ABSTRACT
Embryo assessment and selection is a critical step in an in vitro fertilization (IVF) procedure. Current embryo assessment approaches such as manual microscopy analysis done by embryologists or semi-automated time-lapse imaging systems are highly subjective, time-consuming, or expensive. Availability of cost-effective and easy-to-use hardware and software for embryo image data acquisition and analysis can significantly empower embryologists towards more efficient clinical decisions both in resource-limited and resource-rich settings. Here, we report the development of two inexpensive (<$100 and <$5) and automated imaging platforms that utilize advances in artificial intelligence (AI) for rapid, reliable, and accurate evaluations of embryo morphological qualities. Using a layered learning approach, we have shown that network models pre-trained with high quality embryo image data can be re-trained using data recorded on such low-cost, portable optical systems for embryo assessment and classification when relatively low-resolution image data are used. Using two test sets of 272 and 319 embryo images recorded on the reported stand-alone and smartphone optical systems, we were able to classify embryos based on their cell morphology with >90% accuracy.
Subject(s)
Blastocyst , Deep Learning , Embryonic Development , Image Processing, Computer-Assisted , Time-Lapse Imaging , Fertilization in Vitro , HumansABSTRACT
The ability to accurately predict ovulation at-home using low-cost point-of-care diagnostics can be of significant help for couples who prefer natural family planning. Detecting ovulation-specific hormones in urine samples and monitoring basal body temperature are the current commonly home-based methods used for ovulation detection; however, these methods, relatively, are expensive for prolonged use and the results are difficult to comprehend. Here, we report a smartphone-based point-of-care device for automated ovulation testing using artificial intelligence (AI) by detecting fern patterns in a small volume (<100 µL) of saliva that is air-dried on a microfluidic device. We evaluated the performance of the device using artificial saliva and human saliva samples and observed that the device showed >99% accuracy in effectively predicting ovulation.