Fecal DNA metabarcoding helps characterize the Canada jay's diet and confirms its reliance on stored food for winter survival and breeding.

Accurately determining the diet of wild animals can be challenging if food items are small, visible only briefly, or rendered visually unidentifiable in the digestive system. In some food caching species, an additional challenge is determining whether consumed diet items have been previously stored or are fresh. The Canada jay (Perisoreus canadensis) is a generalist resident of North American boreal and subalpine forests with anatomical and behavioural adaptations allowing it to make thousands of arboreal food caches in summer and fall that are presumably responsible for its high winter survival and late winter/early spring breeding. We used DNA fecal metabarcoding to obtain novel information on nestling diets and compiled a dataset of 662 published and unpublished direct observations or stomach contents identifications of natural foods consumed by Canada jays throughout the year. We then used detailed natural history information to make informed decisions on whether each item identified to species in the diets of winter adults and nestlings was best characterized as 'likely cached', 'likely fresh' (i.e., was available as a non-cached item when it appeared in a jay's feces or stomach), or 'either possible'. Of the 87 food items consumed by adults in the winter, 39% were classified as 'likely cached' and 6% were deemed to be 'likely fresh'. For nestlings, 29% of 125 food items identified to species were 'likely cached' and 38% were 'likely fresh'. Our results support both the indispensability of cached food for Canada jay winter survival and previous suggestions that cached food is important for late winter/early spring breeding. Our work highlights the value of combining metabarcoding, stomach contents analysis, and direct observations to determine the cached vs. non-cached origins of consumed food items and the identity of food caches, some of which could be especially vulnerable to degradation through climate change.

A microscale three-dimensional model of urban outdoor thermal exposure (TUF-Pedestrian).

Urban street design choices relating to tree planting, building height and spacing, ground cover, and building façade properties impact outdoor thermal exposure. However, existing tools to simulate heat exposure have limitations with regard to optimization of street design for pedestrian cooling. A microscale three-dimensional (3D) urban radiation and energy balance model, Temperatures of Urban Facets for Pedestrians (TUF-Pedestrian), was developed to simulate pedestrian radiation exposure and study heat-reducing interventions such as urban tree planting and modifications to building and paving materials. TUF-Pedestrian simulates the spatial distribution of radiation and surface temperature impacts of trees and buildings on their surroundings at the sub-facet scale. In addition, radiation absorption by a three-dimensional pedestrian is considered, permitting calculation of a summary metric of human radiation exposure: the mean radiant temperature (TMRT). TUF-Pedestrian is evaluated against a unique 24-h observational dataset acquired using a mobile human-biometeorological station, MaRTy, in an urban canyon with trees on the Arizona State University Tempe campus (USA). Model evaluation demonstrates that TUF-Pedestrian accurately simulates both incoming directional radiative fluxes and TMRT in an urban environment with and without tree cover. Model sensitivity simulations demonstrate how modelled TMRT and directional radiative fluxes respond to increased building height (ΔTMRT reaching -32 °C when pedestrian becomes shaded), added tree cover (ΔTMRT approaching -20 °C for 8 m trees with leaf area density of 0.5 m2 m-3), and increased street albedo (ΔTMRT reaching + 6 °C for a 0.21 increase in pavement albedo). Sensitivity results agree with findings from previous studies and demonstrate the potential utility of TUF-Pedestrian as a tool to optimize street design for pedestrian heat exposure reduction.