ABSTRACT
The study of the action of the electromagnetic radiation (EMR) of low intensity (10 microW/cm2) in the range of frequencies 120-170 GHz at the test-reaction of Infusoria Paramecium caudatum was carried out. The resonant character of the effects was established. The EMR action at 156.6 and 161.3 GHz caused the increase of infusorians mobility, the action at frequencies 151.8, 155.7, 167.1 GHz caused the mobility reduction. Isolated and combined with EMR effects of nicotine (10(-4)-10(-15) mol/l) and antimicrobial drug metronidazole (10(-5), 10(-8), 10(-9) mol/l) were investigated. The radiation at the frequency 167.1 GHz was shown to reduce the effect of nicotine (10(-9) mol/l) and to enhance the effect of metronidazole (10(-9) mol/l). This phenomenon may be explained by different effects of the substances in low concentration at the water hydrogen bonds net structure.
Subject(s)
Antiprotozoal Agents/pharmacology , Metronidazole/pharmacology , Microwaves , Nicotine/pharmacology , Paramecium caudatum/drug effects , Paramecium caudatum/radiation effects , Animals , Cell Movement/drug effects , Cell Movement/radiation effects , Dose-Response Relationship, Drug , Dose-Response Relationship, Radiation , Hydrogen Bonding/drug effects , Hydrogen Bonding/radiation effects , Time FactorsABSTRACT
Metronidazolum decreased diffusion mobility of membrane surface water. It is possible, that Wuks's effect represents the molecular mechanism underlying this phenomenon. We suppose, that protective action of the derivatives of the nitroimidazole on cell lysis is related to changes of the diffusion mobility of the surface water.
Subject(s)
Antiprotozoal Agents/metabolism , Antiprotozoal Agents/pharmacology , Cell Membrane Permeability/drug effects , Erythrocyte Membrane/metabolism , Metronidazole/pharmacology , Water/metabolism , Animals , Diffusion , Male , RatsABSTRACT
The effect of low morphine concentrations on the plasmatic membranes of erythrocytes without opiate receptors was investigated. It was shown that the ATPase activity and hemolytic stability of erythrocytes, which characterize the state of cell membranes and the mobility of the near-membrane water phase, depend on the concentration of morphine, and this dependence is wave-like. The nonmonotonous dependence of the biological response was suggested to be due to changes in the structure of water hydrogen links near the membrane surface, induced by opiate molecules. The hypothesis was confirmed by the results of studies of morphine water solutions using the methods of fluorescent probe and light scattering. It was found that the intensity of light scattering by water and the mobility of its molecules considerably increase in the presence of strictly specified concentrations of morphine.