ABSTRACT
The paper deals with the results of analysis of 219 strains of Klebsiella using 12 tests--fermentation of adonite, asparaginic acid, sodium citrate, dulcite, d-tartrate, glutamic acid, inosite, L-proline, sodium malonate; reactions with methyl red, Foges-Proscauer, with 5-ASA. A scheme for determining fermentovars of Klebsiella is suggested which includes the tests--fermentation of adonite, dulcite, d-tartrate, glutamic acid; color reaction with 5-ASA. The groups of Klebsiella different in origin are characterized by nonhomogeneous distribution of different fermentovars. The suggested method of biochemical labelling may be one of the basic ones in complex typing of Klebsiella.
Subject(s)
Bacterial Typing Techniques , Klebsiella/classification , Culture Media , Humans , Klebsiella/enzymology , Species SpecificityABSTRACT
Results of bacteriocin typing of 196 strains of the Klebsiella genus are presented. They are typified by their sensitivity to bacteriocins and by their production using colicinogenic and indicating strains from collection of P. Fredericq [correction of Frederick], D. G. Kudlai, N. I. Koshanova as well as klebocinogenic K-type cultures of Klebsiella previously suggested by the authors. Investigation results have shown sufficient stability of a bacteriocinotype of the cultures confirmed by the population analysis. It is concluded that bacteriocin typing may be recommended as an additional method in epidemiological labelling of Klebsiella cultures.
Subject(s)
Bacterial Typing Techniques , Klebsiella pneumoniae/classification , Bacteriocins/biosynthesis , Bacteriocins/classification , Bacteriocins/pharmacology , Colicins/pharmacology , Humans , Klebsiella pneumoniae/drug effects , Klebsiella pneumoniae/isolation & purification , Klebsiella pneumoniae/metabolism , Microbial Sensitivity TestsABSTRACT
A phenomenon of bacteriocinogeny is studied in 83 K-type strains of Klebsiella. Physicochemical (permeability through cellophane, thermo-resistance, diffusion rate in agar, inactivation by trypsin, induction by UV irradiation) and biological (activity spectrum, specificity) properties are studied. Eight preparations of klebocins are examined by electron microscopy. Conclusions are made on the possibility to use klebocinogenic and indicating K-type cultures of Klebsiella for bacteriocin typing of clinical isolates of these microbes.
Subject(s)
Bacteriocins/biosynthesis , Klebsiella pneumoniae/metabolism , Bacterial Typing Techniques , Bacteriocins/isolation & purification , Bacteriocins/pharmacology , Chemical Phenomena , Chemistry, Physical , Drug Resistance, Microbial , Klebsiella pneumoniae/classification , Klebsiella pneumoniae/drug effects , Microbial Sensitivity Tests , Microscopy, Electron , Mutation/drug effects , Species Specificity , Ultraviolet RaysABSTRACT
Hemolytic activities of Klebsiella-237 strains of various origins were measured in solid nutrient media by three methods making use of six different agar bases for the preparation of blood nutrient media. The authors analyze the agar bases, discuss the advantages and shortcomings of all the methods used with consideration for the purpose of the investigation. The findings evidence a higher hemolytic activity in Klebsiella isolated from patients as against the hemolytic activities of reference strains.
Subject(s)
Hemolysis/physiology , Klebsiella/physiology , Animals , Culture Media , Evaluation Studies as Topic , Humans , In Vitro Techniques , RabbitsABSTRACT
The technique of making paper disks with Klebsiella bacteriocins is described, as is the method for improving the stability of bacteriocin preparations. The authors have developed a method for bacterial bacteriocin typing, facilitating the work and taking but 4-5 hrs. The results of klebocin typing of Klebsiella-237 strains with the use of the developed technique are presented.