Physiological and biochemical roles of ascorbic acid on mitigation of abiotic stresses in plants.

Under conditions of abiotic stress several physiological and biochemical processes in plants can be modified. The production of reactive oxygen species (ROS) is toxic at high concentrations and promotes RNA, DNA and plant cell membrane degradation. Plants have enzymatic and non-enzymatic adaptation mechanisms to act against ROS detoxification. Ascorbic acid (AsA) is the non-enzymatic compound essential for several biological functions, which acts in the elimination and balance of ROS production and with the potential to promote several physiological functions in plants, such as the photosynthetic process. For plant development, AsA plays an important role in cell division, osmotic adjustment, hormone biosynthesis, and as an enzymatic cofactor. In this review, the redox reactions, biosynthetic pathways, and the physiological and biochemical functions of AsA against abiotic stress in plants are discussed. The concentration of AsA in plants can vary between species and depend on the biosynthetic pathways d-mannose/l-galactose, d-galacturonate, euglenids, and d-glucuronate. Although the endogenous levels of AsA in plants are used in large amounts in cell metabolism, the exogenous application of AsA further increases these endogenous levels to promote the antioxidant system and ameliorate the effects produced by abiotic stress. Foliar application of AsA promotes antioxidant metabolism in plants subjected to climate change conditions, also allowing the production of foods with higher nutritional quality and food safety, given the fact that AsA is biologically essential in the human diet.

Roles of selenium in mineral plant nutrition: ROS scavenging responses against abiotic stresses.

Agronomic biofortification of crops with selenium (Se) is an important strategy to minimize hidden hunger and increase nutrient intake in poor populations. Selenium is an element that has several physiological and biochemical characteristics, such as the mitigation of different types of abiotic stress. Selenoproteins act as powerful antioxidants in plant metabolism through the glutathione peroxidase (GSH) pathway, and provide an increased activity for enzymatic (SOD, CAT, and APX) and non-enzymatic (ascorbic acid, flavonoids, and tocopherols) compounds that act in reactive oxygen species (ROS) scavenging system and cell detoxification. Selenium helps to inhibit the damage caused by climate changes such as drought, salinity, heavy metals, and extreme temperature. Also, Se regulates antenna complex of photosynthesis, protecting chlorophylls by raising photosynthetic pigments. However, Se concentrations in soils vary widely in the earth's crust. Soil Se availability regulates the uptake, transport, accumulation, and speciation in plants. Foliar Se application at the concentration 50 g ha-1 applied as sodium selenate increases the antioxidant, photosynthetic metabolism, and yield of several crops. Foliar Se application is a strategy to minimize soil adsorption and root accumulation. However, the limit between the beneficial and toxic effects of Se requires research to establish an optimal dose for each plant species under different edaphoclimatic conditions. In this review, we present the compilation of several studies on agronomic biofortification of plants with Se to ensure food production and food security to mitigate hidden hunger and improve the health of the population.

Assessment of selenium spatial distribution using µ-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responses.

Low concentrations of selenium (Se) are beneficial for plant growth. Foliar Se application at high concentrations is toxic to plants due to the formation of reactive oxygen species (ROS). This study characterized Se toxicity symptoms using X-ray fluorescence (XRF) technique in response to foliar Se application in cowpea plants. Five Se concentrations (0, 10, 25, 50, 100 e 150 g ha-1) were sprayed on leaves as sodium selenate. The visual symptoms of Se toxicity in cowpea leaves were separated into two stages: I) necrotic points with an irregular distribution and internerval chlorosis at the leaf limb border (50-100 g ha-1); II) total chlorosis with the formation of dark brown necrotic lesions (150 g ha-1). Foliar Se application at 50 g ha-1 increased photosynthetic pigments and yield. Ultrastructural analyses showed that Se foliar application above 50 g ha-1 disarranged the upper epidermis of cowpea leaves. Furthermore, Se application above 100 g ha-1 significantly increased the hydrogen peroxide concentration and lipid peroxidation inducing necrotic leaf lesions. Mapping of the elements in leaves using the XRF revealed high Se intensity, specifically in leaf necrotic lesions accompanied by calcium (Ca) as a possible attenuating mechanism of plant stress. The distribution of Se intensities in the seeds was homogeneous, without specific accumulation sites. Phosphorus (P) and sulfur (S) were found primarily located in the embryonic region. Understanding the factors involved in Se accumulation and its interaction with Ca support new preventive measurement technologies to prevent Se toxicity in plants.