ABSTRACT
Multiple sclerosis (MS) is a demyelinating disease of inflammatory and autoimmune origin, which induces sensory and progressive motor impairments, including pain. Cells of the immune system actively participate in the pathogenesis and progression of MS by inducing neuroinflammation, tissue damage, and demyelination. Crotalphine (CRO), a structural analogue to a peptide firstly identified in Crotalus durissus terrificus snake venom, induces analgesia by endogenous opioid release and type 2 cannabinoid receptor (CB2) activation. Since CB2 activation downregulates neuroinflammation and ameliorates symptoms in mice models of MS, it was presently investigated whether CRO has a beneficial effect in the experimental autoimmune encephalomyelitis (EAE). CRO was administered on the 5th day after immunization, in a single dose, or five doses starting at the peak of disease. CRO partially reverted EAE-induced mechanical hyperalgesia and decreased the severity of the clinical signs. In addition, CRO decreases the inflammatory infiltrate and glial cells activation followed by TNF-α and IL-17 downregulation in the spinal cord. Peripherally, CRO recovers the EAE-induced impairment in myelin thickness in the sciatic nerve. Therefore, CRO interferes with central and peripheral neuroinflammation, opening perspectives to MS control.
Subject(s)
Encephalomyelitis, Autoimmune, Experimental/drug therapy , Neuroinflammatory Diseases/drug therapy , Pain/drug therapy , Peptides/pharmacology , Analgesics/pharmacology , Animals , Encephalomyelitis, Autoimmune, Experimental/physiopathology , Female , Hyperalgesia/drug therapy , Mice , Mice, Inbred C57BL , Multiple Sclerosis/drug therapy , Multiple Sclerosis/physiopathology , Receptor, Cannabinoid, CB2/drug effects , Receptor, Cannabinoid, CB2/metabolismABSTRACT
Multiple sclerosis (MS) is a demyelinating disease of inflammatory and autoimmune origin, which induces sensory and progressive motor impairments, including pain. Cells of the immune system actively participate in the pathogenesis and progression of MS by inducing neuroinflammation, tissue damage, and demyelination. Crotalphine (CRO), a structural analogue to a peptide firstly identified in Crotalus durissus terrificus snake venom, induces analgesia by endogenous opioid release and type 2 cannabinoid receptor (CB2) activation. Since CB2 activation downregulates neuroinflammation and ameliorates symptoms in mice models of MS, it was presently investigated whether CRO has a beneficial effect in the experimental autoimmune encephalomyelitis (EAE). CRO was administered on the 5th day after immunization, in a single dose, or five doses starting at the peak of disease. CRO partially reverted EAE-induced mechanical hyperalgesia and decreased the severity of the clinical signs. In addition, CRO decreases the inflammatory infiltrate and glial cells activation followed by TNF-α and IL-17 downregulation in the spinal cord. Peripherally, CRO recovers the EAE-induced impairment in myelin thickness in the sciatic nerve. Therefore, CRO interferes with central and peripheral neuroinflammation, opening perspectives to MS control.
ABSTRACT
We describe two patients with a confirmed diagnosis of high-grade gliomas (grades III/IV), both presenting with O6-methylguanine-DNA methyltransferase (MGMT) methylated and isocitrate dehydrogenase (IDH-1) mutated who, after subtotal resection, were submitted to chemoradiation and followed by PCV, a multiple drug regimen (procarbazine, lomustine, and vincristine) associated with cannabidiol (CBD). Both patients presented with satisfactory clinical and imaging responses at periodic evaluations. Immediately after chemoradiation therapy, one of the patients presented with an exacerbated and precocious pseudoprogression (PSD) assessed by magnetic resonance imaging (MRI), which was resolved in a short period. The other patient presented with a marked remission of altered areas compared with the post-operative scans as assessed by MRI. Such aspects are not commonly observed in patients only treated with conventional modalities. This observation might highlight the potential effect of CBD to increase PSD or improve chemoradiation responses that impact survival. Further investigation with more patients and critical molecular analyses should be performed.
ABSTRACT
BACKGROUND/AIMS: Previous studies have demonstrated that spinal cord injury (SCI) results in changes in sleep patterns through increased arousals and limb movements during sleep. Dramatic changes in muscle myosin heavy-chain isoforms have also been reported. The aim of this study was to investigate the characteristics of muscle fibers after SCI in rats with limb movements during sleep. METHODS: Forty male Wistar rats were divided into four groups: SHAM, SCI 3, 7 and 15 days. Animals were subjected to electrode insertion surgery, 24-hour baseline sleep recording, SCI, and subsequent sleep recording for 3, 7, or 15 consecutive days. In addition, the gastrocnemius muscle and spinal cord were collected for histopathological/histochemical analyses. RESULTS: Our results indicate a rapid and progressive decrease in the cross-sectional area of type I fibers in the gastrocnemius muscle (35.76-24.74 µm(2)) after SCI. Additionally, we found SCI-induced changes in sleep patterns. Following SCI, we also observed limb movements in sleeping rats, as well as significant negative moderate correlations between type I fibers and limb movement. CONCLUSION: Our study strengthened the hypothesis by correlation between changes in types of muscle fiber (decline in type I fibers) and an increase in limb movements during sleep after SCI.
Subject(s)
Movement Disorders/pathology , Muscle Fibers, Skeletal/pathology , Sleep Wake Disorders/pathology , Spinal Cord Injuries/pathology , Animals , Disease Models, Animal , Male , Movement Disorders/etiology , Rats , Rats, Wistar , Sleep Wake Disorders/etiology , Spinal Cord Injuries/complicationsSubject(s)
Adult , Female , Humans , Cerebellar Neoplasms/diagnosis , Ear Neoplasms/diagnosis , Hemangioma, Cavernous/diagnosis , Neurilemmoma/diagnosis , Cerebellopontine Angle , Cerebellar Neoplasms/surgery , Diagnosis, Differential , Hemangioma, Cavernous/surgery , Magnetic Resonance Imaging , Tomography, X-Ray ComputedSubject(s)
Cerebellar Neoplasms/diagnosis , Ear Neoplasms/diagnosis , Hemangioma, Cavernous/diagnosis , Neurilemmoma/diagnosis , Adult , Cerebellar Neoplasms/surgery , Cerebellopontine Angle , Diagnosis, Differential , Female , Hemangioma, Cavernous/surgery , Humans , Magnetic Resonance Imaging , Tomography, X-Ray ComputedABSTRACT
This study aims to evaluate the dimensions of the human brain, specifically in the frontal cortex, helping the analysis of neuroimaging. A form was made to register and describe encephalic measurements and 81 cerebral hemispheres (CH) were analyzed. Male individuals showed larger CH length; wider superior frontal gyrus in the right CH; bigger encephalic weight and corpus callosum (CC) width. The proportion of measurement from the frontal pole to the most anterior part of the CC genu, related to the CH length gets smaller with aging, whereas the average distance from the most posterior part of the splenum of the CC to the occipital pole was bigger in both male CHs and there was a tendency of decrease in this difference with aging.
Subject(s)
Brain/anatomy & histology , Cephalometry , Adult , Age Factors , Aged , Autopsy , Female , Frontal Lobe/anatomy & histology , Humans , Male , Middle Aged , Reference Values , Sex FactorsABSTRACT
Este estudo visa avaliar as dimensões do cérebro humano, particularmente do córtex frontal, podendo colaborar para as análises de neuroimagem. Foi elaborado um formulário para registro e descrição das medidas encefálicas. A amostra foi constituída por 81 hemisférios cerebrais (HC) adultos. Os homens apresentaram maior comprimento do HC; giro frontal superior mais largo no HC direito; maior peso encefálico e largura do corpo caloso (CC). A proporção da medida do pólo frontal à parte mais anterior do joelho do CC, em relação ao comprimento do HC diminui com o avanço da idade. Já a da média da distância da parte mais posterior do esplênio do CC ao pólo occipital foi maior em ambos HC dos homens e houve tendência à diminuição desta proporção com o avanço da idade.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Brain/anatomy & histology , Cephalometry , Age Factors , Autopsy , Frontal Lobe/anatomy & histology , Reference Values , Sex FactorsABSTRACT
A silicose é uma doença granulomatosa que acomete o pulmäo de pessoas expostas a cristais de sílica, porém näo encontramos na revisäo de literatura outro caso acometendo a mucosa nasal. Trata-se de uma paciente de 46 anos que começou a apresentar queda de dorso nasal em área K e rinorréia sanguinolenta, tendo evoluindo para perfuraçäo de septo; ao exame a mucosa apresentava aspecto granuloso de onde foi colhido material para exame anátomo-patológico que revelou cristais de sílica em meio a processo inflamatório linfoplasmocitário. Näo foi encontrada a forma de exposiçäo à sílica nessa paciente, que depois de um período de 3 anos sem sintomas, sem formaçäo de crostas nasais ou alteraçäo do aspecto externo da pirâmide nasal, foi submetida à rinoplastia para correçäo do afundamento em dorso nasal. Esse caso näo deve ser considerado apenas como curiosidade, mas como uma doença cuja patogenia näo está esclarecida
ABSTRACT
Objetivo: avaliar a correlaçäo entre o ultra-som de crânio seriado neonatal e achados anáyomo-patológicos em recém-nascidos pré-termo que evoluiram para óbito no período neonatal. Metodologia: foram estudados prospectivamente 22 pacientes com idade gestacional média de 30,5 semanas e peso médio de 1095 gramas no nascimento, nos quais foi realizado ultra-som de crânio de forma e a seriada e a análise cerebral macroscópica na autópsia, estabelecendo-se uma relaçäo entre o diagnóstico ultra-sonográfico e os achados anátomo-patológicos. Foi realizada a comparaçäo estatística entre estes dois fatores, por meio dos cálculos da sensibilidade, especificidade, valores preditivos positivo e negativo. Resultados: a especificidade do ultra-som foi de 88,9 por cento, a sensibilidade de 76,9 por cento, o valor preditivo positivo foi de 90,9 por cento e o valor preditivo negativo de 72,7 por cento
Subject(s)
Humans , Infant, Newborn , Autopsy , Infant, Premature , SkullABSTRACT
Relatou-se um caso de tumor maligno de nervo periférico (schwannoma) na órbita e revisou-se a literatura sobre essa rara lesäo