 2.02 appeared that was attributed to the dinitrosyl-iron complex formed by nitrosylation of non-heme protein. Appearance of this signal occurred at or before significant nitrosylation of heme protein. Iron nitrosylation of non-heme protein was coincidental with decreases in the nonnitrosylated Fe-S cluster signal at g = 1.94. Aconitase enzyme activity was decreased to approximately 50% of that observed in isograft controls by POD4. Treatment with cyclosporine blocked the (i) elevation of plasma nitrate + nitrite, (ii) up-regulation of iNOS protein, (iii) decrease in Fe-S cluster EPR signal, (iv) formation of dinitrosyl-iron complexes, and (v) loss of aconitase enzyme activity. Formation of dinitrosyl-iron complexes and loss of aconitase activity within allografts also was inhibited by treatment of recipients with a selective iNOS inhibitor, l-N(6)-(1-iminoethyl)lysine. This report shows targeting of an important non-heme Fe-S cluster protein in acute solid organ transplant rejection.


Subject(s)
Graft Rejection/metabolism , Heart Transplantation/physiology , Lysine/analogs & derivatives , Nitric Oxide/metabolism , Nonheme Iron Proteins/metabolism , Aconitate Hydratase/metabolism , Animals , Cyclosporine/pharmacology , Electron Spin Resonance Spectroscopy , Enzyme Inhibitors/pharmacology , Graft Rejection/drug therapy , Graft Rejection/etiology , Heart Transplantation/adverse effects , Hemeproteins/chemistry , Hemeproteins/metabolism , Immunosuppressive Agents/pharmacology , Lysine/pharmacology , Nitric Oxide Synthase/antagonists & inhibitors , Nitric Oxide Synthase/metabolism , Nitric Oxide Synthase Type II , Nonheme Iron Proteins/chemistry , Rats , Rats, Inbred Lew , Rats, Inbred WF , Rats, Sprague-Dawley , Transplantation, Homologous , Transplantation, Isogeneic
9.
Free Radic Biol Med ; 33(7): 988, 2002 Oct 01.
Article in English | MEDLINE | ID: mdl-12361808

ABSTRACT

The cytoprotective effects of redox-active metalloporphyrins (e.g., FeTBAP and MnTBAP) were generally attributed to their ability to scavenge reactive oxygen and nitrogen species. In this study, we evaluated the pro- and antiapoptotic potentials of different metalloporphyrins containing iron, cobalt, zinc, and manganese in adult rat cardiomyocytes exposed to doxorubicin (DOX), an anticancer drug that forms superoxide and hydrogen peroxide via redox-cycling of DOX semiquinone in the presence of molecular oxygen. We used electron spin resonance/spin trapping and cytochrome c reduction to assess the scavenging of superoxide anion by metalloporphyrins. Superoxide anion was effectively scavenged by FeTBAP and MnTBAP but not by CoTBAP and ZnTBAP. FeTBAP efficiently scavenged H(2)O(2). Both CoTBAP and FeTBAP inhibited DOX-induced cardiomyocyte apoptosis. These findings implicate that mechanisms other than oxy-radical scavenging may account for their antiapoptotic property. In addition, CoTBAP and FeTBAP induced heme oxygenase-1 more potently than did MnTBAP and ZnTBAP. Inhibition of heme oxygenase abolished the protective effect of CoTBAP and reduced the protection by FeTBAP against DOX-induced cardiomyocyte apoptosis. We propose that metalloporphyrins can inhibit apoptosis either by inducing heme oxygenase-1 and antiapoptotic protein signaling or by scavenging reactive oxygen species.


Subject(s)
Apoptosis/physiology , Doxorubicin/pharmacology , Free Radical Scavengers/pharmacology , Heart/physiology , Heme Oxygenase (Decyclizing)/genetics , Metalloporphyrins/pharmacology , Reactive Oxygen Species/metabolism , Animals , Apoptosis/drug effects , Electron Spin Resonance Spectroscopy , Heart/drug effects , Heart Ventricles , Heme Oxygenase (Decyclizing)/biosynthesis , Heme Oxygenase-1 , Kinetics , Male , Muscle Cells/drug effects , Muscle Cells/metabolism , Rats , Rats, Sprague-Dawley , Superoxides/metabolism
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