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1.
Int J Biol Macromol ; 271(Pt 2): 132714, 2024 May 28.
Article in English | MEDLINE | ID: mdl-38815937

ABSTRACT

OBJECTIVES: The study aimed to identify a quantitative signature of circulating small non-coding RNAs (sncRNAs) as a biomarker for pulmonary tuberculosis disease (active-TB/ATB) and explore their regulatory roles in host-pathogen interactions and disease progression. METHODS: We conducted a cross-sectional study recruiting subjects diagnosed with active-TB (drug-sensitive and drug-resistant) and healthy controls. Sera samples were collected and utilized for preparing small RNA libraries. Quantitative patterns of circulating sncRNAs (miRNAs, piRNAs and tRFs) were identified via high-throughput sequencing and DeSeq2 analysis and validated in independent active-TB cohorts. Functional knockdown for two selected miRNAs were also performed. RESULTS: A diagnostic signature of four sncRNAs for both drug-sensitive and drug-resistant active-TB cases was validated, exhibiting an AUC of 0.96 (95% CI: 0.937-0.996, p < 0.001) with 86.7% sensitivity (95% CI: 0.775-0.932) and 91.7% specificity (95% CI: 0.730-0.990) in ROC analysis. Functional knockdown demonstrated regulatory roles of hsa-miR-223-5p and hsa-miR-10b-5p in Mycobacterium tuberculosis (Mtb) growth and pro-inflammatory cytokine expression (IL-6 and IL-8). CONCLUSION: The study identified a diagnostic tool utilizing a signature of four sncRNAs with high specificity and sensitivity, enhancing our understanding of sncRNAs as ATB diagnostic biomarker. Additionally, hsa-miR-223-5p and hsa-miR-10b-5p demonstrated potential roles in Mtb pathogenesis and host-response to infection.

2.
J Cell Sci ; 135(20)2022 10 15.
Article in English | MEDLINE | ID: mdl-36172824

ABSTRACT

Extracellular matrix (ECM) is an important component of stem cell niche. Remodeling of ECM mediated by ECM regulators, such as matrix metalloproteinases (MMPs) plays a vital role in stem cell function. However, the mechanisms that modulate the function of ECM regulators in the stem cell niche are understudied. Here, we explored the role of the transcription factor (TF) ETS-1, which is expressed in the cathepsin-positive cell population, in regulating the expression of the ECM regulator, mt-mmpA, thereby modulating basement membrane thickness. In planarians, the basement membrane around the gut/inner parenchyma is thought to act as a niche for pluripotent stem cells. It has been shown that the early epidermal progenitors migrate outwards from this region and progressively differentiate to maintain the terminal epidermis. Our data shows that thickening of the basement membrane in the absence of ets-1 results in defective migration of stem cell progeny. Furthermore, the absence of ets-1 leads to a defective epidermal progenitor landscape, despite its lack of expression in those cell types. Together, our results demonstrate the active role of ECM remodeling in regulating tissue homeostasis and regeneration in the planarian Schmidtea mediterranea. This article has an associated First Person interview with one of the co-first authors of the paper.


Subject(s)
Mediterranea , Planarians , Animals , Humans , Cell Differentiation , Cathepsins/metabolism , Planarians/metabolism , Epidermis/metabolism , Matrix Metalloproteinases/metabolism , Basement Membrane/metabolism , Transcription Factors/metabolism
3.
Nat Genet ; 54(7): 963-975, 2022 07.
Article in English | MEDLINE | ID: mdl-35773407

ABSTRACT

The consensus molecular subtype (CMS) classification of colorectal cancer is based on bulk transcriptomics. The underlying epithelial cell diversity remains unclear. We analyzed 373,058 single-cell transcriptomes from 63 patients, focusing on 49,155 epithelial cells. We identified a pervasive genetic and transcriptomic dichotomy of malignant cells, based on distinct gene expression, DNA copy number and gene regulatory network. We recapitulated these subtypes in bulk transcriptomes from 3,614 patients. The two intrinsic subtypes, iCMS2 and iCMS3, refine CMS. iCMS3 comprises microsatellite unstable (MSI-H) cancers and one-third of microsatellite-stable (MSS) tumors. iCMS3 MSS cancers are transcriptomically more similar to MSI-H cancers than to other MSS cancers. CMS4 cancers had either iCMS2 or iCMS3 epithelium; the latter had the worst prognosis. We defined the intrinsic epithelial axis of colorectal cancer and propose a refined 'IMF' classification with five subtypes, combining intrinsic epithelial subtype (I), microsatellite instability status (M) and fibrosis (F).


Subject(s)
Colorectal Neoplasms , Neoplasms, Glandular and Epithelial , Colorectal Neoplasms/genetics , Colorectal Neoplasms/pathology , Epithelial Cells/pathology , Humans , Microsatellite Instability , Microsatellite Repeats/genetics , Neoplasms, Glandular and Epithelial/genetics , Transcriptome/genetics
4.
Dev Biol ; 488: 11-29, 2022 08.
Article in English | MEDLINE | ID: mdl-35523320

ABSTRACT

Planarians have a remarkable ability to undergo whole-body regeneration. Successful regeneration outcome is determined by processes like polarity establishment at the wound site, which is followed by pole (organizer) specification. Interestingly, these determinants are almost exclusively expressed by muscles in these animals. However, the molecular toolkit that enables the functional versatility of planarian muscles remains poorly understood. Here we report that SMED_DDX24, a D-E-A-D Box RNA helicase, is necessary for planarian survival and regeneration. We found that DDX24 is enriched in muscles and its knockdown disrupts muscle fiber organization. This leads to defective pole specification, which in turn results in misregulation of many positional control genes specifically during regeneration. ddx24 RNAi also upregulates wound-induced Wnt signalling. Suppressing this ectopic Wnt activity rescues the knockdown phenotype by enabling better anterior pole regeneration. To summarize, our work highlights the role of an RNA helicase in muscle fiber organization, and modulating amputation-induced wnt levels, both of which seem critical for pole re-organization, thereby regulating whole-body regeneration.


Subject(s)
Planarians , Animals , Body Patterning/genetics , Muscle Fibers, Skeletal/metabolism , Planarians/physiology , RNA Helicases , RNA Interference , Signal Transduction/genetics , Wnt Proteins/metabolism
5.
Glycobiology ; 32(1): 36-49, 2022 02 26.
Article in English | MEDLINE | ID: mdl-34499167

ABSTRACT

O-Glycans on cell surfaces play important roles in cell-cell, cell-matrix and receptor-ligand interaction. Therefore, glycan-based interactions are important for tissue regeneration and homeostasis. Free-living flatworm Schmidtea mediterranea, because of its robust regenerative potential, is of great interest in the field of stem cell biology and tissue regeneration. Nevertheless, information on the composition and structure of O-glycans in planaria is unknown. Using mass spectrometry and in silico approaches, we characterized the glycome and the related transcriptome of mucin-type O-glycans of planarian S. mediterranea. Mucin-type O-glycans were composed of multiple isomeric, methylated, and unusually extended mono- and disubstituted O-N-acetylgalactosamine structures. Extensions made of hexoses and 3-O-methyl hexoses were the glycoforms observed. From glycotranscriptomic analysis, 60 genes belonging to five distinct enzyme classes were identified to be involved in mucin-type O-glycan biosynthesis. These genes shared homology with those in other invertebrate systems. Although a majority of the genes involved in mucin-type O-glycan biosynthesis were highly expressed during organogenesis and in differentiated cells, a few select genes in each enzyme class were specifically enriched during early embryogenesis. Our results indicate a unique temporal and spatial role for mucin-type O-glycans during embryogenesis and organogenesis and in adulthood. In summary, this is the first report on O-glycans in planaria. This study expands the structural and biosynthetic possibilities in cellular glycosylation in the invertebrate glycome and provides a framework towards understanding the biological role of mucin-type O-glycans in tissue regeneration using planarians.


Subject(s)
Planarians , Animals , Glycomics , Mediterranea , Mucins/metabolism , Planarians/genetics , Planarians/metabolism , Polysaccharides/chemistry
6.
Front Immunol ; 12: 718005, 2021.
Article in English | MEDLINE | ID: mdl-34721382

ABSTRACT

Macrophages are highly responsive to the environmental cues and are the primary responders to tissue stress and damage. While much is known about the role of macrophages during inflammatory disease progression; the initial series of events that set up the inflammation remains less understood. In this study, we use next generation sequencing (NGS) of embryonic skin macrophages and the niche cells - skin epithelia and stroma in the epidermis specific knockout of integrin beta 1 (Itgß1) model to uncover specific roles of each cell type and identify how these cell types communicate to initiate the sterile inflammatory response. We demonstrate that while the embryonic skin fibroblasts in the Itgß1 knockout skin are relatively inactive, the keratinocytes and macrophages are the critical responders to the sterile inflammatory cues. The epidermis expresses damage associated molecular patterns (DAMPs), stress response genes, pro-inflammatory cytokines, and chemokines that aid in eliciting the inflammatory response. The macrophages, in-turn, respond by acquiring enhanced M2-like characteristics expressing ECM remodeling and matrisome signatures that exacerbate the basement membrane disruption. Depletion of macrophages by blocking the CSF1 receptor (CSF1R) results in improved basement membrane integrity and reduced ECM remodeling activity in the KO skin. Further, blocking the skin inflammation with celecoxib reveals that the acquired fate of macrophages in the KO skin is dependent on its interaction with the epidermal compartment through COX2 dependent cytokine production. Taken together, our study highlights a critical crosstalk between the epithelia and the dermal macrophages that shapes macrophage fate and initiates sterile inflammation in the skin. The insights gained from our study can be extrapolated to other inflammatory disorders to understand the early events that set up the disease.


Subject(s)
Dermatitis/etiology , Dermatitis/metabolism , Disease Susceptibility , Epithelium/metabolism , Macrophages/metabolism , Animals , Biomarkers , Epidermis/metabolism , Epidermis/pathology , Extracellular Matrix/metabolism , Female , Fetus , Flow Cytometry , Fluorescent Antibody Technique , Immunohistochemistry , Male , Mice , Mice, Knockout , Models, Biological
7.
Proc Natl Acad Sci U S A ; 118(20)2021 05 18.
Article in English | MEDLINE | ID: mdl-33941643

ABSTRACT

The ability to respond to light has profoundly shaped life. Animals with eyes overwhelmingly rely on their visual circuits for mediating light-induced coordinated movements. Building on previously reported behaviors, we report the discovery of an organized, eye-independent (extraocular), body-wide photosensory framework that allows even a head-removed animal to move like an intact animal. Despite possessing sensitive cerebral eyes and a centralized brain that controls most behaviors, head-removed planarians show acute, coordinated ultraviolet-A (UV-A) aversive phototaxis. We find this eye-brain-independent phototaxis is mediated by two noncanonical rhabdomeric opsins, the first known function for this newly classified opsin-clade. We uncover a unique array of dual-opsin-expressing photoreceptor cells that line the periphery of animal body, are proximal to a body-wide nerve net, and mediate UV-A phototaxis by engaging multiple modes of locomotion. Unlike embryonically developing cerebral eyes that are functional when animals hatch, the body-wide photosensory array matures postembryonically in "adult-like animals." Notably, apart from head-removed phototaxis, the body-wide, extraocular sensory organization also impacts physiology of intact animals. Low-dose UV-A, but not visible light (ocular-stimulus), is able to arouse intact worms that have naturally cycled to an inactive/rest-like state. This wavelength selective, low-light arousal of resting animals is noncanonical-opsin dependent but eye independent. Our discovery of an autonomous, multifunctional, late-maturing, organized body-wide photosensory system establishes a paradigm in sensory biology and evolution of light sensing.


Subject(s)
Brain/metabolism , Eye/metabolism , Helminth Proteins/genetics , Opsins/genetics , Photoreceptor Cells, Invertebrate/metabolism , Planarians/genetics , Animals , Arousal/genetics , Arousal/physiology , Arousal/radiation effects , Brain/growth & development , Eye/growth & development , Gene Expression Profiling/methods , Gene Expression Regulation, Developmental , Helminth Proteins/classification , Helminth Proteins/metabolism , In Situ Hybridization, Fluorescence/methods , Locomotion/genetics , Locomotion/physiology , Locomotion/radiation effects , Movement/physiology , Movement/radiation effects , Opsins/classification , Opsins/metabolism , Phylogeny , Planarians/growth & development , Planarians/metabolism , RNA Interference , Ultraviolet Rays
8.
Stem Cell Reports ; 16(5): 1302-1316, 2021 05 11.
Article in English | MEDLINE | ID: mdl-33861990

ABSTRACT

Mitochondrial state changes were shown to be critical for stem cell function. However, variation in the mitochondrial content in stem cells and the implication, if any, on differentiation is poorly understood. Here, using cellular and molecular studies, we show that the planarian pluripotent stem cells (PSCs) have low mitochondrial mass compared with their progenitors. Transplantation experiments provided functional validation that neoblasts with low mitochondrial mass are the true PSCs. Further, the mitochondrial mass correlated with OxPhos and inhibiting the transition to OxPhos dependent metabolism in cultured cells resulted in higher PSCs. In summary, we show that low mitochondrial mass is a hallmark of PSCs in planaria and provide a mechanism to isolate live, functionally active, PSCs from different cell cycle stages (G0/G1 and S, G2/M). Our study demonstrates that the change in mitochondrial metabolism, a feature of PSCs is conserved in planaria and highlights its role in organismal regeneration.


Subject(s)
Mitochondria/metabolism , Planarians/cytology , Planarians/metabolism , Stem Cells/metabolism , Animals , Cell Differentiation/genetics , Membrane Potential, Mitochondrial , Planarians/genetics , Pluripotent Stem Cells/cytology , Pluripotent Stem Cells/metabolism , RNA, Small Interfering/metabolism , RNA-Seq , Staining and Labeling , Stem Cell Transplantation , Stem Cells/cytology , Transcriptome/genetics
9.
Dev Cell ; 56(6): 761-780.e7, 2021 03 22.
Article in English | MEDLINE | ID: mdl-33725480

ABSTRACT

Vinculin, a mechanotransducer associated with both adherens junctions (AJs) and focal adhesions (FAs), plays a central role in force transmission through cell-cell and cell-substratum contacts. We generated the conditional knockout (cKO) of vinculin in murine skin that results in the loss of bulge stem cell (BuSC) quiescence and promotes continual cycling of the hair follicles. Surprisingly, we find that the AJs in vinculin cKO cells are mechanically weak and impaired in force generation despite increased junctional expression of E-cadherin and α-catenin. Mechanistically, we demonstrate that vinculin functions by keeping α-catenin in a stretched/open conformation, which in turn regulates the retention of YAP1, another potent mechanotransducer and regulator of cell proliferation, at the AJs. Altogether, our data provide mechanistic insights into the hitherto-unexplored regulatory link between the mechanical stability of cell junctions and contact-inhibition-mediated maintenance of BuSC quiescence.


Subject(s)
Adaptor Proteins, Signal Transducing/metabolism , Adherens Junctions/physiology , Hair Follicle/physiology , Mechanotransduction, Cellular , Stem Cells/physiology , Vinculin/physiology , alpha Catenin/metabolism , Adaptor Proteins, Signal Transducing/genetics , Animals , Cell Adhesion , Female , Hair Follicle/cytology , Male , Membrane Potentials , Mice , Mice, Inbred C57BL , Mice, Knockout , Stem Cells/cytology , YAP-Signaling Proteins , alpha Catenin/genetics
10.
RNA ; 27(4): 477-495, 2021 04.
Article in English | MEDLINE | ID: mdl-33446492

ABSTRACT

tRNA-derived fragments (tRFs) have recently gained a lot of scientific interest due to their diverse regulatory roles in several cellular processes. However, their function in dynamic biological processes such as development and regeneration remains unexplored. Here, we show that tRFs are dynamically expressed during planarian regeneration, suggesting a possible role for these small RNAs in the regulation of regeneration. In order to characterize planarian tRFs, we first annotated 457 tRNAs in S. mediterranea combining two tRNA prediction algorithms. Annotation of tRNAs facilitated the identification of three main species of tRFs in planarians-the shorter tRF-5s and itRFs, and the abundantly expressed 5'-tsRNAs. Spatial profiling of tRFs in sequential transverse sections of planarians revealed diverse expression patterns of these small RNAs, including those that are enriched in the head and pharyngeal regions. Expression analysis of these tRF species revealed dynamic expression of these small RNAs over the course of regeneration suggesting an important role in planarian anterior and posterior regeneration. Finally, we show that 5'-tsRNA in planaria interact with all three SMEDWI proteins and an involvement of AGO1 in the processing of itRFs. In summary, our findings implicate a novel role for tRFs in planarian regeneration, highlighting their importance in regulating complex systemic processes. Our study adds to the catalog of posttranscriptional regulatory systems in planaria, providing valuable insights on the biogenesis and the function of tRFs in neoblasts and planarian regeneration.


Subject(s)
Argonaute Proteins/genetics , Helminth Proteins/genetics , Planarians/genetics , RNA, Helminth/genetics , RNA, Small Untranslated/genetics , RNA, Transfer/genetics , Regeneration/genetics , Algorithms , Animals , Argonaute Proteins/metabolism , Base Pairing , Base Sequence , Gene Expression Regulation , Helminth Proteins/metabolism , Molecular Sequence Annotation , Nucleic Acid Conformation , Planarians/metabolism , RNA, Helminth/chemistry , RNA, Helminth/classification , RNA, Helminth/metabolism , RNA, Small Untranslated/chemistry , RNA, Small Untranslated/classification , RNA, Small Untranslated/metabolism , RNA, Transfer/chemistry , RNA, Transfer/classification , RNA, Transfer/metabolism
11.
Dev Dyn ; 249(11): 1347-1364, 2020 11.
Article in English | MEDLINE | ID: mdl-32427396

ABSTRACT

BACKGROUND: Vertebrate cranial neural crest cells (CNCCs) are multipotent, proximal to the source CNCC form the cranial ganglia. Distally, in the pharyngeal arches, they give rise to the craniofacial skeleton and connective tissues. Fate choices are made as CNCC pattern into distinct destination compartments. In spite of this importance, the mechanism patterning CNCC is poorly defined. RESULTS: Here, we report that a novel ß-catenin-dependent regulation of N-Cadherin levels may drive CNCC patterning. In mouse embryos, at the first pharyngeal arch axial level, membrane ß-catenin levels correlate with the extent of N-cadherin-mediated adhesion and thus suggest the presence of collective and dispersed states of CNCC. Using in vitro human neural crest model and chemical modulators of ß-catenin levels, we show a requirement for down-modulating ß-catenin for regulating N-cadherin levels and cell-cell adhesion. Similarly, in ß-catenin gain-of-function mutant mouse embryos, CNCC fail to lower N-cadherin levels. This indicates a failure to reduce cell-cell adhesion, which may underlie the failure of mutant CNCC to populate first pharyngeal arch. CONCLUSION: We suggest that ß-catenin-mediated regulation of CNCC adhesion, a previously underappreciated mechanism, underlies the patterning of CNCC into fate-specific compartments.


Subject(s)
Body Patterning , Neural Crest/embryology , Pharynx/embryology , Skull/embryology , beta Catenin/metabolism , Animals , Mice , Mice, Transgenic , Neural Crest/cytology , Pharynx/cytology , Skull/cytology , beta Catenin/genetics
12.
Cell Death Dis ; 10(10): 736, 2019 09 30.
Article in English | MEDLINE | ID: mdl-31570693

ABSTRACT

Complex molecular cross talk between stromal cells and the leukemic cells in bone marrow is known to contribute significantly towards drug-resistance. Here, we have identified the molecular events that lead to stromal cells mediated therapy-resistance in acute myeloid leukemia (AML). Our work demonstrates that stromal cells downregulate miR-23a-5p levels in leukemic cells to protect them from the chemotherapy induced apoptosis. Downregulation of miR-23a-5p in leukemic cells leads to upregulation of protective autophagy by targeting TLR2 expression. Further, autophagy inhibitors when used as adjuvants along with conventional drugs can improve drug sensitivity in vitro as well in vivo in a mouse model of leukemia. Our work also demonstrates that this mechanism of bone marrow stromal cell mediated regulation of miR-23a-5p levels and subsequent molecular events are relevant predominantly in myeloid leukemia. Our results illustrate the critical and dynamic role of the bone marrow microenvironment in modulating miRNA expression in leukemic cells which could contribute significantly to drug resistance and subsequent relapse, possibly through persistence of minimal residual disease in this environment.


Subject(s)
Autophagy/genetics , Leukemia, Myeloid, Acute/genetics , MicroRNAs/genetics , Stromal Cells/drug effects , Toll-Like Receptor 2/drug effects , Animals , Apoptosis/drug effects , Autophagy/drug effects , Bone Marrow Cells/drug effects , Daunorubicin/pharmacology , Female , Gene Expression Regulation, Neoplastic/drug effects , Humans , Leukemia, Myeloid, Acute/drug therapy , Leukemia, Myeloid, Acute/pathology , Male , Mice , Neoplasm, Residual/genetics , Neoplasm, Residual/pathology , Stromal Cells/pathology , Tumor Microenvironment/drug effects , Xenograft Model Antitumor Assays
13.
FEBS Lett ; 593(22): 3198-3209, 2019 11.
Article in English | MEDLINE | ID: mdl-31529697

ABSTRACT

Planaria is an ideal system to study factors involved in regeneration and tissue homeostasis. Little is known about the role of metabolites and small molecules in stem cell maintenance and lineage specification in planarians. Using liquid chromatography and mass spectrometry (LC-MS)-based quantitative metabolomics, we determined the relative levels of metabolites in stem cells, progenitors, and differentiated cells of the planarian Schmidtea mediterranea. Tryptophan and its metabolic product serotonin are significantly enriched in stem cells and progenitor population. Serotonin biosynthesis in these cells is brought about by a noncanonical enzyme, phenylalanine hydroxylase. Knockdown of Smed-pah leads to complete disappearance of eyes in regenerating planaria, while exogenous supply of serotonin and its precursor rescues the eyeless phenotype. Our results demonstrate a key role for serotonin in eye regeneration.


Subject(s)
Metabolomics/methods , Planarians/physiology , Serotonin/metabolism , Animals , Cell Differentiation , Chromatography, Liquid , Mass Spectrometry , Ocular Physiological Phenomena , Phenylalanine Hydroxylase/metabolism , Regeneration , Stem Cells/cytology , Stem Cells/metabolism , Tryptophan/metabolism
14.
EMBO Rep ; 20(7): e47789, 2019 07.
Article in English | MEDLINE | ID: mdl-31267708

ABSTRACT

Transfer RNA (tRNA)-derived small RNAs (tsRNAs) have recently emerged as important regulators of protein translation and shown to have diverse biological functions. However, the underlying cellular and molecular mechanisms of tsRNA function in the context of dynamic cell-state transitions remain unclear. Expression analysis of tsRNAs in distinct heterologous cell and tissue models of stem vs. differentiated states revealed a differentiation-dependent enrichment of 5'-tsRNAs. We report the identification of a set of 5'-tsRNAs that is upregulated in differentiating mouse embryonic stem cells (mESCs). Notably, interactome studies with differentially enriched 5'-tsRNAs revealed a switch in their association with "effector" RNPs and "target" mRNAs in different cell states. We demonstrate that specific 5'-tsRNAs can preferentially interact with the RNA-binding protein, Igf2bp1, in the RA-induced differentiated state. This association influences the transcript stability and thereby translation of the pluripotency-promoting factor, c-Myc, thus providing a mechanistic basis for how 5'-tsRNAs can modulate stem cell states in mESCs. Together our study highlights the role of 5'-tsRNAs in defining distinct cell states.


Subject(s)
Cell Differentiation , MicroRNAs/metabolism , RNA, Transfer/metabolism , Animals , Cells, Cultured , HCT116 Cells , Humans , Mice , MicroRNAs/genetics , Mouse Embryonic Stem Cells/cytology , Mouse Embryonic Stem Cells/metabolism , Proto-Oncogene Proteins c-myc/genetics , Proto-Oncogene Proteins c-myc/metabolism , RNA Stability , RNA, Transfer/genetics , RNA-Binding Proteins/metabolism
15.
EMBO Rep ; 20(5)2019 05.
Article in English | MEDLINE | ID: mdl-30858340

ABSTRACT

Euchromatic histone methyltransferases (EHMTs), members of the KMT1 family, methylate histone and non-histone proteins. Here, we uncover a novel role for EHMTs in regulating heterochromatin anchorage to the nuclear periphery (NP) via non-histone methylation. We show that EHMTs methylate and stabilize LaminB1 (LMNB1), which associates with the H3K9me2-marked peripheral heterochromatin. Loss of LMNB1 methylation or EHMTs abrogates heterochromatin anchorage at the NP We further demonstrate that the loss of EHMTs induces many hallmarks of aging including global reduction of H3K27methyl marks and altered nuclear morphology. Consistent with this, we observe a gradual depletion of EHMTs, which correlates with loss of methylated LMNB1 and peripheral heterochromatin in aging human fibroblasts. Restoration of EHMT expression reverts peripheral heterochromatin defects in aged cells. Collectively, our work elucidates a new mechanism by which EHMTs regulate heterochromatin domain organization and reveals their impact on fundamental changes associated with the intrinsic aging process.


Subject(s)
Cell Nucleus/metabolism , Heterochromatin/metabolism , Histone-Lysine N-Methyltransferase/metabolism , Histones/metabolism , Lamin Type B/metabolism , Aging/metabolism , Cell Line , HEK293 Cells , Humans , Methylation
16.
Development ; 144(18): 3211-3223, 2017 09 15.
Article in English | MEDLINE | ID: mdl-28807895

ABSTRACT

Brain regeneration in planarians is mediated by precise spatiotemporal control of gene expression and is crucial for multiple aspects of neurogenesis. However, the mechanisms underpinning the gene regulation essential for brain regeneration are largely unknown. Here, we investigated the role of the miR-124 family of microRNAs in planarian brain regeneration. The miR-124 family (miR-124) is highly conserved in animals and regulates neurogenesis by facilitating neural differentiation, yet its role in neural wiring and brain organization is not known. We developed a novel method for delivering anti-miRs using liposomes for the functional knockdown of microRNAs. Smed-miR-124 knockdown revealed a key role for these microRNAs in neuronal organization during planarian brain regeneration. Our results also demonstrated an essential role for miR-124 in the generation of eye progenitors. Additionally, miR-124 regulates Smed-slit-1, which encodes an axon guidance protein, either by targeting slit-1 mRNA or, potentially, by modulating the canonical Notch pathway. Together, our results reveal a role for miR-124 in regulating the regeneration of a functional brain and visual system.


Subject(s)
Brain/physiology , MicroRNAs/metabolism , Planarians/genetics , Planarians/physiology , Regeneration , Visual Pathways/physiology , Animals , Biophysical Phenomena , Ganglia, Invertebrate/physiology , Gene Expression Regulation, Developmental , Gene Knockdown Techniques , Liposomes/chemistry , Membrane Fusion , MicroRNAs/genetics , Models, Biological , Neurons/metabolism , Penetrance , Phenotype , Receptors, Notch/metabolism , Reproducibility of Results , Signal Transduction , Viruses/metabolism
17.
Development ; 144(17): 3066-3079, 2017 09 01.
Article in English | MEDLINE | ID: mdl-28807897

ABSTRACT

Identifying key cellular events that facilitate stem cell function and tissue organization is crucial for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of regeneration, such as epithelialization and epidermal organization are critically regulated by a novel cytoplasmic poly A-binding protein, SMED-PABPC2. Knockdown of smed-pabpc2 leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing, resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling suggests that epidermal lineage transcripts, including zfp-1, are translationally regulated by SMED-PABPC2. Together, our results uncover a novel role for SMED-PABPC2 in the maintenance of epidermal and ECM integrity, critical for wound healing and subsequent processes for regeneration.


Subject(s)
Cytoplasm/metabolism , Epidermis/metabolism , Planarians/metabolism , Poly(A)-Binding Protein I/metabolism , Animals , Cell Lineage , Cell Proliferation , Epithelium/metabolism , Extracellular Matrix/metabolism , Gene Knockdown Techniques , Homeostasis , Models, Biological , Planarians/genetics , RNA, Messenger/genetics , RNA, Messenger/metabolism , Regeneration , Wound Healing
18.
G3 (Bethesda) ; 6(10): 3035-3048, 2016 10 13.
Article in English | MEDLINE | ID: mdl-27489207

ABSTRACT

In eukaryotes, 3' untranslated regions (UTRs) play important roles in regulating posttranscriptional gene expression. The 3'UTR is defined by regulated cleavage/polyadenylation of the pre-mRNA. The advent of next-generation sequencing technology has now enabled us to identify these events on a genome-wide scale. In this study, we used poly(A)-position profiling by sequencing (3P-Seq) to capture all poly(A) sites across the genome of the freshwater planarian, Schmidtea mediterranea, an ideal model system for exploring the process of regeneration and stem cell function. We identified the 3'UTRs for ∼14,000 transcripts and thus improved the existing gene annotations. We found 97 transcripts, which are polyadenylated within an internal exon, resulting in the shrinking of the ORF and loss of a predicted protein domain. Around 40% of the transcripts in planaria were alternatively polyadenylated (ApA), resulting either in an altered 3'UTR or a change in coding sequence. We identified specific ApA transcript isoforms that were subjected to miRNA mediated gene regulation using degradome sequencing. In this study, we also confirmed a tissue-specific expression pattern for alternate polyadenylated transcripts. The insights from this study highlight the potential role of ApA in regulating the gene expression essential for planarian regeneration.


Subject(s)
Genome, Helminth , Genome-Wide Association Study , Platyhelminths/genetics , Polyadenylation , RNA, Messenger/genetics , 3' Untranslated Regions , Animals , Computational Biology/methods , High-Throughput Nucleotide Sequencing , MicroRNAs/genetics , Molecular Sequence Annotation , Poly A , RNA Interference , RNA Processing, Post-Transcriptional , Reproducibility of Results
19.
Analyst ; 140(10): 3445-64, 2015 May 21.
Article in English | MEDLINE | ID: mdl-25815385

ABSTRACT

The fresh water planarian species Schmidtea mediterranea is an emerging stem cell model because of its capability to regenerate a whole animal from a small piece of tissue. It is one of the best model systems to address the basic mechanisms essential for regeneration. Here, we are interested in studying the roles of various amines, thiols and nucleotides in planarian regeneration, stem cell function and growth. We developed mass spectrometry based quantitative methods and validated the differential enrichment of 35 amines, 7 thiol metabolites and 4 nucleotides from both intact and regenerating planarians. Among the amines, alanine in sexual and asparagine in asexual are the highest (>1000 ng/mg) in the intact planarians. The levels of thiols such as cysteine and GSH are 651 and 1107 ng mg(-1) in planarians. Among the nucleotides, the level of cGMP is the lowest (0.03 ng mg(-1)) and the level of AMP is the highest (187 ng mg(-1)) in both of the planarian strains. We also noticed increasing levels of amines in both anterior and posterior regenerating planarians. The blastema from day 3 regenerating planarians also showed higher amounts of many amines. Interestingly, the thiol (cysteine and GSH) levels are well maintained during planarian regeneration. This suggests an inherent and effective mechanism to control induced oxidative stress because of the robust regeneration and stem cell proliferation. Like in intact planarians, the level of cGMP is also very low in regenerating planarians. Surprisingly, the levels of amines and thiols in head regenerating blastemas are ∼3 times higher compared to those for tail regenerating blastemas. Thus our results strongly indicate the potential roles of amines, thiols and nucleotides in planarian regeneration.


Subject(s)
Metabolomics/methods , Planarians/metabolism , Regeneration , Animals , Calibration , Chromatography, High Pressure Liquid , Limit of Detection , Metabolomics/standards , Planarians/cytology , Planarians/physiology , Reference Standards , Reproduction, Asexual , Species Specificity , Tandem Mass Spectrometry
20.
Sci Rep ; 4: 7357, 2014 Dec 08.
Article in English | MEDLINE | ID: mdl-25483805

ABSTRACT

Hedgehog (Hh) is a secreted morphogen that elicits differentiation and patterning in developing tissues. Multiple proposed mechanisms to regulate Hh dispersion includes lipoprotein particles and exosomes. Here we report that vertebrate Sonic Hedgehog (Shh) is secreted on two types of extracellular-vesicles/exosomes, from human cell lines and primary chick notochord cells. Although largely overlapping in size as estimated from electron micrographs, the two exosomal fractions exhibited distinct protein and RNA composition. We have probed the functional properties of these vesicles using cell-based assays of Hh-elicited gene expression. Our results suggest that while both Shh-containing exo-vesicular fractions can activate an ectopic Gli-luciferase construct, only exosomes co-expressing Integrins can activate endogenous Shh target genes HNF3ß and Olig2 during the differentiation of mouse ES cells to ventral neuronal progenitors. Taken together, our results demonstrate that primary vertebrate cells secrete Shh in distinct vesicular forms, and support a model where packaging of Shh along with other signaling proteins such as Integrins on exosomes modulates target gene activation. The existence of distinct classes of Shh-containing exosomes also suggests a previously unappreciated complexity for fine-tuning of Shh-mediated gradients and pattern formation.


Subject(s)
Exosomes/metabolism , Hedgehog Proteins/metabolism , Signal Transduction , Animals , Chick Embryo , Exosomes/genetics , Extracellular Space , HEK293 Cells , Humans , MicroRNAs/metabolism , Models, Biological , Protein Transport , Vertebrates
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