Subject(s)
Humans , Female , Young Adult , Adult , Superior Mesenteric Artery Syndrome/surgery , Duodenostomy/methods , Laparoscopy/methods , Treatment OutcomeABSTRACT
No disponible
Subject(s)
Humans , Male , Middle Aged , Suicide, Attempted , Neck Injuries/surgery , Surgery, Computer-Assisted , Schizophrenia/complications , Wounds, Penetrating/surgerySubject(s)
Neck Injuries/etiology , Suicide, Attempted , Wounds, Penetrating/etiology , Humans , Male , Middle AgedABSTRACT
Hepatocellular carcinoma develops in cirrhotic liver. The nitric oxide (NO) synthase type III (NOS-3) overexpression induces cell death in hepatoblastoma cells. The study developed gene therapy designed to specifically overexpress NOS-3 in cultured hepatoma cells, and in tumors derived from orthotopically implanted tumor cells in fibrotic livers. Liver fibrosis was induced by CCl4 administration in mice. The first-generation adenoviruses were designed to overexpress NOS-3 or green fluorescent protein, and luciferase complementary DNA under the regulation of murine alpha-fetoprotein (AFP) and Rous Sarcoma Virus (RSV) promoters, respectively. Both adenovirus and Hepa 1-6 cells were used for in vitro and in vivo experiments. Adenoviruses were administered through the tail vein 2 weeks after orthotopic tumor cell implantation. AFP-NOS-3/RSV-luciferase increased oxidative-related DNA damage, p53, CD95/CD95L expression and caspase-8, -9 and -3 activities in cultured Hepa 1-6 cells. The increased expression of CD95/CD95L and caspase-8 activity was abolished by Nω-nitro-l-arginine methyl ester hydrochloride, p53 and CD95 small interfering RNA. AFP-NOS-3/RSV-luciferase adenovirus increased cell death markers, and reduced cell proliferation of established tumors in fibrotic livers. The increase of oxidative/nitrosative stress induced by NOS-3 overexpression induced DNA damage, p53, CD95/CD95L expression and cell death in hepatocellular carcinoma cells. The effectiveness of the gene therapy has been demonstrated in vitro and in vivo.
Subject(s)
Carcinoma, Hepatocellular/therapy , Gene Expression Regulation, Neoplastic , Genetic Therapy/methods , Liver Neoplasms/therapy , Nitric Oxide Synthase Type III/genetics , Adenoviridae/genetics , Animals , Carcinoma, Hepatocellular/genetics , Caspase 3/genetics , Caspase 3/metabolism , Caspase 8/genetics , Caspase 8/metabolism , Caspase 9/genetics , Caspase 9/metabolism , Cell Line, Tumor , Cell Proliferation , DNA Damage , DNA, Complementary/genetics , DNA, Complementary/metabolism , Disease Models, Animal , Fas Ligand Protein/genetics , Fas Ligand Protein/metabolism , Genetic Vectors , Liver/cytology , Liver/metabolism , Liver Cirrhosis/genetics , Liver Cirrhosis/therapy , Liver Neoplasms/genetics , Mice , NG-Nitroarginine Methyl Ester/metabolism , Nitric Oxide Synthase Type III/metabolism , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Rous sarcoma virus/genetics , Tumor Suppressor Protein p53/genetics , Tumor Suppressor Protein p53/metabolism , alpha-Fetoproteins/genetics , alpha-Fetoproteins/metabolismSubject(s)
Humans , Male , Adult , Intestinal Perforation/complications , Intestinal Perforation/diagnosis , Intestinal Perforation/surgery , Foreign Bodies/complications , Foreign Bodies/diagnosis , Foreign Bodies/surgery , Colostomy/methods , Surgical Wound Infection/complications , Surgical Wound Infection/diagnosis , Surgical Wound Infection/therapy , Intestinal Perforation , Abdominal Pain/complications , Abdominal Pain/etiology , Colon/injuries , Colon/pathology , Colon/surgery , Colorectal Surgery/methodsSubject(s)
Humans , Female , Middle Aged , Fibroma/diagnosis , Fibroma/surgery , Mesentery/pathology , Peritoneal Neoplasms/diagnosis , Peritoneal Neoplasms/surgery , Cholecystectomy/methods , Acute Disease , Cholecystitis/surgery , Postoperative Complications/diagnosis , Postoperative Complications/surgery , Leukocytosis/complications , Radiography, Abdominal/methodsSubject(s)
Gallstones/complications , Ileal Diseases/etiology , Ileus/etiology , Aged , Female , Humans , RecurrenceABSTRACT
No disponible
Subject(s)
Humans , Male , Middle Aged , Abdominal Wall , Cholecystectomy, Laparoscopic/adverse effects , Epigastric Arteries/injuries , Hematoma/etiology , Hematoma , Radiography, Abdominal , Surgical Instruments/adverse effects , Tomography, X-Ray ComputedSubject(s)
Abdominal Wall , Cholecystectomy, Laparoscopic/adverse effects , Epigastric Arteries/injuries , Hematoma/diagnostic imaging , Hematoma/etiology , Radiography, Abdominal , Surgical Instruments/adverse effects , Tomography, X-Ray Computed , Diabetes Mellitus, Type 2/complications , Humans , Male , Middle AgedABSTRACT
La sospecha clínica de tuberculosis peritoneal debe estar presenteen todo paciente con dolor abdominal de etiología desconocida;sobre todo si se acompaña de fiebre, ascitis y distensión abdominal.El acceso por vía laparoscópica a la cavidad abdominalde forma reglada contribuye de manera primordial al diagnósticotanto por la imagen macroscópica como para la toma de biopsia,que dará posteriormente la confirmación anatomopatológica ymicrobiológica. Ayudando a discriminar entre los posibles diagnósticosdiferenciales que acontecen con clínica similar. Otraspruebas diagnósticas analíticas deben ser tenidas en cuenta paraayudar tanto a la indicación de laparoscopia como de cara al diagnóstico,son tales como la ADA, gammagrafía con Galio-67 y Ca-125
The presence of peritoneal tuberculosis has to be clinically suspectedin all patients with abdominal pain of unknown etiology,particularly when it is accompanied by fever, ascites, and abdominaldistension. Access to the abdominal cavity using routine laparoscopyprovides essential information on the diagnosis, fromboth macroscopic images and biopsy sampling, which will laterprovide a pathological and microbiological confirmation. Thishelps discriminate between potential differential diagnoses thatmay include similar symptoms. Other laboratory tests have to beconsidered as diagnostic aids, as well as for the indication of laparoscopy,including ADA, and Gallium-67 or Ca-125 scans (AU)
Subject(s)
Humans , Female , Adult , Peritonitis, Tuberculous/diagnosis , Laparoscopy , Abdominal Pain/etiology , Ascites/etiology , Diagnosis, DifferentialABSTRACT
No disponible
Subject(s)
Humans , Female , Aged , Cutaneous Fistula/diagnosis , Intestinal Fistula/diagnosis , Ileal Diseases/diagnosis , Hernia, Umbilical/complications , Intestinal Perforation/complicationsABSTRACT
The presence of peritoneal tuberculosis has to be clinically suspected in all patients with abdominal pain of unknown etiology, particularly when it is accompanied by fever, ascites, and abdominal distension. Access to the abdominal cavity using routine laparoscopy provides essential information on the diagnosis, from both macroscopic images and biopsy sampling, which will later provide a pathological and microbiological confirmation. This helps discriminate between potential differential diagnoses that may include similar symptoms. Other laboratory tests have to be considered as diagnostic aids, as well as for the indication of laparoscopy, including ADA, and Gallium-67 or Ca-125 scans.
Subject(s)
Laparoscopy , Peritonitis, Tuberculous/diagnosis , Female , Humans , Middle AgedSubject(s)
Aneurysm, False/etiology , Drainage/adverse effects , Hepatic Artery , Aged, 80 and over , Bile Ducts , Drainage/methods , Female , HumansABSTRACT
Hepaticojejunostomy is a good alternative technique for biliary reconstruction in liver transplantation. Among 517 liver transplants performed between March 1992 and July 2005, 33 involved hepaticojejunostomy, namely, 18 men and 12 women of average age: 44.8 years. The main cause for this technique was retransplant (n = 10), secondary biliary cirrhosis (n = 5), alcoholic cirrhosis (n = 5), HCV cirrhosis (n = 2), primary biliary cirrhosis (n = 1), cryptogenic cirrhosis (n = 1), sclerosing cholangitis (n = 3), fulminant liver failure (n = 1), autoimmune cirrhosis (n = 1), and insulinoma metastasis (n = 1). Choledochojejunostomy was performed for all Roux-en-Y loops, with an average cold ischemia time of 361.16 minutes (180-780). The biliary complications were biliary fistula in four cases (13.3%), including two who required surgery; stenosis of the anastomosis in two cases (6.6%) including one diagnosed by HIDA that resolved with medical treatment and the other, diagnosed by cholangio-MRI, requiring a new hepaticojejunostomy; and biliary peritonitis in three cases (10%), all of whom required surgery. The vascular complications were thrombosis of the hepatic artery (n = 1), which required retransplantation, and pseudoaneurysm of hepatic artery (n = 1). No biliary complications occurred. The 6-month patient survival was 80% and the 6-month graft survival was 77%; no patient died due to biliary complications. Hepaticojejunostomy is a technique with higher morbidity than choledocho-choledochostomy, but it is the best alternative when the latter is not possible.