Physical activity and pelvic floor muscle training during pregnancy: review of international recommendations.

Physical activity is associated with beneficial health effects for both mother and her future child, as well as the course of pregnancy. The aim of this review was the comparison of international guidelines with Polish recommendations. Data were collected from PubMed platform and international guidelines and narrowed to open access studies published between 1990-2023 in English, German and Polish. The existing literature shows an increase of interest in the impact of body activity during pregnancy and pelvic floor muscle training (PFMT). The recommendations move away from the more conservative approach, that previously suggested limitation of all exercise-related activities. Recently, The Polish Society of Gynecologists and Obstetricians and Polish Society of Sports Medicine announced a planned release of joint recommendations on physical activity during pregnancy and after childbirth, as well as the translation of the "Get Active Questionnaire for Pregnancy", a screening tool for pregnant women, doctors and midwives, developed in accordance with the recommendations of international gynecological societies.

The role of MPP1/p55 and its palmitoylation in resting state raft organization in HEL cells.

Here we show the crucial role of MPP1 in lateral membrane ordering/organization in HEL cells (derived from erythroid precursors). Biochemical analyses showed that inhibition of MPP1 palmitoylation or silencing of the MPP1 gene led to a dramatic decrease in the DRM fraction. This was accompanied by a reduction of membrane order as shown by fluorescence-lifetime imaging microscopy (FLIM) analyses. Furthermore, MPP1 knockdown significantly affects the activation of MAP-kinase signaling via raft-dependent RTK (receptor tyrosine kinase) receptors, indicating the importance of MPP1 for lateral membrane organization. In conclusion, palmitoylation of MPP1 appears to be at least one of the mechanisms controlling lateral organization of the erythroid cell membrane. Thus, this study, together with our recent results on erythrocytes, reported elsewhere (Lach et al., J. Biol. Chem., 2012, 287, 18974-18984), points to a new role for MPP1 and presents a novel linkage between membrane raft organization and protein palmitoylation.

Human DHHC proteins: a spotlight on the hidden player of palmitoylation.

Palmitoylation is one of the most common posttranslational lipid modifications of proteins and we now know quite a lot about it. However, the state of knowledge about the enzymes that catalyze this process is clearly insufficient. This review is focused on 23 human DHHC genes and their products - protein palmitoyltransferases. Here we describe mainly the structure and function of these proteins, but also, to a lesser degree, what the substrates of the enzymes are and whether they are related to various diseases. The main aim of this review was to catalogue existing information concerning the human DHHC family of genes/proteins, making them and their functions easier to understand.

[Analysis of labor methods after cesarean sections in the last 50 years]. / Analiza sposobów ukonczenia ciazy po przebytych cieciach cesarskich w ostatnim piecdziesiecioleciu.

The aim of the work was to analyze the incidence and changes in the labors after previous cesarean section in the last 50 years. The study was conducted on the basis of the labors documented in our Clinic between 1946 and 1996 year. Cesarean sections are more and more frequent in these years and the amount of the patients with previous cesarian section elevates. We observed the increase rate of repeat cesarean delivery in comparison to vaginal birth after cesarean section. The trial of vaginal labor after cesarean section is usually successful in young patients (under 25 years old), repeat cesareans is mainly observed in patients older than 35. The permanent decrease in the incidence of instrumental deliveries in comparison with cesarean section was observed. The trial of vaginal labor after cesarean section, after exclusion of contraindicatins, should be routine procedure decreasing the amount of cesarean sections.

[Evaluation of congenital infection risk on the basic of cytokines Il-1 beta, Il-6 and TNF alpha levels in blood serum and amniotic liquid in pregnant women and in umbilical blood of neonates]. / Ocena zagrozenia zakazeniem wrodzonym u noworodków w oparciu o wartosci cytokin: IL-1 beta, IL-6 i TNF alpha w surowicy krwi i plynie owodniowym ciezarnych oraz we krwi pepowinowej noworodków.

OBJECTIVE: The aim of the study was to evaluate congenital infection risk on the basic of cytokines IL-1 beta, IL-6 and TNF alpha levels in blood serum and amniotic liquid in pregnant women and in umbilical blood of neonates. MATERIALS AND METHODS: The study was carried out on 37 women in labor including 17 mothers with normal pregnancy (group A-test) and 20 suspected of intrauterine infection (group C). There have been indicated cytokines IL-1 beta, IL-6 and TNF alpha in amniotic liquid and in blood serum collected in the second stage of labor and in umbilical blood serum. The accepted procedure used in our study resulted from the assumption that the analysis of cytokines in amniotic liquid collected without amniocentesis was beneficial. RESULTS: It has been proved that among women in labor from group C there is a high concentration of IL-6 and TNF alpha in amniotic liquid that has statistic significance as far as markers for women with normal pregnancy are concerned (group A). Lower levels of IL-6 than in amniotic liquid but statistically meaningful in relation to the markers in group A have been detected in serum collected from pregnant women and in the umbilical blood of the neonates. Among 18 neonates delivered by mothers from group C the symptoms of congenital infection have appeared. CONCLUSION: Extending diagnostics of congenital infection on the basis of the analyzed cytokines, mainly IL-6 in the second stage of labor, is of prognostic significance.