A facility for X-ray diffraction in magnetic fields up to 25 T and temperatures between 15 and 295 K.

A facility for X-ray diffraction has been developed at the National High Magnetic Field Laboratory. It brings diffraction capability to the 25 T Florida split coil magnet and implements temperature control in a range of 15-295 K using a cold finger helium cryostat. This instrument represents an alternative to pulsed magnetic field systems, and it exceeds the static magnetic fields currently available at synchrotron facilities. Magnetic field compatibility of an X-ray source and detectors with the sizable magnetic fringe fields emanating from the magnet constrained the design of the diffractometer.

[A study of interaction of 7-aminoactinomycin D with DNA by fluorescence correlation spectroscopy]. / Issledovanie vzaimodeistviia 7-aminoaktinomitsina D s DNK metodom fluorestsentnoi korreliatsionnoi spektroskopii.

The investigation of interaction of 7-aminoactinomycin D with DNA is hampered at nanomolar concentrations by low quantum yield of the dye fluorescence and high adsorbability of the dye. It was found that, instead of the increase in the fluorescence of 7-aminoactinomycin D after binding with DNA, a decrease in the fluorescence takes place, because 7-aminoactinomycin D complexed with DNA exists in two states: photochemically active and inactive. The presence of the photochemically active state of 7-aminoactinomycin D causes an apparent increase in the diffusion coefficient upon embedding of 7-aminoactinomycin D into DNA. The data obtained allow one to state that 7-aminoactinomycin D: (1) forms dimers at micromolar concentration, (2) has two specific photodestruction times, and (3) binds with DNA more effectively than actinomycin D. Polarization measurements made it possible to estimate numerically the adsorption of 7-aminoactinomycin D on the walls of the measuring cell.

Angle-resolved mapping of the fermi velocity in a quasi-two-dimensional organic conductor.

We demonstrate a new method for determining the Fermi velocity in quasi-two-dimensional (Q2D) conductors. Application of a magnetic field parallel to the conducting layers results in periodic open orbit quasiparticle trajectories along the Q2D Fermi surface. Averaging of this motion over the Fermi surface leads to a resonance in the interlayer microwave conductivity. The resonance frequency is simply related to the extremal value of the Fermi velocity perpendicular to the applied field. Thus, angle dependent microwave studies enable a complete mapping of the in-plane Fermi velocity. We illustrate the applicability of this method for the highly 2D organic conductor kappa-(BEDT-TTF)2I3.

[Vibrational structure of absorption spectra of water in visible region]. / O strukture kolebatel'nykh spektrov pogloshcheniia vody v vidimom diapazone.

The results of the study of the temperature dependence of water absorption spectra in the visible region and in the region of vibrational overtones are discussed.

[The role of intracellular Ca(2+) pools in the regulation of protoplast volume. Effect of red light on the Ca(2+) mobilization in cytoplasm of Arabidopsis cells]. / Rol' vnutrikletochnykh pulov Ca(2+) v reguliatsii ob''ema protoplastov. Vliianie krasnogo sveta na mobilizatsiiu Ca(2+) v tsitoplazme kletok Arabidopsis.

The changes in cytosol Ca2+ concentration associated with the shrinkage of Arabidopsis cells induced by the inhibitor of Ca(2+)-ATPase, cyclopiazonic acid and the Ca2+ ionophore ionomycin were monitored using the fluorescence of Ca(2+)-sensitive probe chlortetracycline hydrochloride. It was found that these compounds elicited a substantial decrease in fluorescence intensity closely associated with Ca(2+)-release from the intracellular stores to the cytoplasm. The release of Ca2+ from the intracellular depots was accompanied by decrease of plant cell volume. Thapsigargin and 2,5'-ditert-butyl-1,4-benzohydroquinone (highly specific inhibitors of Ca(2+)-ATPase of endoplasmic reticulum) resulted in much weaker changes than cyclopiazonic acid did. It was also found with the help of the same technique that red light (lambda = 660 nm) illumination induced a similar Ca2+ release from the intracellular stores. Moreover, the amplitudes of light-induced fluorescence responses registered in mutant plants differing in the content of phytochrome A (phyAOX) and phytochrome B (phyBOX) were much higher than those registered in wild-type of Arabidopsis.

Magnetic field dependence of the superconducting gap and the pseudogap in Bi2212 and HgBr2-Bi2212, studied by intrinsic tunneling spectroscopy.

Intrinsic tunneling spectroscopy in high magnetic field (H) is used for a direct test of superconducting features in the quasiparticle density of states of pure Bi2212 and intercalated HgBr2-Bi2212 high- T(c) superconductors. We were able to distinguish with great clarity two coexisting gaps: (i) the superconducting gap, which closes as H-->H(c2)(T), and (ii) the c-axis pseudogap, which does not change either with H or with T. Strikingly different H dependencies, together with previously observed different temperature dependencies of the two gaps, speak against a superconducting origin of the pseudogap.