Biomedical Optics Express Feature Issue Introduction: Optical Manipulation and Its Applications (OMA) 2023.

The feature issue of Biomedical Optics Express presents studies that were the focus of the Optical Manipulation and its Applications (OMA) meeting that was held on 24 - 27 April 2022 in Vancouver, Canada.

Asymmetric split-ring plasmonic nanostructures for the optical sensing of Escherichia coli.

Strategies for in-liquid micro-organism detection are crucial for the clinical and pharmaceutical industries. While Raman spectroscopy is a promising label-free technique for micro-organism detection, it remains challenging due to the weak bacterial Raman signals. In this work, we exploit the unique electromagnetic properties of metamaterials to identify bacterial components in liquid using an array of Fano-resonant metamolecules. This Fano-enhanced Raman scattering (FERS) platform is designed to exhibit a Fano resonance close to the protein amide group fingerprint around 6030 nm. Raman signatures of Escherichia coli were recorded at several locations on the metamaterial under off-resonance laser excitation at 530 nm, where the photodamage effect is minimized. As the sizes of the Escherichia coli are comparable to the micro-gaps i.e, 0.41 µm, of the metamaterials, its local immobilisation leads to an increase in the Raman sensitivity. We also observed that the time-dependent FERS signal related to bacterial amide peaks increased during the bacteria's mid-exponential phase while it decreased during the stationary phase. This work provides a new set of opportunities for developing ultrasensitive FERS platforms suitable for large-scale applications and could be particularly useful for diagnostics and environmental studies at off-resonance excitation.

Enabling Self-Induced Back-Action Trapping of Gold Nanoparticles in Metamaterial Plasmonic Tweezers.

The pursuit for efficient nanoparticle trapping with low powers has led to optical tweezers technology moving from the conventional free-space configuration to advanced plasmonic systems. However, trapping nanoparticles smaller than 10 nm still remains a challenge even for plasmonic tweezers. Proper nanocavity design and excitation has given rise to the self-induced back-action (SIBA) effect offering enhanced trap stiffness with decreased laser power. In this work, we investigate the SIBA effect in metamaterial tweezers and its synergy with the exhibited Fano resonance. We demonstrate stable trapping of 20 nm gold particles with trap stiffnesses as high as 4.18 ± 0.2 (fN/nm)/(mW/µm2) and very low excitation intensity. Simulations reveal the existence of two different groups of hotspots on the plasmonic array. The two hotspots exhibit tunable trap stiffnesses, a unique feature that can allow for sorting of particles and biological molecules based on their characteristics.

Analysis of small microplastics in coastal surface water samples of the subtropical island of Okinawa, Japan.

Marine plastic debris is widely recognized as a global environmental issue. Small microplastic particles, with an upper size limit of 20 µm, have been identified as having the highest potential for causing damage to marine ecosystems. Having accurate methods for quantifying the abundance of such particles in a natural environment is essential for defining the extent of the problem they pose. Using an optical micro-Raman tweezers setup, we have identified the composition of particles trapped in marine aggregates collected from the coastal surface waters around the subtropical island of Okinawa. Chemical composition analysis at the single-particle level indicates dominance by low-density polyethylene, which accounted for 75% of the small microplastics analysed. The smallest microplastics identified were (2.53 ± 0.85) µm polystyrene. Our results show the occurrence of plastics at all test sites, with the highest concentration in areas with high human activities. We also observed additional Raman peaks on the plastics spectrum with decreasing debris size which could be related to structural modification due to weathering or embedding in organic matter. By identifying small microplastics at the single-particle level, we obtain some indication on their dispersion in the ocean which could be useful for future studies on their potential impact on marine biodiversity.

Fast and efficient nanoparticle trapping using plasmonic connected nanoring apertures.

The manipulation of microparticles using optical forces has led to many applications in the life and physical sciences. To extend optical trapping towards the nano-regime, in this work we demonstrate trapping of single nanoparticles in arrays of plasmonic coaxial nano-apertures with various inner disk sizes and theoretically estimate the associated forces. A high normalized experimental trap stiffness of 3.50 fN nm-1 mW-1 µm-2 for 20 nm polystyrene particles is observed for an optimum design of 149 nm for the nanodisk diameter at a trapping wavelength of 980 nm. Theoretical simulations are used to interpret the enhancement of the observed trap stiffness. A quick particle trapping time of less than 8  s is obtained at a concentration of 14 × 1011 particles ml-1 with low incident laser intensity of 0.59 mW µm-2. This good trapping performance with fast delivery of nanoparticles to multiple trapping sites emerges from a combination of the enhanced electromagnetic near-field and spatial temperature increase. This work has applications in nanoparticle delivery and trapping with high accuracy, and bridges the gap between optical manipulation and nanofluidics.

Fano-Resonant, Asymmetric, Metamaterial-Assisted Tweezers for Single Nanoparticle Trapping.

Plasmonic nanostructures overcome Abbe's diffraction limit to create strong gradient electric fields, enabling efficient optical trapping of nanoparticles. However, it remains challenging to achieve stable trapping with low incident laser intensity. Here, we demonstrate Fano resonance-assisted plasmonic optical tweezers for single nanoparticle trapping in an array of asymmetrical split nanoapertures on a 50 nm gold thin film. A large normalized trap stiffness of 8.65 fN/nm/mW for 20 nm polystyrene particles at a near-resonance trapping wavelength of 930 nm was achieved. The trap stiffness on-resonance is enhanced by a factor of 63 compared to that of off-resonance due to the ultrasmall mode volume, enabling large near-field strengths and a cavity effect contribution. These results facilitate trapping with low incident laser intensity, thereby providing new options for studying transition paths of single molecules such as proteins.