Growth status and age at peak height velocity among youth participants in several sports: the Cracow longitudinal study.

BACKGROUND: Studies addressing age at peak height velocity (PHV) in longitudinal samples of participants in sports are relatively limited. PURPOSE: To compare the growth status and estimated ages at PHV of longitudinal samples girls and boys active in sport with peers not active in sport, and to compare estimated ages at PHV among longitudinal samples of Polish youth active in sport. METHODS: Records from the Cracow Longitudinal Study, which measured youth annually from 8 to 18 years, were screened to identify individuals regularly active in sport. Participants in athletics (22 girls, 10 boys), soccer (12 boys), and other team (6 girls, 7 boys) and individual (6 girls, 9 boys) sports were identified; 107 girls and 172 boys were not active in sport. Heights and weights of participants in sports and non-involved peers were compared. Longitudinal height records of individuals were fit with the SITAR model to estimate age at PHV. Ages at PHV of boys and girls active in sport and peers not active in sports were compared with sex-specific ANOVAs. RESULTS: Ages at PHV of boys participating in athletics and soccer were similar to age at PHV of boys not active in sport, while ages at PHV of boys in other team sports (basketball, volleyball, handball) and individual sports (skiing, gymnastics, acrobatics) were, respectively, slightly earlier and later. Among girls, age at PHV of participants in team sports (basketball, netball) was earlier, while ages at PHV among participants in athletics and other individual sports (equestrian, acrobatics, shooting) were slightly later compared to non-athlete peers. CONCLUSION: Ages at PHV varied among participants in different sports and were consistent with estimates in other longitudinal samples of Polish youth athletes.

Age at peak height velocity in Polish adolescents: Effect of socioeconomic factors.

Age at peak height velocity (APHV) is an indicator of maturity timing which is applicable to both sexes, and which is influenced by environmental factors. The objective of this study was to assess variation in APHV associated with several indicators of socioeconomic status (SES) in a longitudinal sample of Polish adolescents. The sample included 739 boys born in 1983 and followed annually from 12 to 16 years, and 597 girls born in 1985 and followed annually from 9 to 13 years. The height records were fitted with the SITAR model to estimate APHV. SES was estimated using principal component analysis of indicators of familial status based on parental education, family size, living conditions and household possessions. Statistical analyses included analysis of variance (one-way for general SES and three-way for parental education and family size) and Tukey post-hoc tests for unequal samples. General SES (p <.001) and family size (p < .05) significantly influenced APHV among boys, while only maternal education (p < .05) significantly influenced APHV among girls. Among youth from families of higher SES, as defined by the respective indicators, APHV was attained significantly earlier, on average, than in peers from families of lower SES. Overall, the results showed a sex-dependent effect of SES on APHV, and highlighted the influence of favorable socioeconomic conditions for optimal growth and maturation during adolescence.

Ages at peak height velocity in male soccer players 11-16 years: relationships with skeletal age and comparisons among longitudinal studies.

Estimated ages at take-off (TO) and at peak height velocity (PHV) based on two models and maturity status based upon age at PHV and skeletal age (SA) were compared in a longitudinal sample of male soccer players. In addition, estimated ages at PHV in 13 longitudinal samples of soccer players were compared. The longitudinal height records of 58 players of European ancestry, measured annually on four or five occasions between 11 and 16 years, were modeled with Superimposition by Translation and Rotation (SITAR) and Functional Principal Component Analysis (FPCA) to estimate ages at TO and PHV. SAs were assessed with the Fels method. Ages at PHV in 13 longitudinal samples of soccer players (Europe 7, Japan 6) were evaluated with meta-analysis. Estimated ages at TO, 11.2 ± 0.8 (SITAR) and 11.0 ± 0.8 (FCPA) years, and at PHV, 13.6 ± 0.9 (SITAR) and 13.7 ± 0.0 (FCPA) years, were similar. An earlier age at PHV was associated with advanced skeletal maturity status (rho = -0.77 at ~14 years). Ages at PHV among European players indicated a north (later) - south (earlier) gradient, and were later than ages at PHV among Japanese players. In summary, ages at TO and PHV were similar with SITAR and FPCA, and ages at PHV were most strongly correlated with SA at ~14 years. Mean ages at PHV showed a north-south gradient among European samples, and were later compared to Japanese samples.

Porotic hyperostosis and cribra orbitalia in samples from the Late Hillfort Period sites at Detkovice - Za zahradama (district Prostejov) and Vídenská street (district Brno), Czech Republic.

Porotic hyperostosis and cribra orbitalia are pathological changes occurring on the human skull. These changes were observed and evaluated on skeletal remains from Detkovice - Za zahradama and Vídenská Street in Brno; both sites are dated back to the 10th to 12th centuries AD. A total of 605 subjects were assessed for age, sex, and the above-stated pathologies using standard methods. The influence of age and sex on the occurrence of these pathologies was examined statistically. Results indicated that at the site of Detkovice - Za zahradama, porotic hyperostosis, and cribra orbitalia do not depend on sex or age. However, at Vídenská Street in Brno, these pathologies do not depend on sex, but they depend on age so a higher incidence of pathologies in juveniles is observed. Differences between both sites could be caused by different numbers of evaluated individuals or different state of preservation of skeletal remains. The aetiology of the origin of these pathologies could not be determined by the methodology used here, but with the prevailing lower age of pathological subjects, a lack of nutrition with consequent absence of iron and developing anaemia might be the cause. Based on our statistical data, we can observe that the pathologies we studied occur more frequently in children older than newborns and younger infants. This may indicate that these studied pathologies arise only during the lifetime of the individual and do not have a prenatal occurrence.

Cortisol in deciduous tooth tissues: A potential metric for assessing stress exposure in archaeological and living populations.

OBJECTIVE: Cortisol is a glucocorticoid hormone produced by the hypothalamic-pituitary-adrenal axis that is regularly assessed in modern human and non-human populations in saliva, blood, and hair as a measure of stress exposure and stress reactivity. While recent research has detected cortisol concentrations in modern and archaeological permanent dental tissues, the present study assessed human primary (deciduous) teeth for cortisol concentrations. MATERIALS AND METHODS: Fifty-one dentine and enamel samples from nine modern and 10 archaeological deciduous teeth were analyzed for cortisol concentrations via enzyme-linked immunosorbent assay (ELISA). RESULTS: Detectable concentrations of cortisol were identified in 15 (of 32) dentine and 8 (of 19) enamel samples coming from modern and archaeological deciduous teeth. CONCLUSIONS: This study is the first known analysis of cortisol from deciduous dental tissues, demonstrating the potential to identify measurable concentrations. SIGNIFICANCE: The ability to analyze deciduous teeth is integral to developing dental cortisol methods with multiple potential future applications, including research on the biological embedding of stress in the skeleton. This study marks a key step in a larger research program to study stress in primary dentition from living and archaeological populations. LIMITATIONS: Multiple samples generated cortisol values that were not detectable with ELISA. Minimum quantities of tissue may be required to generate detectable levels of cortisol. SUGGESTIONS FOR FURTHER RESEARCH: Future research should include larger sample sizes and consideration of intrinsic biological and extrinsic preservation factors on dental cortisol. Further method validation and alternative methods for assessing dental cortisol are needed.

Within-family intergenerational trends in age at menarche relative to political changes in Czechoslovakia after World War II.

OBJECTIVES: The aim of the study was to examine a secular trend in age at menarche (AAM) in the former Czechoslovak (and descendant Slovak and Czech) population in relation to its large-scale political and social events taking place after World War II. METHODS: The study included 211 women aged 18-30 (born during 1984-1998), and their relatives: mothers, sisters, and grandmothers, yielding a total of 421 women. Changes in retrospectively recalled AAM between the three generations of women (oldest-grandmothers, middle-mothers, and youngest-daughters) were studied in pairwise comparisons. Relationships between AAM and the birth/conception date were analyzed relative to three events in the post-WWII Czechoslovakia (1948, 1968, and 1989). RESULTS: AAM was the highest in the oldest generation, slightly lower in the middle generation and the lowest in the youngest generation. Mixed-Effect Model showed statistically significant interaction between the date of conception, historical events, and the period before and after the event. CONCLUSIONS: The recorded decline in AAM is congruent with secular trends reported in the literature. However, the decreasing trend was not linear and included an increase in AAM in women conceived within the five-year period after the invasion of Czechoslovakia by communist armies in 1968.

Radioulnar contrasts in fingerprint ridge counts: Searching for dermatoglyphic markers of early sex development.

OBJECTIVES: Using prenatally fixed dermatoglyphics features as markers of prenatal sex development is limited due to insufficient knowledge on their sex differences. This study aims to examine more thoroughly sex differences in radioulnar contrasts. METHODS: Fingerprints of 360 females and 331 males from four samples of different ethnic backgrounds (Czechs, Slovaks, Vietnamese and Lusatian Sorbs) were studied. On both hands, finger ridge counts were recorded, and all possible radioulnar contrasts were computed as a difference between ridge count at a radial position minus ridge count at a respective ulnar position on the hand. Radioulnar contrasts with population-congruent and numerically large dimorphism were selected and the dimorphism of the selected radioulnar contrasts was then tested using nonparametric analysis of variance. RESULTS: Greater dimorphism of radioulnar contrasts occurred on the right hand than on the left hand. Population congruent direction and relatively strong dimorphism (Cohen's d greater than 0.3) was found in six radioulnar contrasts on the right hand, all of which involved the radial ridge count of the 2nd finger. Of these, the highest average dimorphism was observed for the difference between the radial ridge count of the 2nd finger and the ulnar ridge count of the 4th finger (2r4u contrast), where the average effect size from all four population samples was comparable to a published average effect size of the 2D:4D finger length ratio. CONCLUSION: We propose that 2r4u contrast of ridge counts could serve as a marker of prenatal sexual development targeting a temporally narrow developmental window.

The strong impact of maternal marital status on birth body size before and during the Second World War in Poznan district, Poland.

OBJECTIVES: The study aims to examine whether maternal socio-economic status, represented by marital status and the place of residence, affected birth body size (BBS) of babies in the pre-war period and during the WWII. METHODS: The dataset consisted of 8934 unique individual information items on mothers and deliveries collected for two birth cohorts: born before (1934, 1935, 1936, and 1937) and during the WWII (1941, 1942, 1943 and 1944). BBS (weight, length, BMI) was compared according to mother's marital status and her place of residence in two cohorts separately. (ART)ANOVA was applied to test the effect of a child's sex, maternal marital status (MMS), and maternal place of residence (MPR) on birth weight/length/BMI of babies born alive before and during the WWII. RESULTS: Babies with greater BBS were born to married mothers than to single ones. This pattern applied to pre-war and to the WWII cohort. In both pre-war and the WWII cohorts the MMS had the strongest impact on BBS. The effect of mother's place of residence on BBS was observed in the pre-war cohort only. CONCLUSIONS: Marital status could have acted through economic and social factors, level of psychosocial stress and support, social (in)stability. In the pre-war period, the place of residence much more reflected socio-economic differences between localities. Marginal economic, health and nutritional conditions associated with the WWII affected mothers regardless of the size of their place of residence.

Estimating Growth in Height from Limited Longitudinal Growth Data Using Full-Curves Training Dataset: A Comparison of Two Procedures of Curve Optimization-Functional Principal Component Analysis and SITAR.

A variety of models are available for the estimation of parameters of the human growth curve. Several have been widely and successfully used with longitudinal data that are reasonably complete. On the other hand, the modeling of data for a limited number of observation points is problematic and requires the interpolation of the interval between points and often an extrapolation of the growth trajectory beyond the range of empirical limits (prediction). This study tested a new approach for fitting a relatively limited number of longitudinal data using the normal variation of human empirical growth curves. First, functional principal components analysis was done for curve phase and amplitude using complete and dense data sets for a reference sample (Brno Growth Study). Subsequently, artificial curves were generated with a combination of 12 of the principal components and applied for fitting to the newly analyzed data with the Levenberg-Marquardt optimization algorithm. The approach was tested on seven 5-points/year longitudinal data samples of adolescents extracted from the reference sample. The samples differed in their distance from the mean age at peak velocity for the sample and were tested by a permutation leave-one-out approach. The results indicated the potential of this method for growth modeling as a user-friendly application for practical applications in pediatrics, auxology and youth sport.

Observed and predicted ages at peak height velocity in soccer players.

The purpose of the study was to evaluate predicted maturity offset (time before age at PHV) and age at PHV (chronological age [CA] minus maturity offset) in a longitudinal sample of 58 under-13 club level soccer players in central Portugal for whom ages at PHV were estimated with the SITAR model. Two maturity offset prediction equations were applied: the original equation which requires CA sitting height, estimated leg length, height and weight, and a modified equation which requires CA and height. Predicted maturity offset increased, on average, with CA at prediction throughout the age range considered, while variation in predicted maturity offset and ages at PHV within CA groups was considerably reduced compared to variation in observed ages at offset and at PHV. Predicted maturity offset and ages at PHV were consistently later than observed maturity offset and age at PHV among early maturing players, and earlier than observed in late maturing players. Both predicted offset and ages at PHV with the two equations were, on average, later than observed among players maturing on time. Intra-individual variation in predicted ages at PHV with each equation was considerable. The results for soccer players were consistent with similar studies in the general population and two recent longitudinal studies of soccer players. The results question the utility of predicted maturity offset and age at PHV as valid indicators of maturity timing and status.

Prediction of maturity offset and age at peak height velocity in a longitudinal series of boys and girls.

BACKGROUND: Predicted maturity offset, defined as time before peak height velocity (PHV) is increasingly used as an indicator of maturity status in studies of physical activity, fitness, and sport. OBJECTIVE: To validate maturity offset prediction equations in longitudinal samples of boys and girls. METHODS: The original and modified maturity offset prediction equations were applied to serial data for 266 boys (8-17 years) and 147 girls (8-16 years) from the Cracow Growth Study. Actual age at PHV for each youngster was estimated with the SITAR protocol. In addition to maturity offset, the difference between CA at prediction and maturity offset provided an estimate of predicted age at PHV. RESULTS: Predicted maturity offset and age at PHV increased, on average, with CA at prediction. Variation in predictions was reduced compared to that in observed ages at offset and at PHV, and was more apparent with the modified equations. Relatively few predicted ages at PHV approximated observed age at PHV in early and late maturing youth of both sexes; predictions were later than observed among the former, and earlier than observed among the latter. CONCLUSION: Predicted maturity offset and ages at PHV with the original and modified equations increase with CA at prediction, have reduced variation, and have major limitations with early and late maturing boys and girls.

Body size at birth in babies born during World War II: The evidence from Poland.

OBJECTIVES: The objective of the study was to determine whether exposure of pregnant women to stresses caused by World War II (WWII) negatively affected pregnancy and perinatal outcomes. METHODS: Individual medical documents deposited in the Gynaecology and Obstetrics Clinic of Medical University, Poznan (1934-1943; N = 7058) were evaluated. These were divided into two birth cohorts: before WWII and during it. Frequency tables were constructed for the numbers of pregnancy outcomes: miscarriages, stillbirths, live births, and neonatal deaths, according to the period of birth and sex of a child. The numbers of recorded days were standardized and the numbers of cases per day were computed. Statistical differences in the averages (medians) between periods and years under study were tested. Birth weight, length, and body mass index (BMI) were compared according to the periods related to WWII. RESULTS: Significant differences in proportions of males, females, and subjects with unknown sex were found between the periods: a higher proportion of males and different structure of/within negative outcomes were found during WWII. Children born during WWII were heavier and longer than those born before it. CONCLUSIONS: As an explanation, adverse conditions of WWII, related to the psychological stress and food shortages, could have influenced greater elimination of fetuses and neonates of male sex during pregnancy and shortly after delivery. Higher average body size in newborns recorded during WWI could be explained by a hidden process of increased early prenatal mortality of weaker individuals, differences in average gestation length between the periods, differences in parity, or some undocumented differences in social/ethnic composition of the sample.

Family effects on the digit ratio (2D:4D): The role of the interbirth interval.

OBJECTIVES: Family represents the closest social environment that immediately affects human ontogeny from an early prenatal period. This study aimed to assess sibship influences on the second-to-fourth digit length ratio (2D:4D ratio). METHODS: The source sample represented 329 children aged 6.5 to 15.8 years (Czech Republic), including a subsample of 75 sibling pairs. A combination of (a) between-family design (cross-sectional sample) and (b) within-family design (pairs of siblings) was used to study the effect of family variables and interbirth interval (IBI) on the 2D:4D ratio. RESULTS: Birth order, number of siblings (except for younger sisters), and sex ratio in siblings were significantly related to the 2D:4D ratio on the right, left, or both hands. At the same time, the relationships were opposite in males and females for birth order, number of older brothers and number of children in the family--increasing values in these variables increased digit ratio in males but decreased digit ratio in females. Mean difference in 2D:4D ratio (DIFF) within pairs (older minus younger sibling) differed from zero only in the "older sister--younger brother" group, where DIFF did not depend on IBI. On the contrary, in remaining pair types the DIFF tended to change with IBI, the strongest in the "older brother--younger sister" pairs. CONCLUSIONS: Family variables, especially number of older brothers, should be considered as important confounding factors in 2D:4D ratio studies. However, the effect of these variables might be modified by IBI.

Sex differences in frequencies of dermatoglyphic patterns by individual fingers.

Background: The size of sex differences in dermatoglyphic features and their inter-population differences remains a subject of debate. Combining fingers in traditional dermatoglyphic methodology and omitting finger-specific variations might be a cause for uncertainty.Aim: To compare sex differences in whorl frequencies between fingers.Subjects and methods: Using meta-analytical methods, the authors studied sex differences in frequencies of whorls (log Odd Ratios) for each finger separately, including their heterogeneities (between-samples variance). The dataset of 204 population samples was extracted from published dermatoglyphic studies.Results: Aggregated effects of sex differences were significant in all fingers, except for the left 1st finger. Sex differences were higher in the right hand and increased from radial to ulnar fingers. Apart from the right 1st and 3rd fingers, heterogeneities were small and literally zero in the right 4th finger.Conclusion: Higher sex differences in ulnar fingers and the lack of interpopulation differences all over the world in the 4th finger might be caused by a stronger influence of genetic and/or hormonal factors on dermatoglyphic development of the ulnar side of the hand. It is suggested that future studies, when applying dermatoglyphic traits as markers of prenatal environment, use traits by individual fingers or their relationships within the hand.

Determination of Milk Products in Ceramic Vessels of Corded Ware Culture from a Late Eneolithic Burial.

In this study, a soil from two ceramic vessels belonging to Corded Ware culture, 2707⁻2571 B.C., found in a cremation grave discovered in Central Moravia, Czech Republic, was analyzed using matrix-assisted laser desorption/ionization⁻mass spectrometry (MALDI⁻MS) combined with advanced statistical treatment (principal component analysis, PCA, and orthogonal projection to latent structures discriminant analysis, OPLS-DA) and by enzyme-linked immunosorbent assay (ELISA). MALDI⁻MS revealed the presence of triacylglycerols in both vessels. This analytical technique was used for the analysis of the soil content from archaeological ceramic vessels for the first time. Targeted ELISA experiments consequently proved the presence of milk proteins in both ceramic vessels. These results represent the first direct evidence of the use of milk or dairy products in the Eneolithic period in Moravian Corded Ware Culture and help to better understand the diet habits and living conditions of Eneolithic populations in Central Europe.

Mitochondrial genomes reveal an east to west cline of steppe ancestry in Corded Ware populations.

From around 4,000 to 2,000 BC the forest-steppe north-western Pontic region was occupied by people who shared a nomadic lifestyle, pastoral economy and barrow burial rituals. It has been shown that these groups, especially those associated with the Yamnaya culture, played an important role in shaping the gene pool of Bronze Age Europeans, which extends into present-day patterns of genetic variation in Europe. Although the genetic impact of these migrations from the forest-steppe Pontic region into central Europe have previously been addressed in several studies, the contribution of mitochondrial lineages to the people associated with the Corded Ware culture in the eastern part of the North European Plain remains contentious. In this study, we present mitochondrial genomes from 23 Late Eneolithic and Bronze Age individuals, including representatives of the north-western Pontic region and the Corded Ware culture from the eastern part of the North European Plain. We identified, for the first time in ancient populations, the rare mitochondrial haplogroup X4 in two Bronze Age Catacomb culture-associated individuals. Genetic similarity analyses show close maternal genetic affinities between populations associated with both eastern and Baltic Corded Ware culture, and the Yamnaya horizon, in contrast to larger genetic differentiation between populations associated with western Corded Ware culture and the Yamnaya horizon. This indicates that females with steppe ancestry contributed to the formation of populations associated with the eastern Corded Ware culture while more local people, likely of Neolithic farmer ancestry, contributed to the formation of populations associated with western Corded Ware culture.

Meeting Report: Growth and Social Environment. Proceedings of the 25th Aschauer Soiree, held at Krobielowice, Poland, November 18th 2017.

Twenty-two scientists met at Krobielowice, Poland, to discuss the impact of the social environment, spatial proximity, migration, poverty, but also psychological factors such as body perception and satisfaction, and social stressors such as elite sports, and teenage pregnancies, on child and adolescent growth. The data analysis included linear mixed effects models with different random effects, Monte Carlo analyses, and network simulations. The work stressed the importance of the peer group, but also included historic material, some considerations about body proportions, and growth in chronic liver, and congenital heart disease.

Overall trends vs. individual trajectories in the second-to-fourth digit (2D:4D) and metacarpal (2M:4M) ratios during puberty and adolescence.

OBJECTIVES: The second-to-fourth digit ratio (2D:4D ratio) can be assumed a valid indicator of sexual differentiation, provided that it is stable once it develops, or eventual changes remain uniform in a respective cohort throughout ontogenesis. The main goal of this study was to determine whether the metacarpal 2M:4M and the digit 2D:4D ratio change during the period of pubertal/adolescent growth. MATERIALS AND METHODS: The metacarpals and digits were measured on radiographs of left hands in the sample of 328 individuals (96 pairs of male and 68 pairs of female twins) from the Wroclaw Longitudinal Study of Twins (1967-1983). Five consecutive annual measurements were done for each individual within a 4-year-interval somewhere between 7 and 18 years of age. Age-related changes in both ratios were studied using a set of mixed-effects linear models. Three types of correlation coefficients were used for assessment of stability between repeated measurements at different ages. RESULTS: An overall decrease in the average 2M:4M ratio was observed, attributable to a much larger extent to males than to females. On the contrary, a slight overall increase in the average 2D:4D ratio was observed, attributable to a much larger extent to females than to males. The rank order of the ratios remained highly stable within the monitored period (the correlation coefficient mostly ranged between 0.85 and 0.95). In spite of these findings, we recorded significant intraindividual changes in both ratios. In some individuals the 2D:4D ratio can undergo changes comparable to average sex differences and much higher than average age-related changes. DISCUSSION: Relatively slight overall changes in digit ratio in puberty and adolescence themselves are not inconsistent with the use of the 2D:4D ratio as an indirect marker of prenatal sexual differentiation. Nevertheless, individual changes in the ratios varied substantially in this study and differed from the average trends. Future studies should focus on the nature of interindividual developmental differences in the digit and metacarpal ratios.

Sex assessment using clavicle measurements: inter- and intra-population comparisons.

We studied sexual dimorphism of the human clavicle in order to describe size variation and create population-specific discriminant tools for morphometric sex assessment. The studied sample consisted of 200 skeletons of adult individuals obtained from the University of Athens Human Skeletal Reference Collection, Athens, Greece. The specimens were well-documented and represented a modern population from cemeteries in the Athens area. Six dimensions typically used for clavicle measurements were recorded. For sexing clavicles, we used both traditional univariate (limiting, demarking and sectioning points) and multivariate discriminant function analysis. The accuracy of the best five classification equations/functions ranged from 91.62% to 92.55% of correctly assigned specimens. By testing new and previously published sexing functions (Greeks, Polynesians, Guatemalans) on four available population samples (English, Indians from Amritsar, Indians from Varanasi, and data from the present study) we found that, for some combinations of tested and reference samples, the accuracy of the sex assessment may decrease even below the probability given by random sex assignment. Therefore, measurements of the clavicle should not be used for sex assessment of individual cases (both forensic and archeological) whose population origin is unknown. However, significant metric differences were also recorded among three different Greek samples (i.e. within a population). As a consequence, application of a sexing method generated from one Greek sample and applied to another Greek sample led to negligible reduction in the success of sex assessment, despite general similarities in ethnic origin (Greeks), generation structure and presumed social background of the samples. Therefore, we believe that future studies should focus on understanding the nature of the differences among within-population reference samples.

Investigation of interaction between magnetic silica particles and lambda phage DNA fragment.

Nucleic acids belong to the most important molecules and therefore the understanding of their properties, function and behavior is crucial. Even though a range of analytical and biochemical methods have been developed for this purpose, one common step is essential for all of them - isolation of the nucleic acid from the from complex sample matrix. The use of magnetic particles for the separation of nucleic acids has many advantages over other isolation methods. In this study, an isolation procedure for extraction of DNA was optimized. Each step of the isolation process including washing, immobilization and elution was optimized and therefore the efficiency was increased from 1.7% to 28.7% and the total time was shortened from 75 to 30min comparing to the previously described method. Quantification of the particular parameter influence was performed by square-wave voltammetry using hanging drop mercury electrode. Further, we compared the optimized method with standard chloroform extraction and applied on isolation of DNA from Staphylococcus aureus and Escherichia coli.