C. elegans chromosomes connect to centrosomes by anchoring into the spindle network.

The mitotic spindle ensures the faithful segregation of chromosomes. Here we combine the first large-scale serial electron tomography of whole mitotic spindles in early C. elegans embryos with live-cell imaging to reconstruct all microtubules in 3D and identify their plus- and minus-ends. We classify them as kinetochore (KMTs), spindle (SMTs) or astral microtubules (AMTs) according to their positions, and quantify distinct properties of each class. While our light microscopy and mutant studies show that microtubules are nucleated from the centrosomes, we find only a few KMTs directly connected to the centrosomes. Indeed, by quantitatively analysing several models of microtubule growth, we conclude that minus-ends of KMTs have selectively detached and depolymerized from the centrosome. In toto, our results show that the connection between centrosomes and chromosomes is mediated by an anchoring into the entire spindle network and that any direct connections through KMTs are few and likely very transient.

Anisotropic sampling of planar and two-manifold domains for texture generation and glyph distribution.

We present a new method for the generation of anisotropic sample distributions on planar and two-manifold domains. Most previous work that is concerned with aperiodic point distributions is designed for isotropically shaped samples. Methods focusing on anisotropic sample distributions are rare, and either they are restricted to planar domains, are highly sensitive to the choice of parameters, or they are computationally expensive. In this paper, we present a time-efficient approach for the generation of anisotropic sample distributions that only depends on intuitive design parameters for planar and two-manifold domains. We employ an anisotropic triangulation that serves as basis for the creation of an initial sample distribution as well as for a gravitational-centered relaxation. Furthermore, we present an approach for interactive rendering of anisotropic Voronoi cells as base element for texture generation. It represents a novel and flexible visualization approach to depict metric tensor fields that can be derived from general tensor fields as well as scalar or vector fields.