Ancient DNA.

DNA that has been recovered from archaeological and palaeontological remains makes it possible to go back in time and study the genetic relationships of extinct organisms to their contemporary relatives. This provides a new perspective on the evolution of organisms and DNA sequences. However, the field is fraught with technical pitfalls and needs stringent criteria to ensure the reliability of results, particularly when human remains are studied.

Molecular analyses of oral polio vaccine samples.

It has been suggested that the human immunodeficiency virus (HIV), and thus the acquired immunodeficiency syndrome (AIDS) it causes, was inadvertently introduced to humans by the use of an oral polio vaccine (OPV) during a vaccination campaign launched by the Wistar Institute, Philadelphia, PA, USA, in the Belgian Congo in 1958 and 1959. The "OPV/AIDS hypothesis" suggests that the OPV used in this campaign was produced in chimpanzee kidney epithelial cell cultures rather than in monkey kidney cell cultures, as stated by H. Koprowski and co-workers, who produced the OPV. If chimpanzee cells were indeed used, this would lend support to the OPV/AIDS hypothesis, since chimpanzees harbor a simian immunodeficiency virus, widely accepted to be the origin of HIV-1. We analyzed several early OPV pools and found no evidence for the presence of chimpanzee DNA; by contrast, monkey DNA is present.

A molecular analysis of dietary diversity for three archaic Native Americans.

DNA was extracted from three fecal samples, more than 2,000 years old, from Hinds Cave, Texas. Amplification of human mtDNA sequences showed their affiliation with contemporary Native Americans, while sequences from pronghorn antelope, bighorn sheep, and cottontail rabbit allowed these animals to be identified as part of the diet of these individuals. Furthermore, amplification of chloroplast DNA sequences identified eight different plants as dietary elements. These archaic humans consumed 2-4 different animal species and 4-8 different plant species during a short time period. The success rate for retrieval of DNA from paleofeces is in strong contrast to that from skeletal remains where the success rate is generally low. Thus, human paleofecal remains represent a source of ancient DNA that significantly complements and may in some cases be superior to that from skeletal tissue.

A pharmacokinetic-pharmacodynamic modelling of the antihistaminic (H1) effects of cetirizine.

AIM: The pharmacokinetic-pharmacodynamic modelling developed here characterizes the time course of cetirizine effect on histamine-induced skin reactions (wheal and flare). METHOD: The model incorporated data from the study of Simons et al. [1993] in which the cetirizine plasma concentrations and the wheal and flare areas were recorded in a group of 6 patients after a 10 mg oral administration. RESULTS: The peak plasma concentration (>500 ng/ml) was rapidly reached in 1 h and the maximal effects were observed later at approximately 6 h. The cetirizine effect was ascribed to a physiologic indirect response model in which the drug concentration in the central compartment is linked to a response function that describes the inhibition or stimulation of the factors affected, input or output of response control. Cetirizine was characterized by two-compartmental kinetics with a rapid absorption phase (Ka = 1.0-1.4 h(-1)), a rapid distribution phase (alpha = 0.33-0.69 h(-1)) and a slower terminal half-life, 13.2-13.6 h (beta = 0.051-0.052 h(-1)). The total clearance was 1.4-1.5 l/h. Cetirizine effects on flare and wheal were characterized by the inhibition of the input factor (k(in)), the concentrations producing 50% of maximal effect (EC50) were 13 and 40 ng/ml and k(in) were 0.99 and 0.96 h(-1), respectively. These results were then used to simulate repeated daily oral administration of 10 mg cetirizine. CONCLUSION: At this dosage the histamine-induced flare was at least 80% inhibited at the start of the second administration Thereafter, on successive administrations, the inhibition was even more pronounced and the response control was nearly total.

[Efficacy of mononitrate retard therapy in patients with advanced congestive heart failure]. / Skutecznosc monoazotanu retard u chorych z zaawansowana niewydolnoscia krazenia.

To determine the efficacy of mononitrate retard therapy in congestive heart failure 54 pts (42 males and 12 females, aged 67.2 +/- 8.7 yrs.) with NYHA functional class 1-3 and left ventricular ejection fraction less than 40% were investigated. Clinical examination, exercise treadmill test (ETT), ecg holter monitoring and echocardiography (echo-2D) were performed before and after 4 weeks of therapy with Olicard 40 mg Retard. 4 weeks treatment with mononitrate improved clinical parameters. The shift to lower functional NYHA class was observed in 12 cases (p < 0.01). Number of anginal pains per week was reduced from average 3.15 to 1.55 (p < 0.01). Mononitrate therapy improved exercise tolerance during ETT. Exercise time increased from 424 +/- 168 to 568 +/- 143 sec. (p < 0.001) as well as total workload in METS (3.6 +/- 1.4 vs. 4.9 +/- 1.9, p < 0.001). The time to 0.1 mV ischemic ST segment depression was extended from 215 +/- 149 to 357 +/- 173 sec. (p < 0.01). Holter monitoring revealed moderate increase in heart rate and significant reduction of ventricular arrhythmia (p < 0.05). No changes in systolic and diastolic echo-2D parameters were observed.

[Life threatening salicylate poisoning caused by percutaneous absorption in severe ichthyosis vulgaris]. / Lebensbedrohliche Salicylatintoxikation durch perkutane Resorption bei einer schweren Ichthyosis vulgaris.

In a 7-year-old boy, ichthyosis vulgaris was treated with a 10% ointment for application over a large area of the body surface. In this way, the child received 400 g salicylic acid (0.6 g/kg body weight per day) percutaneously over a period of 4 weeks. The patient was referred to hospital by the family doctor: he was in a deep somnolent state, apparently caused by hyperventilation following wheezing, vomiting, tinnitus and vertigo. Salicylate intoxication was suspected because of metabolic acidosis, an anion gap and respiratory overcompensation. The diagnosis was confirmed by a serum salicylate level of 985 micrograms/ml (therapeutic level 150-300 micrograms/ml). Following forced diuresis and alkalization with sodium bicarbonate, haemodialysis was unnecessary. As the salicylate level declined to values within the therapeutic range, the patient started to recover consciousness, waking on the 4th day. By day 6 there were still obvious neurological deficiencies. Fecal incontinence, bilateral ptosis and intermittent diverging strabismus on the right persisted for some weeks. It was 6 months before complete neurological resolution was achieved. The pathogenesis of salicylate toxicity and the need for safer therapies for ichthyosis vulgaris are discussed.

[Multi-plane transesophageal echocardiography--the next step to 3 dimensional imaging of the heart]. / Wieloplaszczyznowa echokardiografia przezprzelykowa--kolejny krok do trojwymiarowej oceny serca.

The multiplane transesophageal echocardiography (MTEE) is a new method making the precise imaging of the heart and great vessels possible. That technique allows to obtain the images of cardiovascular structures in two perpendicular investigation planes ("biplane" operation): transverse (examination angle is equal to 90 degrees--in relation to long axis of esophagus) and longitudinal = sagittal (examination angle is equal to 0 degree--in relation to long axis of esophagus) and, as well, in all medial planes enclosed from 0 degree to 90 degrees examination angle. During the investigation planes were being rotated mechanically or manually by rotation wheel. Passing from one plane to another is fluent, due to that the heart and main vessels can be observed continuously. Sector angle variability is 180 degrees (from minus 90 degrees to plus 90 degrees). The investigation planes from 0 degrees to plus 90 degrees from so called "normal" visualization field, and planes from 90 degrees to 0 degree make a "mirror" reflection of the "normal" field (mirror field). In our investigations the Vingmed-Sonotron echocardiograph connected with 5 MHz annular phased--array transducer, placed at the end of flexible endoscope was used. The multiplanar transducers have a full Doppler option (pulsed and continuous waves) with the possibility of colour flow imaging. The initial experience with the new method is presented in this report. The MTEE enables precise assessment of atrial septal defects. The multiplane imaging allows complete evaluation of heart muscle thickness and left ventricular contractility. This technique gives a better visualization of coronary artery. MTEE permits for planimetric assessment of aortic valve.(ABSTRACT TRUNCATED AT 250 WORDS)

[Two-dimensional transesophageal echocardiography (clinical value)]. / Dwuplaszczyznowa echokardiografia przezprzelykowa (wartosc kliniczna).

The introduction of two-dimensional echocardiography (echo 2D) complemented by doppler techniques has allowed to assess the dynamic function of the heart. However in 10-15% patients the standard transthoracic method (TEE) does not provide complete echocardiographic image due to obesity, emphysema and deformations of thorax. These difficulties have been later overcome by transesophageal probe, but it made the examination possible only in one plane-monoplane TEE (m-TEE). Transesophageal echocardiography the biplane probe (bi-TEE), introduced in late 80s has permitted the heart and aorta visualization in two perpendicular planes: transverse (T) and longitudinal (L). The purpose of our study was to establish the diagnostic value of biplane transesophageal echocardiography in comparison with hitherto existing monoplane echocardiography (transverse plane). The study group consists of 60 patients (aged 19-78 years) with various diseases of heart and aorta. We performed biplane transesophageal examinations with the use of Aloka SSD-870 echocardiograph connected with the biplane probe (45 patients) or new, prototypical matrix probe (15 patients). For the heart and aorta assessment the typical projections were used. The advantages of biplane TEE compared with monoplane TEE are as follows: 1) more favorable left ventricular examination, 2) better assessment of the heart apex, 3) the ability to investigate the right heart; tricuspid valve, right ventricular outflow tract, pulmonary valve, pulmonary trunk and right pulmonary artery, 4) precise imaging of both atrial' structures: cavities, intraatrial septum, foramen ovale, left atrial appendage, venae cavae and pulmonary veins, 5) possibility of thoracic aorta diagnostics, especially in ascending aorta.(ABSTRACT TRUNCATED AT 250 WORDS)

[Transesophageal and classical echocardiography in myocardial infarction and heart valve disease (comparison study)]. / Echokardiografia przezprzelykowa i klasyczna w zawale serca i wadach zastawkowych (badanie porównawcze).

In 85 patients (22 with myocardial infarct and 63 with valvular heart disease) echocardiographical examination simultaneously by classical and transesophageal methods was done. The transesophageal method was better in assessing the aortic valve and similar to the classical method in reference to the mitral valve. Heart contractility assessment was better with the classical method. Transesophageal echocardiography is the method of choice in patients in whom classical echocardiography cannot be done (obesity, emphysema, chest deformation).

[Transesophageal echocardiography--a method of evaluation of the aortic and mitral valves]. / Echokardiografia przezprzelykowa--metoda oceny zastawek: aortalnej i mitralnej.

Transoesophageal echocardiography (TEE) is a new diagnostic method introduced for clinical practice about 1985. The method is particularly useful for the evaluation of the aorta and mitral valves. Twenty-seven patients with pathological changes of the valves of the left side of the heart were studied. In all patients the result of transthoracic echocardiography--TTE was non-diagnostic due to obesity, emphysema, deformity of the chest. In TEE diagnostic findings in the structures of the chest were obtained, their morphology and function were assessed, the clinical diagnosis was confirmed or verified. TEE was found to be useful in the evaluation of the valves, making possible establishing of correct diagnosis, especially in cases in which TTE was insufficient for providing of adequate information.

[Transesophageal echocardiography--our preliminary experience]. / Echokardiografia przezprzelykowa--wstepne doswiadczenia wlasne.

This study presents the initial experience of our department with transesophageal echocardiography. Transesophageal echocardiography (TEE) and transthoracic echocardiography (TTE) were performed simultaneously in 31 clinical patients (16 to 76 years old). 13 pts with aortic (9) and mitral (4) valve disease, 10 pts with ischemic heart disease (including 7 AMI), 3 pts with congestive cardiomyopathy, 2 pts with aortic aneurysm and 3 pts with other heart diseases. The investigations were performed with use of Aloka SSD 650 machine and 7.5 MHz transesophageal convex transducer. The patients were prepared for the examination in the typical way by local anesthesia, administration of sedatives and heart monitoring. The following projections were used: basic short- and long-axis view, four chamber view, and transgastric view. These investigation confirmed the usefulness TEE for evaluation of aortic and mitral valves, especially their morphology, atrial septum and atrial cavities. Contractility and other heart structures were difficult to be evaluated by TEE. It seems to be possible to estimate the proximal sections of the coronary arteries. Only few complications were found: supraventricular (2) and ventricular extrasystoles (8), transient supraventricular tachycardia (3), and bradycardia (3). Late complications were not found.

[Hypertension and obesity]. / Nadcisnienie tetnicze a otylosc.

An epidemiological study was carried out in a population of men aged 35-64 years in the Bródno District of Warsaw. The study included 2123 subjects, the frequency was noted of hypertension and obesity as risk factors for ischaemic heart disease, and their correlations were studied. As hypertension the following values were accepted: --borderline hypertension = 140/90 less than 160/95 mm Hg, --stabilized hypertension = greater than or equal to 160/95 mm Hg. Overweight was accepted as Quetelet's index 29.0 weight in kg/height in cm. Stable hypertension was found in 35.0% of obese men and 18.5% of non-obese men (p less than 0.001). Borderline hypertension was demonstrated in 31.6% of obese men and 22.9% of non-obese men (p less than 0.002). Normal blood pressure was found in 58.4% of non-obese men and in only 33.4% of the obese ones (p less than 0.001). The prevalence of hypertension in obese men, both systolic and diastolic hypertension, was related to age: the highest proportion of hypertensive subjects was in the oldest group, the lowest one in the youngest group. The results agree with those reported by other authors who also demonstrated a high correlation between hypertension occurrence and increasing overweight.