ABSTRACT
AIM: to evaluate changes in visual acuity and compliance after CAM therapy in patients that initially failed to respond to occlusion or were non-compliant. MATERIAL AND METHODS: Twenty nine patients aged 4-10 years that failed to improve under occlusion therapy were included in this prospective study. Patients were divided into two groups according to the age (16 patients of 4-7 years of age and 13 patients of 8-10 years of age) and two other groups according to the type of amblyopia (15 anisometropic and 14 strabismic cases). CAM stimulation was prescribed to all patients for six days. Occlusion therapy was then recommenced. Best corrected visual acuity was measured before the CAM course, immediately after, at 3- and 6-month follow-ups, and on the last visit. RESULTS: Visual acuities in all study groups increased significantly immediately after the CAM therapy with further improvement during the follow-up period. CONCLUSION: CAM therapy for amblyopia is helpful in improving visual acuity as well as patients' compliance to subsequent occlusion therapy.
Subject(s)
Amblyopia , Photic Stimulation/methods , Sensory Deprivation/physiology , Amblyopia/diagnosis , Amblyopia/physiopathology , Amblyopia/psychology , Amblyopia/therapy , Anisometropia/physiopathology , Child , Child, Preschool , Female , Humans , Male , Patient Compliance , Retrospective Studies , Strabismus/physiopathology , Treatment Outcome , Turkey , Visual AcuityABSTRACT
The plant chemistry and cytotoxic activity of Colchicum baytopiorum CD Brickell (Liliaceae/Colchicaceae), an endemic species growing in Turkey, has been studied for the first time. Nine known alkaloids were isolated and their structures were identified by spectral methods (UV, IR, (1)H-NMR, and ESI/MS), and the presence of three alkaloids, which could not be isolated from the plant, was also detected by LC/MS/MS spectrometry. Phenolic acids were elaborated using LC/MS and 11 phenolic acids were identified. The presence of two flavonoids appeared to be valuable for chemotaxonomic purposes. Guided by the brine shrimp lethality test (BSLT), methanol extracts were tested for cytotoxic activity by colorimetric MTT test on K562 and HL60 cells. Except the seed extract, all methanol extracts showed more cytotoxic activity on HL60 cells (IC(50): 6.5- < 0.1 microg/mL) than on K562 cells (IC(50): > 500-44 microg/mL).