ABSTRACT
BACKGROUND: Craniomaxillofacial bone defects are currently reconstructed by using computer-aided design and manufacturing (CAD/CAM) processes. We have developed a novel digital medical support system that enables us to custom-make scaffolds to repair craniomaxillofacial bone defects using three-dimensional computed tomographic (CT) images and a rapid-prototyping method. METHODS: We created positive molds using CT data, CAD/CAM and a rapid prototyping method using 3D printing. Custom-made poly (glycolic acid) (PGA) and polymers poly (lactic acid) (PLA) scaffolds were prefabricated by a positive-negative mold interchange technique. A laser scanning system was used to evaluate the accuracy of the PGA/PLA scaffold. Bone marrow stem cells were incubated with the scaffold to assess biocompatibility. RESULTS: The mean error was <0.3 mm and confidence was >or=95% when the error was <1 mm. Results from in vitro cell culture demonstrated that the PGA/PLA scaffold had excellent cellular compatibility. CONCLUSIONS: This pilot study suggests that custom-made PGA/PLA scaffolds infiltrated with bone marrow stem cells may be effective for future treatment of craniomaxillofacial bone injuries.
Subject(s)
Bone Regeneration , Imaging, Three-Dimensional/methods , Lactic Acid , Mandible/surgery , Polyglycolic Acid , Polymers , Tissue Scaffolds , Animals , Craniofacial Abnormalities/surgery , Dogs , Extracellular Matrix/ultrastructure , In Vitro Techniques , Lasers , Male , Mandible/diagnostic imaging , Mandible/ultrastructure , Materials Testing , Microscopy, Electron, Scanning , Models, Anatomic , Orthopedic Procedures/instrumentation , Orthopedic Procedures/methods , Pilot Projects , Polyesters , Plastic Surgery Procedures/instrumentation , Plastic Surgery Procedures/methods , Tomography, X-Ray ComputedABSTRACT
PURPOSE: This study evaluated the influence of bonding strength of heat-polymerized denture soft reline resin by 4 different surface treatments bonded to denture base resin. METHODS: Denture base resin (8mm in diameter and 15 mm in length) were fabricated from heat-polymerized acrylic resin (Densply). And heat-polymerized denture soft reline resin disks (Physio liner) were also made (4 mm thick and 10 mm diameter). Two denture base resins were bonded to the denture reline resin disk at the centure on the two sides of the disk. All the specimen were divided into 4 groups (n=10) with 3 different surface treatments: (1) no treatment(control), (2) sandblasted, (3) surface liner application, (4) surface liner application+sandblasted. Bond strength (MPa) was measured by tension bond testing at a 12.7 mm/min speed until the soft reline resin debonded from the base resin. Data were statistically analyzed by SPSS 10.0 software package for ANOVA. RESULTS: The mean bond strengths of group 1, 2, 3, 4 were 3.518, 2.834, 4.077, 3.852MPa respectively. The bonding strength of all the three groups showed significant difference (P<0.0014). The group with surface liner application showed significantly highest bonding strength, while the group with sandblasted showed the lowest bonding strength. CONCLUSIONS: The use of surface liner can significantly improve the bonding strength of heat-polymerized denture soft reline resin to the denture base resin, while the bonding strength decreased after using sandblasting.