Simple and rapid detection of bisphenol A using a gold nanoparticle-based colorimetric aptasensor.

A colorimetric aptasensor was developed for the simple and rapid detection of bisphenol A (BPA). The aptasensor was designed to consist of colloidal gold nanoparticles (AuNPs) and a BPA-specific 24-bp aptamer. The AuNP-aptamer conjugates underwent an electrolyte-induced aggregation in the presence of sub-ppb levels of BPA. The surface plasmon resonance shift of AuNPs facilitated a color change from red to blue upon aggregation, which was visually observed by the naked eye. The corresponding visual limit of detection of BPA was as low as 1 pg/mL (0.004 nM). The aptasensor also achieved a selective detection of BPA over a variety of BPA analogs. The applicability of the aptasensor was verified via a successful detection of BPA in a single grain of rice. This result indicates that the colorimetric aptasensor can be used in a screening procedure for food and environmental monitoring, with reliable performance to sub-ppb levels of BPA detection.

Hydrodynamic Layer-by-Layer Assembly of Transferable Enzymatic Conductive Nanonetworks for Enzyme-Sticker-Based Contact Printing of Electrochemical Biosensors.

Realizing high-performance electrochemical biosensors in a simple contact-printing-based approach significantly increases the applicability of integrated flexible biosensors. Herein, an enzyme-sticker-based approach that enables flexible and multielectrochemical sensors via simple contact-transfer printing is reported. The enzyme sticker consists of an enzymatic conductive network film and a polymeric support. The enzyme-incorporated nanostructured conductive network showing an efficient electrical coupling was assembled via the hydrodynamic layer-by-layer assembly of redox enzymes, polyelectrolytes, single-walled carbon nanotubes, and a biological glue material, M13 phage. The enzymatic conductive network on a polymeric membrane support was facilely wet contact-transfer printed onto integrated electrode systems by exploiting varying degrees of hydrophilicity displayed by the enzymatic electronic film, polymeric support, and receiving electrodes of the sensor system. The glucose sensors fabricated using the enzyme sticker detected glucose at a concentration of as low as 35 µM and showed high selectivity and stability. Furthermore, a flexible dual-sensor array capable of detecting both glucose and lactate was demonstrated using the versatile enzyme sticker concept. This work presents a new route toward assembling and integrating hybrid nanomaterials with efficient electrochemical coupling for high-performance biosensors and health-monitoring devices as well as for emerging bioelectronics and electrochemical devices.