ABSTRACT
Background: Bovine mastitis, a global disease that is responsible for large economic losses each year due to lower milkyield and reduced milk quality. In some countries, especially in China, Streptococcus agalactiae has become one of themost frequently detected pathogen. Antibiotic treatment and vaccine immunization are important strategies for the controlof infectious diseases. The main objective of the present study was to evaluate distribution of bovine mastitis pathogensand antimicrobial resistance of S. agalactiae, and contribute to the treatment of bovine mastitis.Materials, Methods & Results: Clinical mastitis samples (n= 1,122) were collected from 27 dairy farms located in 15different provinces of China during 2012-2018. The pathogens were identified by 16S rDNA method. Antimicrobial susceptibility was assessed by disc diffusion method. Molecular characteristics was distinguished based on PCR. The resultsshowed that the main pathogens were Streptococcus agalactiae (n= 324, 26.2%), Escherichia coli (n= 287, 23.2%), andStaphylococcus aureus (n= 131, 10.6%). The serotypes of Streptococcus agalactiae were serotype II (53.6%), Ia (44 %)and VII (1.2%), respectively. Streptococcus agalactiae were resistant to kanamycin (93.8%), gentamicin (49.4%), vancomycin (49.4%), tetracycline (35.8%), clindamycin (34.6%) and erythromycin (32.1%). The main resistance genes wereermA (53.1%) and ermB (85.2%). Resistance to erythromycin was attributed to the genes ermA (P < 0.05) and resistanceto tetracycline was attributed to the genes tetK, tetM, tetO (P < 0.01). The virulence genes scpB (81.4%), cyl (100%), glnA(76.6%), cfb (98.8%), hylB (98.8%), scaA (69.1%) were detected in almost all isolates.Discussion: In the present study, Streptococcus agalactiae, Escherichia coli and Staphylococcus aureus were the pathogens isolated most frequently from clinical mastitis. In the case of S. agalactiae, we performed capsular serotyping ofisolates...(AU)
Subject(s)
Animals , Cattle , Mastitis, Bovine/epidemiology , Drug Resistance, Bacterial , Streptococcus agalactiae/isolation & purification , Serogroup , Virulence/genetics , ChinaABSTRACT
Background: Bovine mastitis, a global disease that is responsible for large economic losses each year due to lower milkyield and reduced milk quality. In some countries, especially in China, Streptococcus agalactiae has become one of themost frequently detected pathogen. Antibiotic treatment and vaccine immunization are important strategies for the controlof infectious diseases. The main objective of the present study was to evaluate distribution of bovine mastitis pathogensand antimicrobial resistance of S. agalactiae, and contribute to the treatment of bovine mastitis.Materials, Methods & Results: Clinical mastitis samples (n= 1,122) were collected from 27 dairy farms located in 15different provinces of China during 2012-2018. The pathogens were identified by 16S rDNA method. Antimicrobial susceptibility was assessed by disc diffusion method. Molecular characteristics was distinguished based on PCR. The resultsshowed that the main pathogens were Streptococcus agalactiae (n= 324, 26.2%), Escherichia coli (n= 287, 23.2%), andStaphylococcus aureus (n= 131, 10.6%). The serotypes of Streptococcus agalactiae were serotype II (53.6%), Ia (44 %)and VII (1.2%), respectively. Streptococcus agalactiae were resistant to kanamycin (93.8%), gentamicin (49.4%), vancomycin (49.4%), tetracycline (35.8%), clindamycin (34.6%) and erythromycin (32.1%). The main resistance genes wereermA (53.1%) and ermB (85.2%). Resistance to erythromycin was attributed to the genes ermA (P < 0.05) and resistanceto tetracycline was attributed to the genes tetK, tetM, tetO (P < 0.01). The virulence genes scpB (81.4%), cyl (100%), glnA(76.6%), cfb (98.8%), hylB (98.8%), scaA (69.1%) were detected in almost all isolates.Discussion: In the present study, Streptococcus agalactiae, Escherichia coli and Staphylococcus aureus were the pathogens isolated most frequently from clinical mastitis. In the case of S. agalactiae, we performed capsular serotyping ofisolates...