ABSTRACT
Steroid hormones are often used in animal agriculture but are currently banned for use in domesticated fowl because residual hormones could be present in eggs for human consumption. Egg samples from eight common commercial poultry layer breeds (Hy-Line W-36, Hy-Line Brown, ISA-White, ISA-Brown, Lohnmann Ultra-Lite, Lohnmann-Brown, Hisex White, Hisex Brown) in Taiwan were screened for a combination of 15 natural and synthetic steroid hormones by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for consumer assurance. Only natural hormones such as progesterone, 4-androstene-3,17-dione, and testosterone were detected. Regarding each breed, the interaction effect (age × shell color), main effect (age or shell color), and blocking effect (lighting system) were further analyzed by using 2 × 2 factorial arrangement of treatment in a randomized block design. We also discovered associations between yolk steroid hormone levels and laying hen age, as well as lighting conditions. Additionally, we found a correlation between hormone levels and eggshell color, suggesting a potential role in brown pigmentation. Ultimately, we concluded that detectable steroid hormone levels in eggs were not a consumer health risk. Furthermore, these data provide empirical hormone concentrations in various types of commercial layer breeds for future research.
ABSTRACT
4-[2-(t-Butylamino)-1-hydroxyethyl]phenol (buctopamine, 4), a new ß2 receptor agonist (ß2-agonist), was found to be an adulterant in feed additives for swine in Taiwan, where using ß2-agonists in food-production animals is prohibited. Buctopamine and its metabolite, 4-[2-(t-butylamino)-1-hydroxyethyl]-2-methoxyphenol (mebuctopamine, 2), were detected in swine hair specimens. Authentic compounds 2 and 4 were synthesized with 98.6% and 97.7% purity, respectively, as reference standards for analysis, and both compounds were more hydrophilic than ractopamine and clenbuterol. In a preliminary pharmacological evaluation, compounds 2 and 4 exhibited moderate human ß2 receptor binding affinity and did not show significant affinities for the human α1, α2, ß1, and ß3 receptors. After addition of compounds 2-4 into the ß2-agonist library, a multiresidue analysis of 26 ß2-agonists by using triple quadrupole LC/MS/MS for routine screening conducted by regulatory authorities was established, in which the common limits of quantification for the 26 ß2-agonists in swine feed and hair are 10 and 25 ng/g, respectively. In addition, the illegal use of buctopamine (4) has been effectively prevented. The results of this study are also useful for controlling the illegal use of new ß2-agonists in food-production animals.
