The clinical effect of sodium hypochlorite oral rinse on peri-implantitis lesion: A pilot study.

Peri-implantitis poses an imminent challenge to the field of implant dentistry. Considering the promising findings of sodium hypochlorite and periodontal lesions, the aim of the present study was to evaluate the clinical effects of sodium hypochlorite oral rinse on peri-implantitis lesions. Twelve peri-implantitis patients were instructed to rinse with 15 mL of a fresh solution of 0.25% sodium hypochlorite for 30 s twice a week for 3 months. At baseline and 3-month visits, probing depth and modified sulcular bleeding index were recorded at 6 points per lesion (mesiobuccal, buccal, distobuccal, mesiolingual, lingual, and distolingual). Individual and total bacterial loads of 18 pre-designated species of microorganisms were analyzed by real-time PCR methods. Probing depth decreased after the experiment, with an average difference of 1.1 mm and a standard deviation of 1.7 mm. The modified sulcular bleeding index decreased by a mean value of 0.8 with a standard deviation of 1.1. This study demonstrated the clinical effects of sodium hypochlorite oral rinse on peri-implantitis lesions and the reduction of periodontal probing depth and gingival bleeding index. This study suggested that the concentration of 0.25% be used for treatment of peri-implantitis.

Quantitative Analysis of the Head Tilt Using Three-Dimensional Temporal Scan in Children with Torticollis.

The head tilt of patients with torticollis is usually evaluated subjectively in clinical practice and measuring it in young children is very limited due to poor cooperation. No study has yet evaluated the head tilt using a three-dimensional (3D) scan and compared it with other measurement methods. Therefore, this study aimed to objectively demonstrate head tilt through clinical measurements and a 3D scan in children with torticollis. A total of 52 children (30 males, 22 females; age 4.6 ± 3.2 years) diagnosed with torticollis and 52 adults (26 men, 26 women; age 34.42 ± 10.4 years) without torticollis participated in this study. The clinical measurements were performed using a goniometer and still photography methods. Additionally, the head tilt was analyzed using a 3D scanner (3dMD scan, 3dMD Inc., Atlanta, GA, USA). There was a high correlation between the other methods and 3D angles, and the cut-off value of the 3D angles for the diagnosis of torticollis was also presented. The area under the curve of the 3D angle was 0.872, which was confirmed by a moderately accurate test and showed a strong correlation compared with other conventional tests. Therefore, we suggest that measuring the degree of torticollis three-dimensionally is significant.

Development of a human-friendly visual inspection method for painted vehicle bodies.

Visual inspections performed in the final stage of the vehicle manufacturing process are crucial for assuring the quality of painted vehicle bodies. However, lengthy visual inspections can cause fatigue and discomfort of the eyes, which may adversely affect inspection accuracy and efficiency. This study developed a new human-friendly visual inspection method for the detection of defects (e.g., scar and dent) on vehicle bodies, and compared its performance to a conventional inspection method in terms of critical fusion frequency (Hz, indirect measure of eye fatigue), defect detectability (%), and subjective satisfaction score (7-point Likert scale). The new method was devised to project bright-dark linear stripes onto the surface of the vehicle body and created emergent features (distorted stripes) where a defect existed. The critical fusion frequency of the new method decreased slightly (3.7%) after a 30-minute visual inspection task, whereas that of the conventional method dropped substantially (11.0%), which implied more severe eye fatigue. Additionally, the new method had significantly higher defect detectability (92.1%) and satisfaction score (5.8 points) than those (73.4% and 3.5 points) of the conventional method.

Method for improving the speed and pattern quality of a DMD maskless lithography system using a pulse exposure method.

Maskless lithography based on a digital micromirror device (DMD) has the advantages of high process flexibility and a low production cost. However, due to the trade-off relationship between the pixel size and exposure area, it is challenging to achieve high resolutions and high patterning speeds at the same time, which hinders the wider application of this technology in micro- and nano-fabrication processes. In addition, micromirrors in DMDs create pixelated edges that limit the pattern quality. In this paper, we propose a novel DMD maskless lithography method to improve the pattern quality during high-speed continuous patterning by means of pulse exposure and oblique scanning processes. A unique criterion, the pixel occupancy, was devised to determine the parameters related to the pulse exposure and oblique scanning optimally. We also studied how the duty cycle of the pulse exposure affects the pattern quality. As a result, we were able to increase the scanning speed up to the speed limit considering the damage threshold of the DMD and improve the pattern quality by resolving the pixelation problem. We anticipate that this method can be used in various microfabrication fields with short product life cycles or in those that require custom designs, such as the manufacturing of PCBs, MEMS devices, and micro-optics devices, among others.

Vibroacoustic analysis of dental air turbine noise.

OBJECTIVE: The objective of this study is to analyze the noise mechanism of dental air turbine handpiece with vibroacoustic simulation. MATERIALS AND METHODS: The operational part of the Gentle Silence Lux 8000B (KaVo Dental GmbH) was disassembled and scanned. The scanned data were rendered to smooth irregularities and then virtually reassembled. The rendering was 3D mesh modeled for the analysis. And, the interior void space and exterior space was mesh modeled as air layer. As per simulation input informations, the material property of steel was provided for the handpiece components. Supplied air pressure of 0.22 MPa at the inlet and static temperature of 25 °C was provided as operating conditions. Twenty virtual microphones were arrayed to measure the noise. Vibroacoustic noise simulation was performed with ACTRAN 2021 (MSC software corporation). RESULTS: The mean value of noise ranged from 49.88 to 66.38 dB while the peak value ranged from 69.53 to 81.64 dB depending on the microphone position. All microphones showed the similar noise pattern which had peak amplitude at around 4500 Hz. The calculated natural frequency of interior air layer was 4478.92 Hz and 7573.77 Hz. DISCUSSION: The simulated result showed similar tonal noise of dental handpiece suggesting air resonance phenomenon as a possible cause of dental handpiece noise. CONCLUSION: Vibroacoustic analysis of the air layer contained within the dental air turbine handpiece showed the resonance peak noise at 4478.92 Hz under the simulated conditions.

A framework for effective face-mask contact modeling based on finite element analysis for custom design of a facial mask.

A novel contact model is presented to efficiently solve a face-mask contact problem by using the finite element (FE) method for the optimized design of a custom facial mask. Simulation of contact pressure for various mask designs considering material properties of the face allows virtual evaluation of the suitability of a mask design for a person's face without conducting empirical measurement of the face-mask contact pressure. The proposed contact model is accomplished by combining three approaches to reduce the calculation cost of simulating the face-mask contact: (1) use of a simplified and modifiable mask model that applies a spline curve to design points; (2) reduction of the FE model of the face by applying static condensation; and (3) application of a contact assumption that uses the Lagrange multiplier method. A numerical case study of a medical mask design showed that the proposed model could calculate the face-mask contact pressure efficiently (0.0448 sec per design). In a pilot usability experiment, the measured contact pressure was found similar values (range of mean contact pressure: 0.0093 ~ 0.0150 MPa) to the estimated values (range of mean contact pressure: 0.0097 ~ 0.0116 MPa).

Head and facial dimensions of Chilean workers for design purposes and the differences with other populations.

BACKGROUND: An appropriate match between a product and its end-users requires anthropometric data, which show variations among different countries. Proper Personal Protection Equipment (PPE) fit is key for safety and comfort. Chile had no head and facial anthropometric data available in order to design face/head PPE. OBJECTIVE: To describe face/head anthropometric characteristics of Chilean workers. Additionally, this study compared those dimensions against other populations (United States (US), South Korea and China). METHODS: An anthropometric survey involving 21 measures was conducted between September 2013 to May 2016 using stratified sampling. The measurements were based on ISO/TS 16976-2 and ISO 15535 to ensure the highest standards possible, and a total of 474 workers, aged from 18 to 66 years old, participated in the survey. RESULTS: The biggest differences were in Neck circumference, Weight, Nose breadth, Nose protrusion, Bitragion chin arc, Face length, Subnasale-sellion length, Face width, Bigonial breadth and Bitragion subnasal arc. Head length of Chileans were longer than Chinese and South Korean ones, but shorter than US Head length. Chilean Head breadth is smaller than Chinese and South Korean ones. Chileans Face length was the largest observed. Face width of Chileans was smaller than US and Chinese ones. Gender specific differences were also observed in the inter-country comparisons. Chilean males had larger anthropometric dimensions than females. CONCLUSION: Chilean Head and face dimensions differed significantly when compared against US, South Korean and Chines Head and Face dimensions. Chileans have longer and narrower faces with wider mouths, with a head size in between the US and Asian heads. Face and head PPE for Chileans should use dimensions in the current paper in order to ensure proper fit.

Ergonomics and Personalization of Noninvasive Ventilation Masks.

Although noninvasive ventilation (NIV) is administered to manage respiratory failure due to various causes, safety and effectiveness issues associated with the use of NIV masks have been reported. The present article aims to provide health professionals with a comprehensive review of the ergonomic considerations of NIV masks in terms of design, evaluation, and personalization. Based on a review of 93 papers, we provide guidelines for mask selection and troubleshooting during mask use as well as ergonomic approaches including face anthropometry, sizing systems, mask design, evaluation, and personalization. The comprehensive information presented in this review provides ergonomic perspectives to identify and prevent safety and usability problems associated with the use of NIV masks. Ergonomic improvement and personalization are important goals in order to facilitate the success of NIV treatment.

Determination of the reliability and repeatability of a quantitative occlusal analyzer by using a piezoelectric film sensor: An in vitro study.

STATEMENT OF PROBLEM: A digital quantitative occlusal analyzer with claimed lower cost and easier maneuverability has been introduced to replace conventional methods. However, information regarding its performance is scarce. PURPOSE: The purpose of this in vitro study was to evaluate the reliability and repeatability of a newly introduced digital occlusal analysis device (Accura) and to compare it with an established occlusal analyzer (T-scan Novus). MATERIAL AND METHODS: The sensor films of both devices were positioned between titanium maxillary and mandibular models that were equilibrated and arbitrarily mounted on a semiadjustable articulator. Compressive force was applied to the upper arm of the articulator with a universal testing machine, increased gradually to 50 N, and then released automatically. The time and force measurements from the universal testing machine and the tested devices were recorded by operating software programs. Five articulating film sensors of each device were tested 3 times each by 2 examiners and were repeated on consecutive days, totaling 60 trials per device. Reliability was defined as an agreement between the loaded force and measured force. Repeatability was defined as an agreement between repeated measurements of the Accura and T-scan Novus. Intraclass correlation coefficient was calculated for the statistical analysis (α=.05). RESULTS: The calculated intraclass correlation coefficient of the Accura and the universal testing machine was 0.952. The intraclass correlation coefficient of the T-scan Novus and the universal testing machine was 0.963. Intraclass correlation coefficients that were calculated to compare the data acquired from different days were 0.938 for the Accura and 0.911 for the T-scan Novus. CONCLUSIONS: The experimental results indicated that the reliability of both the Accura and T-scan Novus was excellent for measuring occlusal forces. In addition, both the Accura and T-scan Novus exhibited excellent repeatability.

Doxorubicin-Resistant TNBC Cells Exhibit Rapid Growth with Cancer Stem Cell-like Properties and EMT Phenotype, Which Can Be Transferred to Parental Cells through Autocrine Signaling.

Emerging evidence suggests that breast cancer stem cells (BCSCs), and epithelial-mesenchymal transition (EMT) may be involved in resistance to doxorubicin. However, it is unlear whether the doxorubicin-induced EMT and expansion of BCSCs is related to cancer dormancy, or outgrowing cancer cells with maintaining resistance to doxorubicin, or whether the phenotypes can be transferred to other doxorubicin-sensitive cells. Here, we characterized the phenotype of doxorubicin-resistant TNBC cells while monitoring the EMT process and expansion of CSCs during the establishment of doxorubicin-resistant MDA-MB-231 human breast cancer cells (DRM cells). In addition, we assessed the potential signaling associated with the EMT process and expansion of CSCs in doxorubicin-resistance of DRM cells. DRM cells exhibited morphological changes from spindle-shaped MDA-MB-231 cells into round-shaped giant cells. They exhibited highly proliferative, EMT, adhesive, and invasive phenotypes. Molecularly, they showed up-regulation of Cyclin D1, mesenchymal markers (ß-catenin, and N-cadherin), MMP-2, MMP-9, ICAM-1 and down-regulation of E-cadherin. As the molecular mechanisms responsible for the resistance to doxorubicin, up-regulation of EGFR and its downstream signaling, were suggested. AKT and ERK1/2 expression were also increased in DRM cells with the advancement of resistance to doxorubicin. Furthermore, doxorubicin resistance of DRM cells can be transferred by autocrine signaling. In conclusion, DRM cells harbored EMT features with CSC properties possessing increased proliferation, invasion, migration, and adhesion ability. The doxorubicin resistance, and doxorubicin-induced EMT and CSC properties of DRM cells, can be transferred to parental cells through autocrine signaling. Lastly, this feature of DRM cells might be associated with the up-regulation of EGFR.

Development of an ergonomic design process for smartphone hard key locations.

Smartphone hard key locations need to be ergonomically determined to improve grip stability and operational efficiency for users' convenience. The present study proposed an ergonomic design process that determines smartphone hard key locations by statistically analyzing the preferred hard key control areas of users with various hand sizes based on users' preferred grip postures and hard key control areas. The proposed design process analyzes the characteristics of product design, user, task, and use context, the types of preferred grip posture, the preference distribution of grip posture, and the preference distribution of hard-key area, and then recommends the locations of hard keys by considering the preference distribution of hard-key area and design constraints. The proposed design process was applied to a smartphone with a 5-inch screen, resulting in 77-96 mm from the bottom of the device for a volume key to 20 mm on the left side and 88-97 mm for a power key to 10 mm on the right side. The proposed design process for the determination of smartphone hard-key locations would be of use to determine the locations of various portable product interfaces.

Effects of abutment screw preload in two implant connection systems: A 3D finite element study.

STATEMENT OF PROBLEM: Finite element analysis (FEA) has been used to evaluate the biomechanical behaviors of dental implants. However, in some FEA studies, the influence of the preload condition has been omitted to simplify the analysis. This might affect the results of biomechanical analysis significantly. The preload condition requires analysis. PURPOSE: The purpose of this FEA study was to evaluate and verify the effects of the presence of the preload condition on abutment screws under the occlusal load for external and internal hexagonal connection systems. MATERIAL AND METHODS: The finite element models consisting of bone blocks, 2 different implant systems (Osstem US and GS system; Osstem Implant Co), and crowns were created. With these components, a total of 6 models with different conditions were constructed for FEA: external hexagonal connection system only with preload (EO), external hexagonal connection system with no preload but occlusal load (EN), external hexagonal system with both preload and occlusal load (EP), internal hexagonal system only with preload (IO), internal hexagonal system with no preload but occlusal load (IN), and internal hexagonal system with both preload and occlusal load (IP). An 11.3-degree oblique load (100 N) to the axis of the implant was applied on the occlusal surface of the crown for the models with occlusal load. A preload of 825 N was applied in the abutment screw of the models EO, EP, IO, and IP. The maximum von Mises stress, maximum principal stress, and maximum displacement of the components of the models were evaluated. RESULTS: Both external and internal connection systems resulted in higher maximum von Mises stress and maximum principal stress values in the presence of preload in the abutment screw. The internal connection system showed higher displacement values than the external system with or without occlusal loading, and values tended to increase with the preload condition. CONCLUSIONS: The presence of a preload condition significantly affected the biomechanical behaviors of the components of 2 different connection systems. The preload condition should be included in FEA to achieve more realistic results.

Analysis of natural finger-press motions for design of trackball buttons.

This study analysed natural press motions of the index, middle and ring fingers for ergonomic design of the positions and surface angles of the left, middle and right trackball buttons. Finger motions of 26 male participants for naturally pressing the trackball buttons were recorded after the participants adjusted the trackball buttons to their preferred locations for comfortable pressing. The natural positions of the finger pulps formed a symmetrically rainbow-shaped reach zone for the fingers. The natural press angles of the fingers' motion trajectories to the vertical reference line ranged from 14.2° to 20.5°, suggesting an 18-degree surface from the horizontal line for the trackball buttons. Regression formulas (adjusted R2 = 0.90 ± 0.07 and mean squared error = 8.55 ± 7.52 mm) were established to estimate the natural positions of finger pulps from hand segment lengths and joint angles for a population having different hand sizes from this study. Relevance to industry.

Accuracy of new implant impression technique using dual arch tray and bite impression coping.

PURPOSE: The purpose of this in vitro study was to evaluate the accuracy of a new implant impression technique using bite impression coping and a dual arch tray. MATERIALS AND METHODS: Two implant fixtures were placed on maxillary left second premolar and first molar area in dentoform model. The model with two fixtures was used as the reference. The impression was divided into 2 groups, n=10 each. In group 1, heavy/light body silicone impression was made with pick up impression copings and open tray. In group 2, putty/light body silicone impression was made with bite impression copings and dual arch tray. The reference model and the master casts with implant scan bodies were scanned by a laboratory scanner. Surface tessellation language (STL) datasets from test groups was superimposed with STL dataset of reference model using inspection software. The three-dimensional deviation between the reference model and impression models was calculated and illustrated as a color-map. Data was analyzed by independent samples T-test of variance at α=.05. RESULTS: The mean 3D implant deviations of pick up impression group (group 1) and dual arch impression group (group 2) were 0.029 mm and 0.034 mm, respectively. The difference in 3D deviations between groups 1 and 2 was not statistically significant (P=.075). CONCLUSION: Within limitations of this study, the accuracy of implant impression using a bite impression coping and dual arch tray is comparable to that of conventional pick-up impression.

Anthropometric analysis of 3D ear scans of Koreans and Caucasians for ear product design.

The present study measured 25 dimensions of the ear including the concha and ear canal for ergonomic design of ear products and compared with existing ear measurement studies. Scanning and casting methods were employed to produce 3D ear images for 230 Koreans and 96 Caucasians and measurements of the ear dimensions were obtained by identifying 21 landmarks on individual ear scan image. The Korean ear measurements were found significantly larger (mean difference d¯ = 0.4-3.7 mm) and more varied (ratio of SDs =1.01-1.55) than those of Caucasians in most of ear dimensions. The average ear length and ear breadth of male were significantly longer ( d¯ = 1.3-7.0 mm) and wider ( d¯ = 0.8-3.0 mm) than those of female. Use of gender- and ethnicity-composite ear data is recommended in product design due to the much larger intra-population variations (7.5-22.2 mm) than the corresponding inter-population variations. Practitioner Summary: The 3D ear measurements of Koreans and Caucasians were collected and compared with those of different ethnic populations. The distinct ear features of the populations identified in this study are applicable to ergonomic design of ear products with better fit and comfort. Abbreviations: CCW: cavum concha width; CV: coefficient of variation; EB: ear breadth; EL: ear length; SD: standard deviation; SE: sampling error; 3D: 3 dimensional.

Investigation on improvement of lateral resolution of continuous wave STED microscopy by standing wave illumination.

In this paper, we report the enhancement of resolution of continuous wave (CW) stimulated emission depletion (STED) microscopy by a novel method of structured illumination of an excitation beam. Illumination by multiple excitation beams through the specific pupil apertures with high in-plane wave vectors leads to interference of diffracted light flux near the focal plane, resulting in the contraction of the point spread function (PSF) of the excitation. Light spot reduction by the suggested standing wave (SW) illumination method contributes to make up much lower depletion efficiency of the CW STED microscopy than that of the pulsed STED method. First, theoretical analysis showed that the full width at half maximum (FWHM) of the effective PSF on the detection plane is expected to be smaller than 25% of that of conventional CW STED. Second, through the simulation, it was elucidated that both the donut-shaped PSF of the depletion beam and the confocal optics suppress undesired contribution of sidelobes of the PSF by the SW illumination to the effective PSF of the STED system. Finally, through the imaging experiment on 40-nm fluorescent beads with the developed SW-CW STED microscopy system, we obtained the result which follows the overall tendency from the simulation in the aspects of resolution improvement and reduction of sidelobes. Based on the obtained result, we expect that the proposed method can become one of the strategies to enhance the resolution of the CW STED microscopy.

Ergonomic evaluation of pilot oxygen mask designs.

A revised pilot oxygen mask design was developed for better fit to the Korean Air Force pilots' faces. The present study compared an existing pilot oxygen mask and a prototype of the revised mask design with 88 Korean Air Force pilots in terms of subjective discomfort, facial contact pressure, and slip distance on the face in high gravity. The average discomfort levels, facial contact pressures, and slip distance of the revised mask were reduced by 33%-56%, 11%-33%, and 24%, respectively, compared to those of the existing oxygen mask. The mask evaluation method employed in the study can be applied to ergonomic evaluation of full- or half-face mask designs.

Material interface detection based on secondary electron images for focused ion beam machining.

A method for interface detection is proposed for focused ion beam (FIB) processes of multilayered targets. As multilayers have emerged as promising structures for nanodevices, the FIB machining of multilayers has become a challenging issue. We proposed material interface detection by monitoring secondary electron (SE) images captured during the FIB process. The average of the gray-levels and the skewness coefficient of gray-level histograms of the SE images were evaluated to recognize endpoints for the FIB processes. The FIB process control with the proposed method was demonstrated by fabricating the nanostructures on the multilayered target without thickness information. It was also demonstrated on a curved surface. Grooves with a desired depth into the target and an aperture as an opening window were precisely fabricated by the FIB process control. The proposed strategy of the FIB process can be used for complex substrates such as curved or flexible targets.

Vertical repositioning accuracy of magnetic mounting systems on 4 articulator models.

STATEMENT OF PROBLEM: Research of the ability of a cast mounted on an articulator on maintaining the identical position of a cast mounted on an articulator after repeated repositioning is lacking, despite the possible effects this may have on the occlusion of a mounted cast. PURPOSE: The purpose of this in vitro study was to verify and compare the vertical repositioning accuracy of 4 different, commercially available articulator magnetic mounting plate systems. MATERIAL AND METHODS: Four articulators and their associated magnetic mounting plates were selected for the study. These were the Artex AR articulator (Amann Girrbach AG), the Denar Mark II articulator (Whip Mix Corp), the Kavo Protar Evo articulator (Kavo Dental GmbH), and the SAM3 articulator (SAM Präzisionstechnik GmbH). Three new magnetic mounting plates were prepared for each articulator system. The repositioning accuracy of each mounting plate was evaluated by comparing the standard deviation of the vertical distances measured between the mounting plate and a laser displacement sensor. The lower arm of the articulator was secured, and the vertical distance was measured by positioning the laser displacement sensor positioned vertically above the mounting plate. Once the vertical distance was measured, the mounting plate was detached from the articulator and reattached manually to prepare for the next measurement. This procedure was repeated 30 times for each of the 3 magnetic mounting plates. Data were analyzed by ANOVA for 2-stage nested design and the Levene test (α=.05). RESULTS: Significant differences were detected among articulator systems and between magnetic mounting plates of the same type. The standard deviations of the measurements made with the Artex AR articulator, Denar Mark II articulator, Kavo Protar Evo articulator, and SAM3 articulator were 0.0027, 0.0308, 0.0214, and 0.0215 mm, respectively. Thus, the repositioning accuracy could be ranked in the order as follows: Artex AR, Kavo Protar Evo, SAM3, and Denar Mark II. CONCLUSIONS: The position of the magnetic mounting plate after repositioning did not maintain an identical position in the vertical dimension on any of the 4 articulator models tested. The repositioning accuracy of the mounting plates showed significant differences among the articulators tested in this study.