ABSTRACT
Males and females in sexually dimorphic species show differences in their physiology and behaviour due to differences in energetic investment into reproduction and soma. This means that the two sexes may show different patterns of parasitism at different times of the year. In this study, we evaluate the abundance of fecal eggs and larvae of 5 parasite types (Strongyles, Nematodirus spp., Marshallagia marshalli., Protostrongylus spp. lungworms, and Eimeria spp.) in relation to season and sex in Rocky Mountain bighorn sheep (Ovis canadensis). We use fecal egg counts (FEC) as a proxy for infection intensity. Parasite FECs differed between male and female bighorn sheep and varied with season. We found pronounced fluctuations in fecal egg counts of various parasite species in males and females across different seasons and reproductive stages. Strongyle counts were significantly higher during late gestation and lactation/summer, and particularly pronounced in males. Nematodirus counts were highest during late gestation in females and during the rut in males. Marshallagia counts peaked during late gestation in females and during the rut in males. Protostrongylus spp. lungworm counts were highest during late gestation in females and in males during lactation/summer and the rut. Eimeria oocyst counts varied across seasons, with higher counts in males during the rut and in females during winter and late gestation. Additionally, significant differences in Strongyle counts were observed between coursing and tending rams, with tending rams exhibiting higher counts. We discuss why the sexes might differ in FECs and suggest that differences between FECs of the parasites across seasons may be due to different life cycles and cold tolerance of the parasites themselves.
ABSTRACT
BACKGROUND: Muskoxen are important ecosystem components and provide food, economic opportunities, and cultural well-being for Indigenous communities in the Canadian Arctic. Between 2010 and 2021, Erysipelothrix rhusiopathiae was isolated from carcasses of muskoxen, caribou, a seal, and an Arctic fox during multiple large scale mortality events in the Canadian Arctic Archipelago. A single strain ('Arctic clone') of E. rhusiopathiae was associated with the mortalities on Banks, Victoria and Prince Patrick Islands, Northwest Territories and Nunavut, Canada (2010-2017). The objectives of this study were to (i) characterize the genomes of E. rhusiopathiae isolates obtained from more recent muskox mortalities in the Canadian Arctic in 2019 and 2021; (ii) identify and compare common virulence traits associated with the core genome and mobile genetic elements (i.e. pathogenicity islands and prophages) among Arctic clone versus other E. rhusiopathiae genomes; and iii) use pan-genome wide association studies (GWAS) to determine unique genetic contents of the Arctic clone that may encode virulence traits and that could be used for diagnostic purposes. RESULTS: Phylogenetic analyses revealed that the newly sequenced E. rhusiopathiae isolates from Ellesmere Island, Nunavut (2021) also belong to the Arctic clone. Of 17 virulence genes analysed among 28 Arctic clone isolates, four genes - adhesin, rhusiopathiae surface protein-A (rspA), choline binding protein-B (cbpB) and CDP-glycerol glycerophosphotransferase (tagF) - had amino acid sequence variants unique to this clone when compared to 31 other E. rhusiopathiae genomes. These genes encode proteins that facilitate E. rhusiopathiae to attach to the host endothelial cells and form biofilms. GWAS analyses using Scoary found several unique genes to be overrepresented in the Arctic clone. CONCLUSIONS: The Arctic clone of E. rhusiopathiae was associated with multiple muskox mortalities spanning over a decade and multiple Arctic islands with distances over 1000 km, highlighting the extent of its spatiotemporal spread. This clone possesses unique gene content, as well as amino acid variants in multiple virulence genes that are distinct from the other closely related E. rhusiopathiae isolates. This study establishes an essential foundation on which to investigate whether these differences are correlated with the apparent virulence of this specific clone through in vitro and in vivo studies.
Subject(s)
Erysipelothrix , Arctic Regions , Erysipelothrix/genetics , Erysipelothrix/pathogenicity , Erysipelothrix/isolation & purification , Canada , Animals , Virulence/genetics , Genomics , Genome, Bacterial , Phylogeny , Erysipelothrix Infections/microbiology , Virulence Factors/genetics , Genome-Wide Association Study , Genomic IslandsABSTRACT
Parasites negatively affect the fitness of ungulate hosts directly, and in wild ungulates, these effects may be synzootic with other stressors, such as limited nutritional resources. In the Arctic, muskoxen (Ovibos moschatus) occur in a highly seasonal environment and must rely on finite energetic resources for survival and productivity. We investigated the costs of gastrointestinal nematodes on the body condition and reproductive status of 141 muskoxen, on Banks Island, Canada, when the population was at a peak in numbers and density. Using a Partial Least Squares Path Modelling approach, we found that high adult nematode abundance was associated with lower body condition, and high parasite abundance was associated with female reproduction including the indirect effect through on body condition (n = 87). These findings suggest that individuals prioritize energetic reserves for reproduction over parasite defence. In fall 2003, a severe icing event that restricted access to forage was associated with high overwinter mortality of muskoxen and a population crash. Through direct and indirect costs of parasite infection on body condition and reproduction, the high abundance of parasites may have contributed to the effects of this extreme weather event. Understanding the mechanisms in which parasites impact fitness can help explain the ecological drivers of ungulate populations and predict the interactions between the environment and populations.
Subject(s)
Ruminants , Animals , Arctic Regions , Ruminants/parasitology , Female , Host-Parasite Interactions , Reproduction , Population Dynamics , Seasons , Nematoda/physiology , Nematoda/pathogenicity , Male , Canada , Nematode Infections/veterinary , Nematode Infections/parasitologyABSTRACT
Age estimation is crucial for investigating animal populations in the past and present. Visual examination of tooth wear and eruption is one of the most common ageing methods in zooarchaeology, wildlife management, palaeontology, and veterinary research. Such approaches are particularly advantageous because they are non-destructive, can be completed using photographs, and do not require specialized training. Several tooth wear and eruption methods have been developed for Rangifer tarandus, a widely distributed and long-utilized species in the North. This paper evaluates the practicality and effectiveness of three existing visual tooth wear and eruption methods for this species using a large known-age sample from several caribou populations in northern Canada (Bluenose East, Bluenose West, Dolphin-Union, Qamanirjuaq, and Beverly herds). These methods are evaluated based on: (1) the amount of error and bias between estimated and actual ages, (2) suitable and interpretable results, (3) user-friendly and unambiguous procedures, and (4) which teeth and visual features of those teeth are used to record wear and eruption status. This study finds that the three evaluated methods all have variable errors and biases, and two show extensive biases when applied to older individuals. Demographic data is simpler to generate and more flexible to report when methods allow age to be estimated as a continuous or discrete variable, rather than as age ranges. The dentition samples used by two of the previously developed methods impact their applicability to other populations of Rangifer. In one existing method, individuals were unavailable from some age ranges leaving gaps when assigning ages. For another Rangifer-ageing method, the population utilized was too distinct in morphology or diet to be used with the Canadian caribou analyzed here. Additional refinement of tooth wear and eruption ageing methods will benefit zooarchaeological research on reindeer and caribou remains.
Subject(s)
Deer , Reindeer , Animals , Canada , Animals, Wild , DietABSTRACT
Brucellosis is an important zoonotic disease affecting animals and subsistence harvesters in the circumarctic. We investigated recent trends (2015-2022) of brucellosis seropositivity in caribou (Rangifer tarandus) and muskoxen (Ovibos moschatus) in the Central Canadian Arctic by using data from community-based wildlife health surveillance programs. The overall sample prevalence of Brucella antibodies was 10.0% (n = 271) in muskoxen and 15.5% (n = 277) in caribou. Sample seroprevalence in muskoxen varied geographically with an increasing trend of exposure on NW Victoria Island (from 0% to 36.8% between 2016 and 2022; Kendall tau = 0.283, p = 0.001). The presence of Brucella suis biovar 4 was confirmed by culture from clinical cases in this area. Our results indicate that Brucella suis biovar 4 continues to circulate in the Central Canadian Arctic in caribou and muskoxen and may be now circulating in muskoxen independently from caribou. These findings highlight the need to better understand the ecology and drivers of brucellosis emergence in Arctic multi-host systems.
ABSTRACT
Treponema spp. are associated with infectious lameness in livestock and wild ruminants. While extensive research has been conducted on cattle, investigations in wild ruminants are scarce. Hoof disease is common in caribou populations (Rangifer tarandus), but investigations are limited due to the remoteness of the Arctic. Our study aimed to assess the presence of Treponema spp. associated with bovine digital dermatitis in caribou. DNA was extracted from coronary band tissues from forty-eight caribou without visible hoof lesions and analyzed using two PCR methods (qPCR and nPCR). Treponema spp. were detected in low copy numbers/mg of tissue (3.6 to 6.6 × 101). T. phagedenis was the most prevalent and abundant species in 58% of samples by qPCR, followed by T. medium (44%), and T. pedis (10%). The qPCR and nPCR agreement ranged between 65% and 75% (Cohen's kappa 0.22-0.51). Sanger sequencing of thirteen nPCR products confirmed that treponemes in caribou are remarkably similar to those found in domestic ruminants and wild elk. Our study highlights the colonization of treponemes in healthy hooves of a wild ruminant in the Arctic, where there is no presence of livestock, and expands knowledge on the host range and distribution of treponemes. These findings also emphasize the need for further research into the multifactorial nature of treponema-associated hoof diseases and the putative role of treponemes in infectious lameness affecting caribou.
Subject(s)
Cattle Diseases , Digital Dermatitis , Reindeer , Treponemal Infections , Cattle , Animals , Treponema/genetics , Lameness, Animal , Animals, Wild , Treponemal Infections/veterinaryABSTRACT
Segmental analyses of hair may be useful for measuring biomarkers over several seasons to years from a single sample. To attribute hair segments to specific time periods, a known chronological marker, or a hair growth rate, is needed. We examined guard hair growth rates of captive muskoxen (Ovibos moschatus) in Fairbanks, Alaska, USA. We sought to determine if a general growth rate could be applied across muskox populations, thus facilitating the use of segmental analyses for various biomarkers. We used archived samples from 16 muskoxen that had guard hairs sampled at six, 14, and 30 wk after shaving. We measured the lengths of 10 guard hairs per sample, calculated weekly and annual growth rates, and then fitted linear mixed-effects models to assess the effect of different covariates on hair growth rate. The period in which hair had been grown had a significant effect (P<0.05) on growth rate. Extrapolated annual hair growth rates were 277±40 mm/yr (weeks 0-6), 248±47 mm/yr (weeks 7-14), and 165±36 mm/yr (weeks 15-30), with an overall average rate of 210±14 mm/yr. These rates were significantly faster than those of free-ranging Greenland muskoxen-78 mm/yr as measured by stable isotope analyses-and varied intra-annually. This suggests that a universal growth rate cannot be generalized across muskox populations and time.
Subject(s)
Hair , Ruminants , Animals , Alaska , Greenland , SeasonsABSTRACT
Orf virus (genus Parapoxvirus) has been associated with gross skin lesions on muskoxen (Ovibos moschatus) from Victoria Island, Nunavut, Canada, where muskox populations are experiencing population declines. Orf virus causes painful proliferative and necrotizing dermatitis upon viral replication and shedding, which may lead to animal morbidity or mortality through secondary infections and starvation. Herpesvirus, known to cause gross lesions on skin and mucosa during active viral replication, has also been documented in muskoxen but to date has not been associated with clinical disease. Our objective was to characterize the variation of orf virus and herpesvirus in wild muskoxen of the Canadian Arctic. Tissue samples including gross skin lesions from the nose, lips, and/or legs were opportunistically collected from muskoxen on Victoria Island, Nunavut and Northwest Territories, and mainland Nunavut, Canada, from 2015 to 2017. Sampled muskoxen varied in age, sex, location, hunt type, and body condition. Tissues from 60 muskoxen were tested for genetic evidence of orf virus and herpesvirus infection using PCR targeting key viral genes. Tissues from 38 muskoxen, including 15 with gross lesions, were also examined for histological evidence of orf virus and herpesvirus infection. Eleven muskoxen (10 from Victoria Island and one from mainland Nunavut) with gross lesions had microscopic lesions consistent with orf virus infection. Muskox rhadinovirus 1, a gammaherpesvirus endemic to muskoxen, was detected in 33 (55%) muskoxen including 17 with gross lesions. In all tissues examined, there was no histological evidence of herpesvirus-specific disease. Sequencing and characterization of amplified PCR products using phylogenetic analysis indicated that a strain of orf virus, which appears to be unique, is likely to be endemic in muskoxen from Victoria Island and mainland Nunavut. Many of the muskoxen are also subclinically infected with a known muskox-endemic strain of herpesvirus.
Subject(s)
Herpesviridae Infections , Orf virus , Rhadinovirus , Animals , Canada/epidemiology , Orf virus/genetics , Phylogeny , Ruminants , Herpesviridae Infections/veterinaryABSTRACT
Objective: Subsidized dog care and population management programs (DPM) are often implemented for dog population control where for-profit veterinary care is inaccessible. However, impacts of such programs are rarely assessed. The goal of this project was to determine if and how previously collected intake data from ongoing high-volume spay-neuter clinics could be used to measure impacts of such DPM programs. Animals: We used intake data collected from 2008 to 2019 from spay-neuter clinics that had been delivered repeatedly over a 10-year period in 6 First Nations communities in Alberta, to assess changes in intake dog characteristics. Procedures: Numbers of dogs brought in for spay-neuter surgery or surrendered, and their ages, sexes, breeds, weights, and body condition scores were compared. Reasons for surrender were investigated and socioeconomic factors were investigated as possible drivers for community differences in clinic participation rates and clinic sex ratios. Results: Socioeconomic indicators did not differ between the 6 participating communities; however, the total number of clinics requested by communities varied. In early years, female dogs were more likely to be brought to the clinics to be spayed whereas, in later years, more males were seen. The age at which animals were brought in for spay or neuter decreased over time (P < 0.05) but the surrender age did not. Breed proportions of x-large, small, and x-small breeds varied over time for both spay-neuter and surrender dogs (P < 0.05), with large-breed dogs always being the most common. However, there was an increase in small and x-small breeds in the final years of the study. Finally, weight status, a calculated estimate of body condition of spay-neuter dogs, increased over time within large and medium breeds, whereas the body condition score of surrendered dogs shifted from "thin" to "ideal" over time. Conclusions and clinical relevance: This work highlights the potential and challenges of using intake data to assess impacts of spay-neuter clinics that occur repeatedly over many years in the same communities. Overall increased weight status, high participation rates, and reduction of age at intake suggest positive effects of the assessed spay-neuter programs.
Les données d'admission peuvent-elles renseigner sur les impacts des cliniques de stérilisation sur place pour les chiens répétées et subventionnées? Objectif: Des programmes subventionnés de soins canins et de gestion de la population (DPM) sont souvent mis en Åuvre pour limiter la population canine là où les soins vétérinaires à but lucratif sont inaccessibles. Cependant, les impacts de ces programmes sont rarement évalués. L'objectif de ce projet était de déterminer si et comment les données d'admission précédemment collectées dans les cliniques de stérilisation à grand volume en cours pourraient être utilisées pour mesurer les impacts de tels programmes de DPM. Animaux: Nous avons utilisé les données d'admission recueillies de 2008 à 2019 dans des cliniques de stérilisation qui avaient été dispensées à plusieurs reprises sur une période de 10 ans dans 6 communautés des Premières Nations de l'Alberta, pour évaluer les changements dans les caractéristiques d'admission des chiens. Procédures: Le nombre de chiens amenés pour une chirurgie de stérilisation ou cédés, ainsi que leurs âges, sexes, races, poids et scores de condition physique ont été comparés. Les raisons de l'abandon ont été étudiées ainsi que les facteurs socio-économiques en tant que facteurs possibles des différences communautaires dans les taux de participation aux cliniques et les ratios des sexes entre les cliniques. Résultats: Les indicateurs socioéconomiques ne différaient pas entre les 6 communautés participantes; cependant, le nombre total de cliniques demandées par les communautés variait. Au cours des premières années, les chiennes étaient plus susceptibles d'être amenées aux cliniques pour être stérilisées, tandis que dans les années suivantes, davantage de mâles étaient vus. L'âge auquel les animaux ont été amenés pour la castration ou la stérilisation a diminué avec le temps (P < 0,05), mais pas l'âge d'abandon. Les proportions de races très grandes, petites et très petites variaient au fil du temps pour les chiens stérilisés et les chiens abandonnés (P < 0,05), les chiens de grande race étant toujours les plus courants. Cependant, il y a eu une augmentation des races petites et très petites au cours des dernières années de l'étude. Enfin, le statut pondéral, une estimation calculée de la condition corporelle des chiens stérilisés, a augmenté avec le temps au sein des races grandes et moyennes, tandis que le score de condition corporelle des chiens abandonnés est passé de « mince ¼ à « idéal ¼ au fil du temps. Conclusions et pertinence clinique: Ce travail met en évidence le potentiel et les défis liés à l'utilisation des données d'admission pour évaluer les impacts des cliniques de stérilisation qui se déroulent à plusieurs reprises sur de nombreuses années dans les mêmes communautés. L'augmentation globale du statut pondéral, les taux de participation élevés et la réduction de l'âge à l'admission suggèrent des effets positifs des programmes de stérilisation évalués.(Traduit par Dr Serge Messier).
Subject(s)
Population Control , Male , Dogs , Female , Animals , Alberta , Data CollectionABSTRACT
Assessing wildlife health in remote regions requires a multi-faceted approach, which commonly involves convenient samplings and the need of identifying and targeting relevant and informative indicators. We applied a novel wildlife health framework and critically assessed the value of different indicators for understanding the health status and trends of an endangered tundra caribou population. Samples and data from the Dolphin and Union caribou herd were obtained between 2015 and 2021, from community-based surveillance programs and from captured animals. We documented and categorized indicators into health determinants (infectious diseases and trace elements), processes (cortisol, pathology), and health outcomes (pregnancy and body condition). During a recent period of steep population decline, our results indicated a relatively good body condition and pregnancy rates, and decreasing levels of stress, along with a low adult cow survival. We detected multiple factors as potential contributors to the reduced survival, including Brucella suis biovar 4, Erysipelothrix rhusiopathiae and lower hair trace minerals. These results remark the need of targeted studies to improve detection and investigations on caribou mortalities. We also identified differences in health indicators between captured and hunter sampled caribou, highlighting the importance of accounting for sampling biases. This integrative approach that drew on multiple data sources has provided unprecedented knowledge on the health in this herd and highlights the value of documenting individual animal health to understand causes of wildlife declines.
Subject(s)
Erysipelothrix , Reindeer , Pregnancy , Female , Cattle , Animals , Animals, Wild , Pregnancy Rate , Health StatusABSTRACT
Across a species' range, populations are exposed to their local thermal environments, which on an evolutionary scale, may cause adaptative differences among populations. Helminths often have broad geographic ranges and temperature-sensitive life stages but little is known about whether and how local thermal adaptation can influence their response to climate change. We studied the thermal responses of the free-living stages of Marshallagia marshalli, a parasitic nematode of wild ungulates, along a latitudinal gradient. We first determine its distribution in wild sheep species in North America. Then we cultured M. marshalli eggs from different locations at temperatures from 5 to 38°C. We fit performance curves based on the metabolic theory of ecology to determine whether development and mortality showed evidence of local thermal adaptation. We used parameter estimates in life-cycle-based host-parasite models to understand how local thermal responses may influence parasite performance under general and location-specific climate-change projections. We found that M. marshalli has a wide latitudinal and host range, infecting wild sheep species from New Mexico to Yukon. Increases in mortality and development time at higher temperatures were most evident for isolates from northern locations. Accounting for location-specific parasite parameters primarily influenced the magnitude of climate change parasite performance, while accounting for location-specific climates primarily influenced the phenology of parasite performance. Despite differences in development and mortality among M. marshalli populations, when using site-specific climate change projections, there was a similar magnitude of impact on the relative performance of M. marshalli among populations. Climate change is predicted to decrease the expected lifetime reproductive output of M. marshalli in all populations while delaying its seasonal peak by approximately 1 month. Our research suggests that accurate projections of the impacts of climate change on broadly distributed species need to consider local adaptations of organisms together with local temperature profiles and climate projections.
ABSTRACT
Microbiome diversity and diet composition concomitantly influence species health, fitness, immunity, and digestion. In environments where diet varies spatially and temporally, microbiome plasticity may promote rapid host adaptation to available resources. For northern ungulates in particular, metabarcoding of noninvasively collected fecal pellets presents unprecedented insights into their diverse ecological requirements and niches by clarifying the interrelationships of microbiomes, key to deriving nutrients, in context of altered forage availability in changing climates. Muskoxen (Ovibos moschatus) are Arctic-adapted species that experience fluctuating qualities and quantities of vegetation. Geography and seasonality have been noted to influence microbiome composition and diversity in muskoxen, yet it is unclear how their microbiomes intersect with diet. Following observations from other species, we hypothesized increasing diet diversity would result in higher microbiome diversity in muskoxen. We assessed diet composition in muskoxen using three common plant metabarcoding markers and explored correlations with microbiome data. Patterns of dietary diversity and composition were not fully concordant among the markers used, yet all reflected the primary consumption of willows and sedges. Individuals with similar diets had more similar microbiomes, yet in contrast to most literature, yielded negative relationships between microbiome and diet alpha diversity. This negative correlation may reflect the unique capacities of muskoxen to survive solely on high-fiber Arctic forage and provide insight into their resiliency to exploit changing dietary resources in a rapidly warming Arctic altering vegetation diversity.
ABSTRACT
Parasites can impact wildlife populations through their effects on host fitness and survival. The life history strategies of a parasite species can dictate the mechanisms and timing through which it influences the host. However, unravelling this species-specific effect is difficult as parasites generally occur as part of a broader community of co-infecting parasites. Here, we use a unique study system to explore how life histories of different abomasal nematode species may influence host fitness. We examined abomasal nematodes in two adjacent, but isolated, West Greenland caribou (Rangifer tarandus groenlandicus) populations. One herd of caribou were naturally infected with Ostertagia gruehneri, a common and dominant summer nematode of Rangifer sspp., and the other with Marshallagia marshalli (abundant; winter) and Teladorsagia boreoarcticus (less abundant; summer), allowing us to determine if these nematode species have differing effects on host fitness. Using a Partial Least Squares Path Modelling approach, we found that in the caribou infected with O. gruehneri, higher infection intensity was associated with lower body condition, and that animals with lower body condition were less likely to be pregnant. In caribou infected with M. marshalli and T. boreoarcticus, we found that only M. marshalli infection intensity was negatively related to body condition and pregnancy, but that caribou with a calf at heel were more likely to have higher infection intensities of both nematode species. The differing effects of abomasal nematode species on caribou health outcomes in these herds may be due to parasite species-specific seasonal patterns which influence both transmission dynamics and when the parasites have the greatest impact on host condition. These results highlight the importance of considering parasite life history when testing associations between parasitic infection and host fitness.
Subject(s)
Nematoda , Parasites , Reindeer , Trichostrongyloidea , Animals , Female , Pregnancy , Reindeer/parasitology , OstertagiaABSTRACT
In 2016, the first orf virus, a double-stranded DNA (dsDNA) virus of the genus parapoxvirus, from a muskox was isolated on Victoria Island, Nunavut (NU), Canada. We used deep sequencing on DNA extracted from orf virus-positive tissues from wild muskoxen from locations on Victoria Island and the adjacent mainland. Orf virus sequence reads derived from four samples were nearly identical. The consensus sequences generated from pooled reads of MxOV comprises of a large contiguous sequence (contig) of 131,759 bp and a smaller right terminal contig of 3552 bp, containing all coding sequences identified as Parapoxvirus. Individual gene comparisons reveal that MxOV shares genetic characteristics with reference strains from both sheep and goat origin. Recombination analysis using Bootscan, MAXCHI, GENECONV, CHIMAERA, SISCAN, and RDP algorithms within the RDP4 software predicted recombination events in two virulence factors, and a large 3000 bp segment of the MxOV genome. Partial B2L nucleotide sequences from strains around the world and other North American isolates were compared to MxOV using MUSCLE alignments and RAxML phylogenetic trees. MxOV was identical to our previously characterized isolate, and shared similarity with orf virus isolated from sheep and goats. The phylogenetic grouping of partial B2L nucleotide sequences did not follow the sample geographic distribution. More full genomes of orf virus, or at least full B2L gene squences, in wildlife are needed especially in North America to better understand the epidemiology of the disease in muskoxen.
Subject(s)
Communicable Diseases , Orf virus , Sheep , Animals , Phylogeny , Canada/epidemiology , Ruminants , Orf virus/genetics , Goats , High-Throughput Nucleotide SequencingABSTRACT
RATIONALE: Analysis of stable isotopes in tissue and excreta may provide information about animal diets and their nutritional state. As body condition may have a major influence on reproduction, linking stable isotope values to animal demographic rates may help unravel the drivers behind animal population dynamics. METHODS: We performed sequential analysis of δ15 N values in guard hair from 21 muskoxen (Ovibos moschatus) from Zackenberg in high arctic Greenland. We were able to reconstruct the dietary history for the population over a 5-year period with contrasting environmental conditions. We examined the linkage between guard hair δ15 N values in 12 three-month periods and muskox calf recruitment to detect critical periods for muskox reproduction. Finally, we conducted similar analyses of the correlation between environmental conditions (snow depth and air temperature) and calf recruitment. RESULTS: δ15 N values exhibited a clear seasonal pattern with high levels in summer and low levels in winter. However, large inter-annual variation was found in winter values, suggesting varying levels of catabolism depending on snow conditions. In particular δ15 N values during January-March were linked to muskox recruitment rates, with higher values coinciding with lower calf recruitment. δ15 N values were a better predictor of muskox recruitment rates than environmental conditions. CONCLUSIONS: Although environmental conditions may ultimately determine the dietary δ15 N signal in muskox guard hairs, muskox calf recruitment was more strongly correlated with δ15 N values than ambient snow and temperature. The period January-March, corresponding to late gestation, appears particularly critical for muskox reproduction.
Subject(s)
Diet , Ruminants , Animals , Pregnancy , Female , Arctic Regions , Hair , Nutritional StatusABSTRACT
Rotations in diverse, marginalized communities may offer health care students opportunities to develop cultural humility through different clinical experiences and activities. Through the actualization of cultural humility, veterinarians may offer accessible, affordable, culturally proficient, high-quality care to all their patients with a better understanding of how cultural differences affect the animal patient's health, well-being, and care. The purpose of this study was to explore whether participation in a community rotation in remote northern Indigenous communities promotes cultural humility among final-year veterinary students. Small groups of University of Calgary veterinary students travel annually to the Sahtu Settlement Area of the Northwest Territories, Canada, to participate in the Northern Community Health Rotation (NCHR). During the 4-week rotation, students spend 2.5 weeks providing veterinary services to domestic animals in five communities in the Sahtu. Eleven veterinary students who attended the NCHR between 2015 and 2020 answered exploratory open-ended questions in an online survey. Responses highlight areas of learning that contributed to their development of cultural humility. The rotation appears successful in increasing students' confidence working with people from diverse cultures, offering students opportunities to implement a client-centered approach, and advancing their capacity to recognize and challenge their preconceived biases about Indigenous cultures and animal ownership. These experiences are important to the acquisition of cultural humility for veterinary care providers.
Subject(s)
Education, Veterinary , Public Health , Animals , Humans , Public Health/education , Cultural Competency/education , Students , LearningABSTRACT
Climate change is affecting Arctic ecosystems, including parasites. Predicting outcomes for host-parasite systems is challenging due to the complexity of multi-species interactions and the numerous, interacting pathways by which climate change can alter dynamics. Increasing temperatures may lead to faster development of free-living parasite stages but also higher mortality. Interactions between behavioural plasticity of hosts and parasites will also influence transmission processes. We combined laboratory experiments and population modelling to understand the impacts of changing temperatures on barren-ground caribou (Rangifer tarandus) and their common helminth (Ostertagia gruehneri). We experimentally determined the thermal performance curves for mortality and development of free-living parasite stages and applied them in a spatial host-parasite model that also included behaviour of the parasite (propensity for arrested development in the host) and host (long-distance migration). Sensitivity analyses showed that thermal responses had less of an impact on simulated parasite burdens than expected, and the effect differed depending on parasite behaviour. The propensity for arrested development and host migration led to distinct spatio-temporal patterns in infection. These results emphasize the importance of considering behaviour-and behavioural plasticity-when projecting climate-change impacts on host-parasite systems.
ABSTRACT
Several caribou (Rangifer tarandus) populations have been declining concurrently with increases in infectious diseases in the Arctic. Erysipelothrix rhusiopathiae, a zoonotic bacterium, was first described in 2015 as a notable cause of illness and death among several Arctic wildlife species. We investigated epidemiologic and environmental factors associated with the seroprevalence of E. rhusiopathiae in the Arctic and found that seropositivity was highest during warmer months, peaking in September, and was highest among adult males. Summer seroprevalence increases tracked with the oestrid index from the previous year, icing and snowing events, and precipitation from the same year but decreased with growing degree days in the same year. Seroprevalence of E. rhusiopathiae varied more during the later years of the study. Our findings provide key insights into the influence of environmental factors on disease prevalence that can be instrumental for anticipating and mitigating diseases associated with climate change among Arctic wildlife and human populations.
Subject(s)
Erysipelothrix , Reindeer , Animals , Animals, Wild , Arctic Regions , Humans , Male , Seasons , Seroepidemiologic StudiesABSTRACT
Orthostrongylus macrotis (Dikmans, 1931) is a protostrongylid lungworm in wild ungulates from western North America, including mule and Columbia black-tailed deer, pronghorn, and rarely moose and elk. The lack of morphological data for certain developmental stages of O. macrotis and the unresolved taxonomic status of the genus indicate a more detailed morphological characterization of the species is necessary. We provide a detailed description of first-stage larvae (L1) of O. macrotis including morphological, morphometric, and molecular data. Species identity was confirmed based on molecular sequence data from the internal transcribed spacer subunit 2 (ITS-2) and large subunit (28S) rDNA. A fragment of the cytochrome oxidase c subunit 1 (COI) was also sequenced, followed by the determination of genetic distance and phylogenetic analyses. Integrated data describing L1 of O. macrotis contributes to a broader understanding of the parasite fauna of wild ungulates from North America and may be of relevance for a future revision of the genus. Further, we outline information for differentiation among species of North American protostrongylids, with typical spike-tailed L1s, circulating among free-ranging and semi-domestic ungulates.
Subject(s)
Deer , Metastrongyloidea , Strongylida Infections , Animals , DNA, Ribosomal Spacer/genetics , Deer/parasitology , Larva/genetics , North America , Phylogeny , Strongylida Infections/parasitology , Strongylida Infections/veterinaryABSTRACT
The role and impact of infectious diseases in wildlife population dynamics are increasingly recognized, yet disease information is variably incorporated into wildlife management frameworks. This discrepancy is particularly relevant for Rangifer tarandus (caribou or reindeer), a keystone circumarctic species experiencing widespread population declines. The primary objective of this review was to characterize the available peer-reviewed literature on infectious diseases of Rangifer by using a scoping review methodology. Three databases of peer-reviewed literature-Web of Science, BIOSIS previews, and Scopus-were searched and 695 articles met the criteria for initial review. After screening for relevance and language, 349 articles, published between 1967 and 2020, remained. More than half of the excluded articles (181/346; 52%) were left out because they were not published in English; the majority of these excluded articles (120) were in Russian. From the 349 included articles, 137 (39%) pertained to wild (as opposed to semidomesticated or captive) Rangifer populations. Articles on infectious disease in wild Rangifer were published in 40 different journals across various disciplines; the most common journals were disease and parasitology oriented, accounting for 55% of included articles. Most studies were descriptive (87%), followed by experimental (9%). Of the pathogen taxa investigated, helminths were the most common, comprising 35% of articles. Rangifer subspecies were not equally represented in the literature, with barren-ground caribou (R. t. groenlandicus; n=40) and woodland caribou (R. t. caribou; n=39) having the greatest abundance and diversity of infectious disease information available. Few studies explicitly examined individual or population-level impacts of disease, or related disease to vital population rates, and only 27 articles explicitly related results to management or conservation. Findings from this review highlight an unbalanced distribution of studies across Rangifer ecotypes, a preference for dissemination in disease-specialized publication venues, and an opportunity for investigating population-level impacts that may be more readily integrated into caribou conservation frameworks.