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PLoS One ; 10(9): e0139284, 2015.
Article in English | MEDLINE | ID: mdl-26402555

ABSTRACT

Kir7.1 is an inwardly rectifying K+ channel of the Kir superfamily encoded by the kcnj13 gene. Kir7.1 is present in epithelial tissues where it colocalizes with the Na+/K+-pump probably serving to recycle K+ taken up by the pump. Human mutations affecting Kir7.1 are associated with retinal degeneration diseases. We generated a mouse lacking Kir7.1 by ablation of the Kcnj13 gene. Homozygous mutant null mice die hours after birth and show cleft palate and moderate retardation in lung development. Kir7.1 is expressed in the epithelium covering the palatal processes at the time at which palate sealing takes place and our results suggest it might play an essential role in late palatogenesis. Our work also reveals a second unexpected role in the development and the physiology of the respiratory system, where Kir7.1 is expressed in epithelial cells all along the respiratory tree.


Subject(s)
Cleft Palate/pathology , Lung/growth & development , Lung/pathology , Potassium Channels, Inwardly Rectifying/deficiency , Animals , Animals, Newborn , Body Weight , Cleft Palate/embryology , Embryonic Development , Epithelium/metabolism , Epithelium/pathology , Lung/abnormalities , Lung/embryology , Mice, Inbred C57BL , Mice, Mutant Strains , Potassium Channels, Inwardly Rectifying/metabolism , Survival Analysis
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