Genetic ablation of Immt induces a lethal disruption of the MICOS complex.

The mitochondrial contact site and cristae organizing system (MICOS) is important for crista junction formation and for maintaining inner mitochondrial membrane architecture. A key component of the MICOS complex is MIC60, which has been well studied in yeast and cell culture models. However, only one recent study has demonstrated the embryonic lethality of losing Immt (the gene encoding MIC60) expression. Tamoxifen-inducible ROSA-CreERT2-mediated deletion of Immt in adult mice disrupted the MICOS complex, increased mitochondria size, altered cristae morphology, and was lethal within 12 d. Pathologically, these mice displayed defective intestinal muscle function (paralytic ileus) culminating in dehydration. We also identified bone marrow (BM) hypocellularity in Immt-deleted mice, although BM transplants from wild-type mice did not improve survival. Altogether, this inducible mouse model demonstrates the importance of MIC60 in vivo, in both hematopoietic and non-hematopoietic tissues, and provides a valuable resource for future mechanistic investigations into the MICOS complex.

A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction.

Contact sites between lipid droplets and other organelles are essential for cellular lipid and energy homeostasis. Detection of these contact sites at nanometer scale over time in living cells is challenging. Here, we developed a tool kit for detecting contact sites based on Fluorogen-Activated Bimolecular complementation at CONtact sites, FABCON, using a reversible, low affinity split fluorescent protein, splitFAST. FABCON labels contact sites with minimal perturbation to organelle interaction. Via FABCON, we quantitatively demonstrated that endoplasmic reticulum (ER)- and mitochondria (mito)-lipid droplet contact sites are dynamic foci in distinct metabolic conditions, such as during lipid droplet biogenesis and consumption. An automated analysis pipeline further classified individual contact sites into distinct subgroups based on size, likely reflecting differential regulation and function. Moreover, FABCON is generalizable to visualize a repertoire of organelle contact sites including ER-mito. Altogether, FABCON reveals insights into the dynamic regulation of lipid droplet-organelle contact sites and generates new hypotheses for further mechanistical interrogation during metabolic switch.

ICESat-2 Early Mission Synopsis and Observatory Performance.

The Advanced Topographic Laser Altimetry System (ATLAS) onboard the NASA Ice, Cloud, and land Elevation Satellite-2 (ICESat-2) is the newest Earth observing satellite for global elevation studies. The primary objectives for ICESat-2 follow the objectives of its predecessor, ICESat and also focus on providing cryospheric measurements to determine ice sheet mass balance, and monitor both sea ice thickness and extent. However, the global observations support secondary science objectives as well such as biomass estimation, inland water elevation, sea state height and aerosol concentrations. Since launch of ICESat-2, ATLAS has collected more than a trillion measurements. This study provides a mission overview, a description of the operational components that enable the altimeter products for science, on-orbit observatory performance, and assessment of the spacecraft attitude control systems that enable repeat measurements to within 10 m and pointing control within ±45 m. These metrics should be considered for ground-based validation campaigns or science investigations.

The Ice, Cloud, and Land Elevation Satellite - 2 Mission: A Global Geolocated Photon Product Derived From the Advanced Topographic Laser Altimeter System.

The Ice, Cloud, and land Elevation Satellite - 2 (ICESat-2) observatory was launched on 15 September 2018 to measure ice sheet and glacier elevation change, sea ice freeboard, and enable the determination of the heights of Earth's forests. ICESat-2's laser altimeter, the Advanced Topographic Laser Altimeter System (ATLAS) uses green (532 nm) laser light and single-photon sensitive detection to measure time of flight and subsequently surface height along each of its six beams. In this paper, we describe the major components of ATLAS, including the transmitter, the receiver and the components of the timing system. We present the major components of the ICESat-2 observatory, including the Global Positioning System, star trackers and inertial measurement unit. The ICESat-2 Level 1B data product (ATL02) provides the precise photon round-trip time of flight, among other data. The ICESat-2 Level 2A data product (ATL03) combines the photon times of flight with the observatory position and attitude to determine the geodetic location (i.e. the latitude, longitude and height) of the ground bounce point of photons detected by ATLAS. The ATL03 data product is used by higher-level (Level 3A) surface-specific data products to determine glacier and ice sheet height, sea ice freeboard, vegetation canopy height, ocean surface topography, and inland water body height.

Estimation of sea-ice thickness and volume in the Sea of Okhotsk based on ICESat data.

Sea-ice thickness in the Sea of Okhotsk is estimated for 2004-2008 from ICESat derived freeboard under the assumption of hydrostatic balance. Total ice thickness including snow depth (h tot ) averaged over 2004-2008 is 95 cm. The interannual variability of h tot is large; from 77.5 cm (2008) to 110.4 cm (2005). The mode of h tot varies from 50-60 cm (2007 and 2008) to 70-80 cm (2005). Ice thickness derived from ICESat data is validated from a comparison with that observed by Electromagnetic Induction Instrument (EM) aboard the icebreaker Soya near Hokkaido, Japan. Annual maps of h tot reveal that the spatial distribution of h tot is similar every year. Ice volume of 6.3 × 1011 m3 is estimated from the ICESat derived h tot and AMSR-E derived ice concentration. A comparison with ice area demonstrates that the ice volume cannot always be represented by the area solely, despite the fact that the area has been used as a proxy of the volume in the Sea of Okhotsk. The ice volume roughly corresponds to that of annual ice production in the major coastal polynyas estimated based on heat budget calculations. This also supports the validity of the estimation of sea-ice thickness and volume using ICESat data.

Characterizing Arctic sea ice topography using high-resolution IceBridge data.

We present an analysis of Arctic sea ice topography using high resolution, three-dimensional, surface elevation data from the Airborne Topographic Mapper, flown as part of NASA's Operation IceBridge mission. Surface features in the sea ice cover are detected using a newly developed surface feature picking algorithm. We derive information regarding the height, volume and geometry of surface features from 2009-2014 within the Beaufort/Chukchi and Central Arctic regions. The results are delineated by ice type to estimate the topographic variability across first-year and multi-year ice regimes. The results demonstrate that Arctic sea ice topography exhibits significant spatial variability, mainly driven by the increased surface feature height and volume (per unit area) of the multi-year ice that dominates the Central Arctic region. The multi-year ice topography exhibits greater interannual variability compared to the first-year ice regimes, which dominates the total ice topography variability across both regions. The ice topography also shows a clear coastal dependency, with the feature height and volume increasing as a function of proximity to the nearest coastline, especially north of Greenland and the Canadian Archipelago. A strong correlation between ice topography and ice thickness (from the IceBridge sea ice product) is found, using a square-root relationship. The results allude to the importance of ice deformation variability in the total sea ice mass balance, and provide crucial information regarding the tail of the ice thickness distribution across the western Arctic. Future research priorities associated with this new dataset are presented and discussed, especially in relation to calculations of atmospheric form drag.